LOCUS       BBF10K_003390           2457 bp    DNA     circular     29-Apr-2025
ACCESSION   BBF10K_003390
VERSION     BBF10K_003390.1  
FEATURES             Location/Qualifiers
     misc_feature    5..137
                     /label=BT1_RiboJ_B34_His
     CDS             complement(1280..2139)
                     /label=AmpR
     promoter        complement(2141..2244)
                     /label=AmpR promoter
     primer_bind     2263..2279
                     /label=M13 fwd
     primer_bind     complement(418..433)
                     /label=M13 rev
     rep_origin      complement(501..1088)
                     /label=ColE1
     primer_bind     335..353
                     /label=SLiCE-for
     primer_bind     complement(2307..2324)
                     /label=SLiCE-rev
     misc_feature    1092..1281
                     /label=spacer
     CDS             452..502
                     /label=spacer
     terminator      368..415
                     /label=T7 terminator
     terminator      2280..2311
                     /label=tonB terminator
     misc_feature    330..2330
                     /label=pOpen_v3 backbone
     misc_feature    2451..2456
                     /label=BsaI_site
     misc_feature    complement(143..148)
                     /label=BsaI_site
     misc_feature    1..4
                     /label=TACT
     misc_feature    138..141
                     /label=CCAT
ORIGIN
        1 TACTAGCTGT CACCGGATGT GCTTTCCGGT CTGATGAGTC CGTGAGGACG AAACAGCCTC
       61 TACAAATAAT TTTGTTTAAA CTAGAAAAGA GGAGAAATAC TAGATGGGCA GCAGCCATCA
      121 TCATCATCAT CACGGCTCCA TCGAGACCTT CGCACTGTCA GGGTCACTTG GGTGTTTTGC
      181 ACTACCGTCA GGTACGCTAG TATGCGTTCT TCCTTCCAGA GGTATGTGGC TGCGTGGTCA
      241 AAAGTGCGAG AGGTCAATCA TGCCTCTCTT ACCGACCAGG GGCAACGTAT TAGGGATAAC
      301 AGGGTAATAC CCATCGATCC CGCAGTAATC GCTGAGGTGT CAATCGTCGG AGCCGCTGAG
      361 CAATAACTAG CATAACCCCT TGGGGCCTCT AAACGGGTCT TGAGGGGTTT TTTGCATGGT
      421 CATAGCTGTT TCCTGAGAGC TTGGCAGGTG ATGACACACA TTAACAAATT TCGTGAGGAG
      481 TCTCCAGAAG AATGCCATTA ATTTCCATAG GCTCCGCCCC CCTGACGAGC ATCACAAAAA
      541 TCGACGCTCA AGTCAGAGGT GGCGAAACCC GACAGGACTA TAAAGATACC AGGCGTTTCC
      601 CCCTGGAAGC TCCCTCGTGC GCTCTCCTGT TCCGACCCTG CCGCTTACCG GATACCTGTC
      661 CGCCTTTCTC CCTTCGGGAA GCGTGGCGCT TTCTCATAGC TCACGCTGTA GGTATCTCAG
      721 TTCGGTGTAG GTCGTTCGCT CCAAGCTGGG CTGTGTGCAC GAACCCCCCG TTCAGCCCGA
      781 CCGCTGCGCC TTATCCGGTA ACTATCGTCT TGAGTCCAAC CCGGTAAGAC ACGACTTATC
      841 GCCACTGGCA GCAGCCACTG GTAACAGGAT TAGCAGAGCG AGGTATGTAG GCGGTGCTAC
      901 AGAGTTCTTG AAGTGGTGGC CTAACTACGG CTACACTAGA AGAACAGTAT TTGGTATCTG
      961 CGCTCTGCTG AAGCCAGTTA CCTTCGGAAA AAGAGTTGGT AGCTCTTGAT CCGGCAAACA
     1021 AACCACCGCT GGTAGCGGTG GTTTTTTTGT TTGCAAGCAG CAGATTACGC GCAGAAAAAA
     1081 AGGATCTCAA GAAGGCCTAC TATTAGCAAC AACGATCCTT TGATCTTTTC TACGGGGTCT
     1141 GACGCTCAGT GGAACGAAAA CTCACGTTAA GGGATTTTGG TCATGAGATT ATCAAAAAGG
     1201 ATCTTCACCT AGATCCTTTT AAATTAAAAA TGAAGTTTTA AATCAATCTA AAGTATATAT
     1261 GAGTAAACTT GGTCTGACAG TTACCAATGC TTAATCAGTG AGGCACCTAT CTCAGCGATC
     1321 TGTCTATTTC GTTCATCCAT AGTTGCCTGA CTCCCCGTCG TGTAGATAAC TACGATACGG
     1381 GAGGGCTTAC CATCTGGCCC CAGTGCTGCA ATGATACCGC GAGAACCACG CTCACCGGCT
     1441 CCAGATTTAT CAGCAATAAA CCAGCCAGCC GGAAGGGCCG AGCGCAGAAG TGGTCCTGCA
     1501 ACTTTATCCG CCTCCATCCA GTCTATTAAT TGTTGCCGGG AAGCTAGAGT AAGTAGTTCG
     1561 CCAGTTAATA GTTTGCGCAA CGTTGTTGCC ATTGCTACAG GCATCGTGGT GTCACGCTCG
     1621 TCGTTTGGTA TGGCTTCATT CAGCTCCGGT TCCCAACGAT CAAGGCGAGT TACATGATCC
     1681 CCCATGTTGT GCAAAAAAGC GGTTAGCTCC TTCGGTCCTC CGATCGTTGT CAGAAGTAAG
     1741 TTGGCCGCAG TGTTATCACT CATGGTTATG GCAGCACTGC ATAATTCTCT TACTGTCATG
     1801 CCATCCGTAA GATGCTTTTC TGTGACTGGT GAGTACTCAA CCAAGTCATT CTGAGAATAG
     1861 TGTATGCGGC GACCGAGTTG CTCTTGCCCG GCGTCAATAC GGGATAATAC CGCGCCACAT
     1921 AGCAGAACTT TAAAAGTGCT CATCATTGGA AAACGTTCTT CGGGGCGAAA ACTCTCAAGG
     1981 ATCTTACCGC TGTTGAGATC CAGTTCGATG TAACCCACTC GTGCACCCAA CTGATCTTCA
     2041 GCATCTTTTA CTTTCACCAG CGTTTCTGGG TGAGCAAAAA CAGGAAGGCA AAATGCCGCA
     2101 AAAAAGGGAA TAAGGGCGAC ACGGAAATGT TGAATACTCA TACTCTTCCT TTTTCAATAT
     2161 TATTGAAGCA TTTATCAGGG TTATTGTCTC ATGAGCGGAT ACATATTTGA ATGTATTTAG
     2221 AAAAATAAAC AAATAGGGGT TCCGCGCACC TGCACCAGTC AGTAAAACGA CGGCCAGTAG
     2281 TCAAAAGCCT CCGACCGGAG GCTTTTGACT TGGTTCAGGT GGAGTGGGAG AACGATGATG
     2341 CTCACTCTCG GCATTCGTAT TTGCTCCTCG TGTTTACTCT CACAAACTTG ACCTGGAGAT
     2401 CAAGGAGATG CTTCTTGTGG AACTGGACAA CGCATCAACG CAACGGATCT GGTCTCA
//