LOCUS       BBF10K_003385           2430 bp    DNA     circular     29-Apr-2025
ACCESSION   BBF10K_003385
VERSION     BBF10K_003385.1  
FEATURES             Location/Qualifiers
     misc_feature    5..118
                     /label=BT1_Riboj54_F1
     CDS             complement(1261..2120)
                     /label=AmpR
     promoter        complement(2122..2225)
                     /label=AmpR promoter
     primer_bind     2244..2260
                     /label=M13 fwd
     primer_bind     complement(399..414)
                     /label=M13 rev
     rep_origin      complement(482..1069)
                     /label=ColE1
     primer_bind     316..334
                     /label=SLiCE-for
     primer_bind     complement(2288..2305)
                     /label=SLiCE-rev
     misc_feature    1073..1262
                     /label=spacer
     CDS             433..483
                     /label=spacer
     terminator      349..396
                     /label=T7 terminator
     terminator      2261..2292
                     /label=tonB terminator
     misc_feature    311..2311
                     /label=pOpen_v3 backbone
     misc_feature    2424..2429
                     /label=BsaI_site
     misc_feature    complement(124..129)
                     /label=BsaI_site
     misc_feature    1..4
                     /label=TACT
     misc_feature    119..122
                     /label=CCAT
ORIGIN
        1 TACTAGGGGT CAGTTGATGT GCTTTCAACT CTGATGAGTC AGTGATGACG AAACCCCCTC
       61 TACAAATAAT TTTGTTTAAC TATGGACTAT GTTTTCACAT ACGAGGGGGA TTAGATGTCC
      121 ATCGAGACCC GATAGCCCAG TATGATATTC TAAGGCGTTA CGCTGATGAA TATTCTACGG
      181 AATTGCCATA GGCGTTGAAC GCTACACGGA CGATACGAAT TTATGTATAA CCCCGTGAAT
      241 GCTATGTTCT ACGATAGTAC GACTTGTACT CTAGGGATAA CAGGGTAATT CGTATCCGGG
      301 TTTCCCTCTA CGCTGAGGTG TCAATCGTCG GAGCCGCTGA GCAATAACTA GCATAACCCC
      361 TTGGGGCCTC TAAACGGGTC TTGAGGGGTT TTTTGCATGG TCATAGCTGT TTCCTGAGAG
      421 CTTGGCAGGT GATGACACAC ATTAACAAAT TTCGTGAGGA GTCTCCAGAA GAATGCCATT
      481 AATTTCCATA GGCTCCGCCC CCCTGACGAG CATCACAAAA ATCGACGCTC AAGTCAGAGG
      541 TGGCGAAACC CGACAGGACT ATAAAGATAC CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG
      601 CGCTCTCCTG TTCCGACCCT GCCGCTTACC GGATACCTGT CCGCCTTTCT CCCTTCGGGA
      661 AGCGTGGCGC TTTCTCATAG CTCACGCTGT AGGTATCTCA GTTCGGTGTA GGTCGTTCGC
      721 TCCAAGCTGG GCTGTGTGCA CGAACCCCCC GTTCAGCCCG ACCGCTGCGC CTTATCCGGT
      781 AACTATCGTC TTGAGTCCAA CCCGGTAAGA CACGACTTAT CGCCACTGGC AGCAGCCACT
      841 GGTAACAGGA TTAGCAGAGC GAGGTATGTA GGCGGTGCTA CAGAGTTCTT GAAGTGGTGG
      901 CCTAACTACG GCTACACTAG AAGAACAGTA TTTGGTATCT GCGCTCTGCT GAAGCCAGTT
      961 ACCTTCGGAA AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC AAACCACCGC TGGTAGCGGT
     1021 GGTTTTTTTG TTTGCAAGCA GCAGATTACG CGCAGAAAAA AAGGATCTCA AGAAGGCCTA
     1081 CTATTAGCAA CAACGATCCT TTGATCTTTT CTACGGGGTC TGACGCTCAG TGGAACGAAA
     1141 ACTCACGTTA AGGGATTTTG GTCATGAGAT TATCAAAAAG GATCTTCACC TAGATCCTTT
     1201 TAAATTAAAA ATGAAGTTTT AAATCAATCT AAAGTATATA TGAGTAAACT TGGTCTGACA
     1261 GTTACCAATG CTTAATCAGT GAGGCACCTA TCTCAGCGAT CTGTCTATTT CGTTCATCCA
     1321 TAGTTGCCTG ACTCCCCGTC GTGTAGATAA CTACGATACG GGAGGGCTTA CCATCTGGCC
     1381 CCAGTGCTGC AATGATACCG CGAGAACCAC GCTCACCGGC TCCAGATTTA TCAGCAATAA
     1441 ACCAGCCAGC CGGAAGGGCC GAGCGCAGAA GTGGTCCTGC AACTTTATCC GCCTCCATCC
     1501 AGTCTATTAA TTGTTGCCGG GAAGCTAGAG TAAGTAGTTC GCCAGTTAAT AGTTTGCGCA
     1561 ACGTTGTTGC CATTGCTACA GGCATCGTGG TGTCACGCTC GTCGTTTGGT ATGGCTTCAT
     1621 TCAGCTCCGG TTCCCAACGA TCAAGGCGAG TTACATGATC CCCCATGTTG TGCAAAAAAG
     1681 CGGTTAGCTC CTTCGGTCCT CCGATCGTTG TCAGAAGTAA GTTGGCCGCA GTGTTATCAC
     1741 TCATGGTTAT GGCAGCACTG CATAATTCTC TTACTGTCAT GCCATCCGTA AGATGCTTTT
     1801 CTGTGACTGG TGAGTACTCA ACCAAGTCAT TCTGAGAATA GTGTATGCGG CGACCGAGTT
     1861 GCTCTTGCCC GGCGTCAATA CGGGATAATA CCGCGCCACA TAGCAGAACT TTAAAAGTGC
     1921 TCATCATTGG AAAACGTTCT TCGGGGCGAA AACTCTCAAG GATCTTACCG CTGTTGAGAT
     1981 CCAGTTCGAT GTAACCCACT CGTGCACCCA ACTGATCTTC AGCATCTTTT ACTTTCACCA
     2041 GCGTTTCTGG GTGAGCAAAA ACAGGAAGGC AAAATGCCGC AAAAAAGGGA ATAAGGGCGA
     2101 CACGGAAATG TTGAATACTC ATACTCTTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG
     2161 GTTATTGTCT CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA CAAATAGGGG
     2221 TTCCGCGCAC CTGCACCAGT CAGTAAAACG ACGGCCAGTA GTCAAAAGCC TCCGACCGGA
     2281 GGCTTTTGAC TTGGTTCAGG TGGAGTGGGA GAACGATGAT GCTCACTCTC GGTTTTAAGA
     2341 CAGGAGTTCG CAAAATCAAG CGGGGTCATT ACAACGGTTA TTCCTGGTGG TTTAGGCGTA
     2401 CAATGTCCTG AAGAATATTT AAGGGTCTCA
//