LOCUS       BBF10K_003389           2443 bp    DNA     circular     29-Apr-2025
ACCESSION   BBF10K_003389
VERSION     BBF10K_003389.1  
FEATURES             Location/Qualifiers
     misc_feature    5..123
                     /label=BT1_BCD2_His
     CDS             complement(1266..2125)
                     /label=AmpR
     promoter        complement(2127..2230)
                     /label=AmpR promoter
     primer_bind     2249..2265
                     /label=M13 fwd
     primer_bind     complement(404..419)
                     /label=M13 rev
     rep_origin      complement(487..1074)
                     /label=ColE1
     primer_bind     321..339
                     /label=SLiCE-for
     primer_bind     complement(2293..2310)
                     /label=SLiCE-rev
     misc_feature    1078..1267
                     /label=spacer
     CDS             438..488
                     /label=spacer
     terminator      354..401
                     /label=T7 terminator
     terminator      2266..2297
                     /label=tonB terminator
     misc_feature    316..2316
                     /label=pOpen_v3 backbone
     misc_feature    2437..2442
                     /label=BsaI_site
     misc_feature    complement(129..134)
                     /label=BsaI_site
     misc_feature    1..4
                     /label=TACT
     misc_feature    124..127
                     /label=CCAT
ORIGIN
        1 TACTGGGCCC AAGTTCACTT AAAAAGGAGA TCAACAATGA AAGCAATTTT CGTACTGAAA
       61 CATCTTAATC ATGCTAAGGA GGTTTTCTAA TGGGTAGCAG CCATCATCAT CATCATCACG
      121 GCTCCATCGA GACCACCGTG ATGGCTGTGC TTGGAGTCAA TCGCATGTAG GATGCTCTCC
      181 AGACACCGGG GCACCAGTTT TCACGCCCAA AGCATAAACG ACGAGCAGTC ATGAAGTGTG
      241 GTCAATCCCA TGTCTAGTCA GCGTGGCGGG CTCAAGTAGG GATAACAGGG TAATCTGCGT
      301 ATGCTAAATG CTGTTCGCTG AGGTGTCAAT CGTCGGAGCC GCTGAGCAAT AACTAGCATA
      361 ACCCCTTGGG GCCTCTAAAC GGGTCTTGAG GGGTTTTTTG CATGGTCATA GCTGTTTCCT
      421 GAGAGCTTGG CAGGTGATGA CACACATTAA CAAATTTCGT GAGGAGTCTC CAGAAGAATG
      481 CCATTAATTT CCATAGGCTC CGCCCCCCTG ACGAGCATCA CAAAAATCGA CGCTCAAGTC
      541 AGAGGTGGCG AAACCCGACA GGACTATAAA GATACCAGGC GTTTCCCCCT GGAAGCTCCC
      601 TCGTGCGCTC TCCTGTTCCG ACCCTGCCGC TTACCGGATA CCTGTCCGCC TTTCTCCCTT
      661 CGGGAAGCGT GGCGCTTTCT CATAGCTCAC GCTGTAGGTA TCTCAGTTCG GTGTAGGTCG
      721 TTCGCTCCAA GCTGGGCTGT GTGCACGAAC CCCCCGTTCA GCCCGACCGC TGCGCCTTAT
      781 CCGGTAACTA TCGTCTTGAG TCCAACCCGG TAAGACACGA CTTATCGCCA CTGGCAGCAG
      841 CCACTGGTAA CAGGATTAGC AGAGCGAGGT ATGTAGGCGG TGCTACAGAG TTCTTGAAGT
      901 GGTGGCCTAA CTACGGCTAC ACTAGAAGAA CAGTATTTGG TATCTGCGCT CTGCTGAAGC
      961 CAGTTACCTT CGGAAAAAGA GTTGGTAGCT CTTGATCCGG CAAACAAACC ACCGCTGGTA
     1021 GCGGTGGTTT TTTTGTTTGC AAGCAGCAGA TTACGCGCAG AAAAAAAGGA TCTCAAGAAG
     1081 GCCTACTATT AGCAACAACG ATCCTTTGAT CTTTTCTACG GGGTCTGACG CTCAGTGGAA
     1141 CGAAAACTCA CGTTAAGGGA TTTTGGTCAT GAGATTATCA AAAAGGATCT TCACCTAGAT
     1201 CCTTTTAAAT TAAAAATGAA GTTTTAAATC AATCTAAAGT ATATATGAGT AAACTTGGTC
     1261 TGACAGTTAC CAATGCTTAA TCAGTGAGGC ACCTATCTCA GCGATCTGTC TATTTCGTTC
     1321 ATCCATAGTT GCCTGACTCC CCGTCGTGTA GATAACTACG ATACGGGAGG GCTTACCATC
     1381 TGGCCCCAGT GCTGCAATGA TACCGCGAGA ACCACGCTCA CCGGCTCCAG ATTTATCAGC
     1441 AATAAACCAG CCAGCCGGAA GGGCCGAGCG CAGAAGTGGT CCTGCAACTT TATCCGCCTC
     1501 CATCCAGTCT ATTAATTGTT GCCGGGAAGC TAGAGTAAGT AGTTCGCCAG TTAATAGTTT
     1561 GCGCAACGTT GTTGCCATTG CTACAGGCAT CGTGGTGTCA CGCTCGTCGT TTGGTATGGC
     1621 TTCATTCAGC TCCGGTTCCC AACGATCAAG GCGAGTTACA TGATCCCCCA TGTTGTGCAA
     1681 AAAAGCGGTT AGCTCCTTCG GTCCTCCGAT CGTTGTCAGA AGTAAGTTGG CCGCAGTGTT
     1741 ATCACTCATG GTTATGGCAG CACTGCATAA TTCTCTTACT GTCATGCCAT CCGTAAGATG
     1801 CTTTTCTGTG ACTGGTGAGT ACTCAACCAA GTCATTCTGA GAATAGTGTA TGCGGCGACC
     1861 GAGTTGCTCT TGCCCGGCGT CAATACGGGA TAATACCGCG CCACATAGCA GAACTTTAAA
     1921 AGTGCTCATC ATTGGAAAAC GTTCTTCGGG GCGAAAACTC TCAAGGATCT TACCGCTGTT
     1981 GAGATCCAGT TCGATGTAAC CCACTCGTGC ACCCAACTGA TCTTCAGCAT CTTTTACTTT
     2041 CACCAGCGTT TCTGGGTGAG CAAAAACAGG AAGGCAAAAT GCCGCAAAAA AGGGAATAAG
     2101 GGCGACACGG AAATGTTGAA TACTCATACT CTTCCTTTTT CAATATTATT GAAGCATTTA
     2161 TCAGGGTTAT TGTCTCATGA GCGGATACAT ATTTGAATGT ATTTAGAAAA ATAAACAAAT
     2221 AGGGGTTCCG CGCACCTGCA CCAGTCAGTA AAACGACGGC CAGTAGTCAA AAGCCTCCGA
     2281 CCGGAGGCTT TTGACTTGGT TCAGGTGGAG TGGGAGAACG ATGATGCTCA CTCTCGTACA
     2341 GCCAAACGAC CAAGTGCATT TCCACGGAGC GCGATGGAGA TTCATTCACT CGGCAGCTCT
     2401 GTAATAGGGA CTAAAAGAGT GATGATAATC ATGAGTGGTC TCA
//