LOCUS BBF10K_003391 2849 bp DNA circular 29-Apr-2025 ACCESSION BBF10K_003391 VERSION BBF10K_003391.1 FEATURES Location/Qualifiers misc_feature 5..729 /label=BT1_BCD2_DsbA CDS complement(1772..2631) /label=AmpR promoter complement(2633..2736) /label=AmpR promoter primer_bind 2755..2771 /label=M13 fwd primer_bind complement(910..925) /label=M13 rev rep_origin complement(993..1580) /label=ColE1 primer_bind 827..845 /label=SLiCE-for primer_bind complement(2799..2816) /label=SLiCE-rev misc_feature 1584..1773 /label=spacer CDS 944..994 /label=spacer terminator 860..907 /label=T7 terminator terminator 2772..2803 /label=tonB terminator misc_feature 822..2822 /label=pOpen_v3 backbone misc_feature 2843..2848 /label=BsaI_site misc_feature complement(735..740) /label=BsaI_site misc_feature 1..4 /label=TACT misc_feature 730..733 /label=CCAT ORIGIN 1 TACTGGGCCC AAGTTCACTT AAAAAGGAGA TCAACAATGA AAGCAATTTT CGTACTGAAA 61 CATCTTAATC ATGCTAAGGA GGTTTTCTAA TGAAAAAGAT TTGGCTGGCG CTGGCTGGTT 121 TAGTTTTAGC GTTTAGCGCA TCGGCGGCGC AGTATGAAGA TGGTAAACAG TACACTACCC 181 TGGAAAAACC GGTAGCTGGC GCGCCGCAAG TGCTGGAGTT TTTCTCTTTC TTCTGCCCGC 241 ACTGCTATCA GTTTGAAGAA GTTCTGCATA TTTCTGATAA TGTGAAGAAA AAACTGCCGG 301 AAGGCGTGAA GATGACTAAA TACCACGTCA ACTTCATGGG TGGTGACCTG GGCAAAGATC 361 TGACTCAGGC ATGGGCTGTG GCGATGGCGC TGGGCGTGGA GGACAAAGTG ACTGTTCCGC 421 TGTTTGAAGG CGTACAGAAA ACCCAGACCA TTCGTTCTGC TTCTGATATC CGCGATGTAT 481 TTATCAACGC AGGTATTAAA GGTGAAGAGT ACGACGCGGC GTGGAACAGC TTCGTGGTGA 541 AATCTCTGGT CGCTCAGCAG GAAAAAGCTG CAGCTGACGT GCAATTGCGT GGCGTTCCGG 601 CGATGTTTGT TAACGGTAAA TATCAGCTGA ATCCGCAGGG TATGGATACC AGCAATATGG 661 ATGTTTTTGT TCAGCAGTAT GCTGATACAG TGAAATACTT AAGCGAGAAA AAAGGATCAA 721 CGAGTGGTTC CATCGAGACC ACGGATTCTA GGTGCAACTC TTCTCATTCA GCGTCACACC 781 CGTAGGGATA ACAGGGTAAT GTCTTTCGAG ACTGTAATTG GCGCTGAGGT GTCAATCGTC 841 GGAGCCGCTG AGCAATAACT AGCATAACCC CTTGGGGCCT CTAAACGGGT CTTGAGGGGT 901 TTTTTGCATG GTCATAGCTG TTTCCTGAGA GCTTGGCAGG TGATGACACA CATTAACAAA 961 TTTCGTGAGG AGTCTCCAGA AGAATGCCAT TAATTTCCAT AGGCTCCGCC CCCCTGACGA 1021 GCATCACAAA AATCGACGCT CAAGTCAGAG GTGGCGAAAC CCGACAGGAC TATAAAGATA 1081 CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT GCGCTCTCCT GTTCCGACCC TGCCGCTTAC 1141 CGGATACCTG TCCGCCTTTC TCCCTTCGGG AAGCGTGGCG CTTTCTCATA GCTCACGCTG 1201 TAGGTATCTC AGTTCGGTGT AGGTCGTTCG CTCCAAGCTG GGCTGTGTGC ACGAACCCCC 1261 CGTTCAGCCC GACCGCTGCG CCTTATCCGG TAACTATCGT CTTGAGTCCA ACCCGGTAAG 1321 ACACGACTTA TCGCCACTGG CAGCAGCCAC TGGTAACAGG ATTAGCAGAG CGAGGTATGT 1381 AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG GCCTAACTAC GGCTACACTA GAAGAACAGT 1441 ATTTGGTATC TGCGCTCTGC TGAAGCCAGT TACCTTCGGA AAAAGAGTTG GTAGCTCTTG 1501 ATCCGGCAAA CAAACCACCG CTGGTAGCGG TGGTTTTTTT GTTTGCAAGC AGCAGATTAC 1561 GCGCAGAAAA AAAGGATCTC AAGAAGGCCT ACTATTAGCA ACAACGATCC TTTGATCTTT 1621 TCTACGGGGT CTGACGCTCA GTGGAACGAA AACTCACGTT AAGGGATTTT GGTCATGAGA 1681 TTATCAAAAA GGATCTTCAC CTAGATCCTT TTAAATTAAA AATGAAGTTT TAAATCAATC 1741 TAAAGTATAT ATGAGTAAAC TTGGTCTGAC AGTTACCAAT GCTTAATCAG TGAGGCACCT 1801 ATCTCAGCGA TCTGTCTATT TCGTTCATCC ATAGTTGCCT GACTCCCCGT CGTGTAGATA 1861 ACTACGATAC GGGAGGGCTT ACCATCTGGC CCCAGTGCTG CAATGATACC GCGAGAACCA 1921 CGCTCACCGG CTCCAGATTT ATCAGCAATA AACCAGCCAG CCGGAAGGGC CGAGCGCAGA 1981 AGTGGTCCTG CAACTTTATC CGCCTCCATC CAGTCTATTA ATTGTTGCCG GGAAGCTAGA 2041 GTAAGTAGTT CGCCAGTTAA TAGTTTGCGC AACGTTGTTG CCATTGCTAC AGGCATCGTG 2101 GTGTCACGCT CGTCGTTTGG TATGGCTTCA TTCAGCTCCG GTTCCCAACG ATCAAGGCGA 2161 GTTACATGAT CCCCCATGTT GTGCAAAAAA GCGGTTAGCT CCTTCGGTCC TCCGATCGTT 2221 GTCAGAAGTA AGTTGGCCGC AGTGTTATCA CTCATGGTTA TGGCAGCACT GCATAATTCT 2281 CTTACTGTCA TGCCATCCGT AAGATGCTTT TCTGTGACTG GTGAGTACTC AACCAAGTCA 2341 TTCTGAGAAT AGTGTATGCG GCGACCGAGT TGCTCTTGCC CGGCGTCAAT ACGGGATAAT 2401 ACCGCGCCAC ATAGCAGAAC TTTAAAAGTG CTCATCATTG GAAAACGTTC TTCGGGGCGA 2461 AAACTCTCAA GGATCTTACC GCTGTTGAGA TCCAGTTCGA TGTAACCCAC TCGTGCACCC 2521 AACTGATCTT CAGCATCTTT TACTTTCACC AGCGTTTCTG GGTGAGCAAA AACAGGAAGG 2581 CAAAATGCCG CAAAAAAGGG AATAAGGGCG ACACGGAAAT GTTGAATACT CATACTCTTC 2641 CTTTTTCAAT ATTATTGAAG CATTTATCAG GGTTATTGTC TCATGAGCGG ATACATATTT 2701 GAATGTATTT AGAAAAATAA ACAAATAGGG GTTCCGCGCA CCTGCACCAG TCAGTAAAAC 2761 GACGGCCAGT AGTCAAAAGC CTCCGACCGG AGGCTTTTGA CTTGGTTCAG GTGGAGTGGG 2821 AGAACGATGA TGCTCACTCT CGGGTCTCA //