LOCUS       BBF10K_003384           2413 bp    DNA     circular     29-Apr-2025
ACCESSION   BBF10K_003384
VERSION     BBF10K_003384.1  
FEATURES             Location/Qualifiers
     misc_feature    5..93
                     /label=BT1_BCD2
     CDS             complement(1236..2095)
                     /label=AmpR
     promoter        complement(2097..2200)
                     /label=AmpR promoter
     primer_bind     2219..2235
                     /label=M13 fwd
     primer_bind     complement(374..389)
                     /label=M13 rev
     rep_origin      complement(457..1044)
                     /label=ColE1
     primer_bind     291..309
                     /label=SLiCE-for
     primer_bind     complement(2263..2280)
                     /label=SLiCE-rev
     misc_feature    1048..1237
                     /label=spacer
     CDS             408..458
                     /label=spacer
     terminator      324..371
                     /label=T7 terminator
     terminator      2236..2267
                     /label=tonB terminator
     misc_feature    286..2286
                     /label=pOpen_v3 backbone
     misc_feature    2407..2412
                     /label=BsaI_site
     misc_feature    complement(99..104)
                     /label=BsaI_site
     misc_feature    1..4
                     /label=TACT
     misc_feature    94..97
                     /label=CCAT
ORIGIN
        1 TACTGGGCCC AAGTTCACTT AAAAAGGAGA TCAACAATGA AAGCAATTTT CGTACTGAAA
       61 CATCTTAATC ATGCTAAGGA GGTTTTCTAA TGTCCATCGA GACCACCGTG ATGGCTGTGC
      121 TTGGAGTCAA TCGCATGTAG GATGCTCTCC AGACACCGGG GCACCAGTTT TCACGCCCAA
      181 AGCATAAACG ACGAGCAGTC ATGAACAGTC CTTGCCAATC GAAGTATACG AAAATGGTAA
      241 TAGCACTAGG GATAACAGGG TAATCACTAT TACTAACAAC GTGTTCGCTG AGGTGTCAAT
      301 CGTCGGAGCC GCTGAGCAAT AACTAGCATA ACCCCTTGGG GCCTCTAAAC GGGTCTTGAG
      361 GGGTTTTTTG CATGGTCATA GCTGTTTCCT GAGAGCTTGG CAGGTGATGA CACACATTAA
      421 CAAATTTCGT GAGGAGTCTC CAGAAGAATG CCATTAATTT CCATAGGCTC CGCCCCCCTG
      481 ACGAGCATCA CAAAAATCGA CGCTCAAGTC AGAGGTGGCG AAACCCGACA GGACTATAAA
      541 GATACCAGGC GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC TCCTGTTCCG ACCCTGCCGC
      601 TTACCGGATA CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT GGCGCTTTCT CATAGCTCAC
      661 GCTGTAGGTA TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA GCTGGGCTGT GTGCACGAAC
      721 CCCCCGTTCA GCCCGACCGC TGCGCCTTAT CCGGTAACTA TCGTCTTGAG TCCAACCCGG
      781 TAAGACACGA CTTATCGCCA CTGGCAGCAG CCACTGGTAA CAGGATTAGC AGAGCGAGGT
      841 ATGTAGGCGG TGCTACAGAG TTCTTGAAGT GGTGGCCTAA CTACGGCTAC ACTAGAAGAA
      901 CAGTATTTGG TATCTGCGCT CTGCTGAAGC CAGTTACCTT CGGAAAAAGA GTTGGTAGCT
      961 CTTGATCCGG CAAACAAACC ACCGCTGGTA GCGGTGGTTT TTTTGTTTGC AAGCAGCAGA
     1021 TTACGCGCAG AAAAAAAGGA TCTCAAGAAG GCCTACTATT AGCAACAACG ATCCTTTGAT
     1081 CTTTTCTACG GGGTCTGACG CTCAGTGGAA CGAAAACTCA CGTTAAGGGA TTTTGGTCAT
     1141 GAGATTATCA AAAAGGATCT TCACCTAGAT CCTTTTAAAT TAAAAATGAA GTTTTAAATC
     1201 AATCTAAAGT ATATATGAGT AAACTTGGTC TGACAGTTAC CAATGCTTAA TCAGTGAGGC
     1261 ACCTATCTCA GCGATCTGTC TATTTCGTTC ATCCATAGTT GCCTGACTCC CCGTCGTGTA
     1321 GATAACTACG ATACGGGAGG GCTTACCATC TGGCCCCAGT GCTGCAATGA TACCGCGAGA
     1381 ACCACGCTCA CCGGCTCCAG ATTTATCAGC AATAAACCAG CCAGCCGGAA GGGCCGAGCG
     1441 CAGAAGTGGT CCTGCAACTT TATCCGCCTC CATCCAGTCT ATTAATTGTT GCCGGGAAGC
     1501 TAGAGTAAGT AGTTCGCCAG TTAATAGTTT GCGCAACGTT GTTGCCATTG CTACAGGCAT
     1561 CGTGGTGTCA CGCTCGTCGT TTGGTATGGC TTCATTCAGC TCCGGTTCCC AACGATCAAG
     1621 GCGAGTTACA TGATCCCCCA TGTTGTGCAA AAAAGCGGTT AGCTCCTTCG GTCCTCCGAT
     1681 CGTTGTCAGA AGTAAGTTGG CCGCAGTGTT ATCACTCATG GTTATGGCAG CACTGCATAA
     1741 TTCTCTTACT GTCATGCCAT CCGTAAGATG CTTTTCTGTG ACTGGTGAGT ACTCAACCAA
     1801 GTCATTCTGA GAATAGTGTA TGCGGCGACC GAGTTGCTCT TGCCCGGCGT CAATACGGGA
     1861 TAATACCGCG CCACATAGCA GAACTTTAAA AGTGCTCATC ATTGGAAAAC GTTCTTCGGG
     1921 GCGAAAACTC TCAAGGATCT TACCGCTGTT GAGATCCAGT TCGATGTAAC CCACTCGTGC
     1981 ACCCAACTGA TCTTCAGCAT CTTTTACTTT CACCAGCGTT TCTGGGTGAG CAAAAACAGG
     2041 AAGGCAAAAT GCCGCAAAAA AGGGAATAAG GGCGACACGG AAATGTTGAA TACTCATACT
     2101 CTTCCTTTTT CAATATTATT GAAGCATTTA TCAGGGTTAT TGTCTCATGA GCGGATACAT
     2161 ATTTGAATGT ATTTAGAAAA ATAAACAAAT AGGGGTTCCG CGCACCTGCA CCAGTCAGTA
     2221 AAACGACGGC CAGTAGTCAA AAGCCTCCGA CCGGAGGCTT TTGACTTGGT TCAGGTGGAG
     2281 TGGGAGAACG ATGATGCTCA CTCTCGTACA GCCAAACGAC CAAGTGCATT TCCACGGAGC
     2341 GCGATGGAGA TTCATTCACT CGGCAGCTCT GTAATAGGGA CTAAAAGAGT GATGATAATC
     2401 ATGAGTGGTC TCA
//