LOCUS       BBF10K_003386           2427 bp    DNA     circular     17-Jun-2025
ACCESSION   BBF10K_003386
VERSION     BBF10K_003386.1  
FEATURES             Location/Qualifiers
     misc_feature    5..107
                     /label=BT1_Riboj_B34
     CDS             complement(1250..2109)
                     /label=AmpR
     promoter        complement(2111..2214)
                     /label=AmpR promoter
     primer_bind     2233..2249
                     /label=M13 fwd
     primer_bind     complement(388..403)
                     /label=M13 rev
     rep_origin      complement(471..1058)
                     /label=ColE1
     primer_bind     305..323
                     /label=SLiCE-for
     primer_bind     complement(2277..2294)
                     /label=SLiCE-rev
     misc_feature    1062..1251
                     /label=spacer
     CDS             422..472
                     /label=spacer
     terminator      338..385
                     /label=T7 terminator
     terminator      2250..2281
                     /label=tonB terminator
     misc_feature    300..2300
                     /label=pOpen_v3 backbone
     misc_feature    2421..2426
                     /label=BsaI_site
     misc_feature    complement(113..118)
                     /label=BsaI_site
     misc_feature    1..4
                     /label=TACT
     misc_feature    108..111
                     /label=CCAT
ORIGIN
        1 TACTAGCTGT CACCGGATGT GCTTTCCGGT CTGATGAGTC CGTGAGGACG AAACAGCCTC
       61 TACAAATAAT TTTGTTTAAA CTAGAAAAGA GGAGAAATAC TAGATGTCCA TCGAGACCTT
      121 CGCACTGTCA GGGTCACTTG GGTGTTTTGC ACTACCGTCA GGTACGCTAG TATGCGTTCT
      181 TCCTTCCAGA GGTATGTGGC TGCGTGGTCA AAAGTGCGAC CAGCCTCAGA TTGTTGTCTA
      241 CAGTCAGACA GTTGGGTTCT TAGGGATAAC AGGGTAATGA TCACTTTATT CGCACAGGCC
      301 GCTGAGGTGT CAATCGTCGG AGCCGCTGAG CAATAACTAG CATAACCCCT TGGGGCCTCT
      361 AAACGGGTCT TGAGGGGTTT TTTGCATGGT CATAGCTGTT TCCTGAGAGC TTGGCAGGTG
      421 ATGACACACA TTAACAAATT TCGTGAGGAG TCTCCAGAAG AATGCCATTA ATTTCCATAG
      481 GCTCCGCCCC CCTGACGAGC ATCACAAAAA TCGACGCTCA AGTCAGAGGT GGCGAAACCC
      541 GACAGGACTA TAAAGATACC AGGCGTTTCC CCCTGGAAGC TCCCTCGTGC GCTCTCCTGT
      601 TCCGACCCTG CCGCTTACCG GATACCTGTC CGCCTTTCTC CCTTCGGGAA GCGTGGCGCT
      661 TTCTCATAGC TCACGCTGTA GGTATCTCAG TTCGGTGTAG GTCGTTCGCT CCAAGCTGGG
      721 CTGTGTGCAC GAACCCCCCG TTCAGCCCGA CCGCTGCGCC TTATCCGGTA ACTATCGTCT
      781 TGAGTCCAAC CCGGTAAGAC ACGACTTATC GCCACTGGCA GCAGCCACTG GTAACAGGAT
      841 TAGCAGAGCG AGGTATGTAG GCGGTGCTAC AGAGTTCTTG AAGTGGTGGC CTAACTACGG
      901 CTACACTAGA AGAACAGTAT TTGGTATCTG CGCTCTGCTG AAGCCAGTTA CCTTCGGAAA
      961 AAGAGTTGGT AGCTCTTGAT CCGGCAAACA AACCACCGCT GGTAGCGGTG GTTTTTTTGT
     1021 TTGCAAGCAG CAGATTACGC GCAGAAAAAA AGGATCTCAA GAAGGCCTAC TATTAGCAAC
     1081 AACGATCCTT TGATCTTTTC TACGGGGTCT GACGCTCAGT GGAACGAAAA CTCACGTTAA
     1141 GGGATTTTGG TCATGAGATT ATCAAAAAGG ATCTTCACCT AGATCCTTTT AAATTAAAAA
     1201 TGAAGTTTTA AATCAATCTA AAGTATATAT GAGTAAACTT GGTCTGACAG TTACCAATGC
     1261 TTAATCAGTG AGGCACCTAT CTCAGCGATC TGTCTATTTC GTTCATCCAT AGTTGCCTGA
     1321 CTCCCCGTCG TGTAGATAAC TACGATACGG GAGGGCTTAC CATCTGGCCC CAGTGCTGCA
     1381 ATGATACCGC GAGAACCACG CTCACCGGCT CCAGATTTAT CAGCAATAAA CCAGCCAGCC
     1441 GGAAGGGCCG AGCGCAGAAG TGGTCCTGCA ACTTTATCCG CCTCCATCCA GTCTATTAAT
     1501 TGTTGCCGGG AAGCTAGAGT AAGTAGTTCG CCAGTTAATA GTTTGCGCAA CGTTGTTGCC
     1561 ATTGCTACAG GCATCGTGGT GTCACGCTCG TCGTTTGGTA TGGCTTCATT CAGCTCCGGT
     1621 TCCCAACGAT CAAGGCGAGT TACATGATCC CCCATGTTGT GCAAAAAAGC GGTTAGCTCC
     1681 TTCGGTCCTC CGATCGTTGT CAGAAGTAAG TTGGCCGCAG TGTTATCACT CATGGTTATG
     1741 GCAGCACTGC ATAATTCTCT TACTGTCATG CCATCCGTAA GATGCTTTTC TGTGACTGGT
     1801 GAGTACTCAA CCAAGTCATT CTGAGAATAG TGTATGCGGC GACCGAGTTG CTCTTGCCCG
     1861 GCGTCAATAC GGGATAATAC CGCGCCACAT AGCAGAACTT TAAAAGTGCT CATCATTGGA
     1921 AAACGTTCTT CGGGGCGAAA ACTCTCAAGG ATCTTACCGC TGTTGAGATC CAGTTCGATG
     1981 TAACCCACTC GTGCACCCAA CTGATCTTCA GCATCTTTTA CTTTCACCAG CGTTTCTGGG
     2041 TGAGCAAAAA CAGGAAGGCA AAATGCCGCA AAAAAGGGAA TAAGGGCGAC ACGGAAATGT
     2101 TGAATACTCA TACTCTTCCT TTTTCAATAT TATTGAAGCA TTTATCAGGG TTATTGTCTC
     2161 ATGAGCGGAT ACATATTTGA ATGTATTTAG AAAAATAAAC AAATAGGGGT TCCGCGCACC
     2221 TGCACCAGTC AGTAAAACGA CGGCCAGTAG TCAAAAGCCT CCGACCGGAG GCTTTTGACT
     2281 TGGTTCAGGT GGAGTGGGAG AACGATGATG CTCACTCTCG GCATTCGTAT TTGCTCCTCG
     2341 TGTTTACTCT CACAAACTTG ACCTGGAGAT CAAGGAGATG CTTCTTGTGG AACTGGACAA
     2401 CGCATCAACG CAACGGATCT GGTCTCA
//