LOCUS       BBF10K_003392           2441 bp    DNA     circular     29-Apr-2025
ACCESSION   BBF10K_003392
VERSION     BBF10K_003392.1  
FEATURES             Location/Qualifiers
     misc_feature    5..171
                     /label=BT1_BCD2_OmpT
     CDS             complement(1289..2148)
                     /label=AmpR
     promoter        complement(2150..2253)
                     /label=AmpR promoter
     primer_bind     2272..2288
                     /label=M13 fwd
     primer_bind     complement(427..442)
                     /label=M13 rev
     rep_origin      complement(510..1097)
                     /label=ColE1
     primer_bind     344..362
                     /label=SLiCE-for
     primer_bind     complement(2316..2333)
                     /label=SLiCE-rev
     misc_feature    1101..1290
                     /label=spacer
     CDS             461..511
                     /label=spacer
     terminator      377..424
                     /label=T7 terminator
     terminator      2289..2320
                     /label=tonB terminator
     misc_feature    339..2339
                     /label=pOpen_v3 backbone
     misc_feature    2435..2440
                     /label=BsaI_site
     misc_feature    complement(177..182)
                     /label=BsaI_site
     misc_feature    1..4
                     /label=TACT
     misc_feature    172..175
                     /label=CCAT
ORIGIN
        1 TACTGGGCCC AAGTTCACTT AAAAAGGAGA TCAACAATGA AAGCAATTTT CGTACTGAAA
       61 CATCTTAATC ATGCTAAGGA GGTTTTCTAA TGCGGGCGAA ACTCCTAGGA ATAGTCCTGA
      121 CAACCCCTAT CGCGATCAGC TCTTTTGCGT CGACGGGATC AACGAGTGGT TCCATCGAGA
      181 CCTGTATGCA CAAGGCCGAC AATAGGACGT AGCCTTCGAG TTAGTACGTA GCGTGGTCGC
      241 ATAAGCACAG TAGATCCAGC TTGACGGAGC ATCTATCTAG GTCATTAATA GTTTATGACT
      301 AGGGATAACA GGGTAATGAA AAACCTACTT TGAAAACTCG CTGAGGTGTC AATCGTCGGA
      361 GCCGCTGAGC AATAACTAGC ATAACCCCTT GGGGCCTCTA AACGGGTCTT GAGGGGTTTT
      421 TTGCATGGTC ATAGCTGTTT CCTGAGAGCT TGGCAGGTGA TGACACACAT TAACAAATTT
      481 CGTGAGGAGT CTCCAGAAGA ATGCCATTAA TTTCCATAGG CTCCGCCCCC CTGACGAGCA
      541 TCACAAAAAT CGACGCTCAA GTCAGAGGTG GCGAAACCCG ACAGGACTAT AAAGATACCA
      601 GGCGTTTCCC CCTGGAAGCT CCCTCGTGCG CTCTCCTGTT CCGACCCTGC CGCTTACCGG
      661 ATACCTGTCC GCCTTTCTCC CTTCGGGAAG CGTGGCGCTT TCTCATAGCT CACGCTGTAG
      721 GTATCTCAGT TCGGTGTAGG TCGTTCGCTC CAAGCTGGGC TGTGTGCACG AACCCCCCGT
      781 TCAGCCCGAC CGCTGCGCCT TATCCGGTAA CTATCGTCTT GAGTCCAACC CGGTAAGACA
      841 CGACTTATCG CCACTGGCAG CAGCCACTGG TAACAGGATT AGCAGAGCGA GGTATGTAGG
      901 CGGTGCTACA GAGTTCTTGA AGTGGTGGCC TAACTACGGC TACACTAGAA GAACAGTATT
      961 TGGTATCTGC GCTCTGCTGA AGCCAGTTAC CTTCGGAAAA AGAGTTGGTA GCTCTTGATC
     1021 CGGCAAACAA ACCACCGCTG GTAGCGGTGG TTTTTTTGTT TGCAAGCAGC AGATTACGCG
     1081 CAGAAAAAAA GGATCTCAAG AAGGCCTACT ATTAGCAACA ACGATCCTTT GATCTTTTCT
     1141 ACGGGGTCTG ACGCTCAGTG GAACGAAAAC TCACGTTAAG GGATTTTGGT CATGAGATTA
     1201 TCAAAAAGGA TCTTCACCTA GATCCTTTTA AATTAAAAAT GAAGTTTTAA ATCAATCTAA
     1261 AGTATATATG AGTAAACTTG GTCTGACAGT TACCAATGCT TAATCAGTGA GGCACCTATC
     1321 TCAGCGATCT GTCTATTTCG TTCATCCATA GTTGCCTGAC TCCCCGTCGT GTAGATAACT
     1381 ACGATACGGG AGGGCTTACC ATCTGGCCCC AGTGCTGCAA TGATACCGCG AGAACCACGC
     1441 TCACCGGCTC CAGATTTATC AGCAATAAAC CAGCCAGCCG GAAGGGCCGA GCGCAGAAGT
     1501 GGTCCTGCAA CTTTATCCGC CTCCATCCAG TCTATTAATT GTTGCCGGGA AGCTAGAGTA
     1561 AGTAGTTCGC CAGTTAATAG TTTGCGCAAC GTTGTTGCCA TTGCTACAGG CATCGTGGTG
     1621 TCACGCTCGT CGTTTGGTAT GGCTTCATTC AGCTCCGGTT CCCAACGATC AAGGCGAGTT
     1681 ACATGATCCC CCATGTTGTG CAAAAAAGCG GTTAGCTCCT TCGGTCCTCC GATCGTTGTC
     1741 AGAAGTAAGT TGGCCGCAGT GTTATCACTC ATGGTTATGG CAGCACTGCA TAATTCTCTT
     1801 ACTGTCATGC CATCCGTAAG ATGCTTTTCT GTGACTGGTG AGTACTCAAC CAAGTCATTC
     1861 TGAGAATAGT GTATGCGGCG ACCGAGTTGC TCTTGCCCGG CGTCAATACG GGATAATACC
     1921 GCGCCACATA GCAGAACTTT AAAAGTGCTC ATCATTGGAA AACGTTCTTC GGGGCGAAAA
     1981 CTCTCAAGGA TCTTACCGCT GTTGAGATCC AGTTCGATGT AACCCACTCG TGCACCCAAC
     2041 TGATCTTCAG CATCTTTTAC TTTCACCAGC GTTTCTGGGT GAGCAAAAAC AGGAAGGCAA
     2101 AATGCCGCAA AAAAGGGAAT AAGGGCGACA CGGAAATGTT GAATACTCAT ACTCTTCCTT
     2161 TTTCAATATT ATTGAAGCAT TTATCAGGGT TATTGTCTCA TGAGCGGATA CATATTTGAA
     2221 TGTATTTAGA AAAATAAACA AATAGGGGTT CCGCGCACCT GCACCAGTCA GTAAAACGAC
     2281 GGCCAGTAGT CAAAAGCCTC CGACCGGAGG CTTTTGACTT GGTTCAGGTG GAGTGGGAGA
     2341 ACGATGATGC TCACTCTCGT CCCCGCGCAT CCTATTTATT AAGTTAATTC TATAGCAATA
     2401 CGATCATATG CGGATGGGCA GTGGCCGGTA GTCAGGTCTC A
//