LOCUS       BBF10K_003393           2426 bp    DNA     circular     29-Apr-2025
ACCESSION   BBF10K_003393
VERSION     BBF10K_003393.1  
FEATURES             Location/Qualifiers
     misc_feature    5..156
                     /label=BT1_BCD2_pelB
     CDS             complement(1274..2133)
                     /label=AmpR
     promoter        complement(2135..2238)
                     /label=AmpR promoter
     primer_bind     2257..2273
                     /label=M13 fwd
     primer_bind     complement(412..427)
                     /label=M13 rev
     rep_origin      complement(495..1082)
                     /label=ColE1
     primer_bind     329..347
                     /label=SLiCE-for
     primer_bind     complement(2301..2318)
                     /label=SLiCE-rev
     misc_feature    1086..1275
                     /label=spacer
     CDS             446..496
                     /label=spacer
     terminator      362..409
                     /label=T7 terminator
     terminator      2274..2305
                     /label=tonB terminator
     misc_feature    324..2324
                     /label=pOpen_v3 backbone
     misc_feature    2420..2425
                     /label=BsaI_site
     misc_feature    complement(162..167)
                     /label=BsaI_site
     misc_feature    1..4
                     /label=TACT
     misc_feature    157..160
                     /label=CCAT
ORIGIN
        1 TACTGGGCCC AAGTTCACTT AAAAAGGAGA TCAACAATGA AAGCAATTTT CGTACTGAAA
       61 CATCTTAATC ATGCTAAGGA GGTTTTCTAA TGAAATACCT GCTGCCGACC GCTGCTGCTG
      121 GTCTGCTGCT CCTCGCTGCC CAGCCGGCGA TGGCCTCCAT CGAGACCCGG ACTATTCATT
      181 GTGGTGGTGA CGCTCGGATT ACACGGGAAA GGTGCTTGTG TCCCGACAGG CTAGGATATA
      241 ATAGGTCTTG TGCGTGACTA TCTACTGGCG GTAACGCCTG TTACTAGGGA TAACAGGGTA
      301 ATAGGAAAAT AAATCTTATC CCCCGCTGAG GTGTCAATCG TCGGAGCCGC TGAGCAATAA
      361 CTAGCATAAC CCCTTGGGGC CTCTAAACGG GTCTTGAGGG GTTTTTTGCA TGGTCATAGC
      421 TGTTTCCTGA GAGCTTGGCA GGTGATGACA CACATTAACA AATTTCGTGA GGAGTCTCCA
      481 GAAGAATGCC ATTAATTTCC ATAGGCTCCG CCCCCCTGAC GAGCATCACA AAAATCGACG
      541 CTCAAGTCAG AGGTGGCGAA ACCCGACAGG ACTATAAAGA TACCAGGCGT TTCCCCCTGG
      601 AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC CCTGCCGCTT ACCGGATACC TGTCCGCCTT
      661 TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA TAGCTCACGC TGTAGGTATC TCAGTTCGGT
      721 GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT GCACGAACCC CCCGTTCAGC CCGACCGCTG
      781 CGCCTTATCC GGTAACTATC GTCTTGAGTC CAACCCGGTA AGACACGACT TATCGCCACT
      841 GGCAGCAGCC ACTGGTAACA GGATTAGCAG AGCGAGGTAT GTAGGCGGTG CTACAGAGTT
      901 CTTGAAGTGG TGGCCTAACT ACGGCTACAC TAGAAGAACA GTATTTGGTA TCTGCGCTCT
      961 GCTGAAGCCA GTTACCTTCG GAAAAAGAGT TGGTAGCTCT TGATCCGGCA AACAAACCAC
     1021 CGCTGGTAGC GGTGGTTTTT TTGTTTGCAA GCAGCAGATT ACGCGCAGAA AAAAAGGATC
     1081 TCAAGAAGGC CTACTATTAG CAACAACGAT CCTTTGATCT TTTCTACGGG GTCTGACGCT
     1141 CAGTGGAACG AAAACTCACG TTAAGGGATT TTGGTCATGA GATTATCAAA AAGGATCTTC
     1201 ACCTAGATCC TTTTAAATTA AAAATGAAGT TTTAAATCAA TCTAAAGTAT ATATGAGTAA
     1261 ACTTGGTCTG ACAGTTACCA ATGCTTAATC AGTGAGGCAC CTATCTCAGC GATCTGTCTA
     1321 TTTCGTTCAT CCATAGTTGC CTGACTCCCC GTCGTGTAGA TAACTACGAT ACGGGAGGGC
     1381 TTACCATCTG GCCCCAGTGC TGCAATGATA CCGCGAGAAC CACGCTCACC GGCTCCAGAT
     1441 TTATCAGCAA TAAACCAGCC AGCCGGAAGG GCCGAGCGCA GAAGTGGTCC TGCAACTTTA
     1501 TCCGCCTCCA TCCAGTCTAT TAATTGTTGC CGGGAAGCTA GAGTAAGTAG TTCGCCAGTT
     1561 AATAGTTTGC GCAACGTTGT TGCCATTGCT ACAGGCATCG TGGTGTCACG CTCGTCGTTT
     1621 GGTATGGCTT CATTCAGCTC CGGTTCCCAA CGATCAAGGC GAGTTACATG ATCCCCCATG
     1681 TTGTGCAAAA AAGCGGTTAG CTCCTTCGGT CCTCCGATCG TTGTCAGAAG TAAGTTGGCC
     1741 GCAGTGTTAT CACTCATGGT TATGGCAGCA CTGCATAATT CTCTTACTGT CATGCCATCC
     1801 GTAAGATGCT TTTCTGTGAC TGGTGAGTAC TCAACCAAGT CATTCTGAGA ATAGTGTATG
     1861 CGGCGACCGA GTTGCTCTTG CCCGGCGTCA ATACGGGATA ATACCGCGCC ACATAGCAGA
     1921 ACTTTAAAAG TGCTCATCAT TGGAAAACGT TCTTCGGGGC GAAAACTCTC AAGGATCTTA
     1981 CCGCTGTTGA GATCCAGTTC GATGTAACCC ACTCGTGCAC CCAACTGATC TTCAGCATCT
     2041 TTTACTTTCA CCAGCGTTTC TGGGTGAGCA AAAACAGGAA GGCAAAATGC CGCAAAAAAG
     2101 GGAATAAGGG CGACACGGAA ATGTTGAATA CTCATACTCT TCCTTTTTCA ATATTATTGA
     2161 AGCATTTATC AGGGTTATTG TCTCATGAGC GGATACATAT TTGAATGTAT TTAGAAAAAT
     2221 AAACAAATAG GGGTTCCGCG CACCTGCACC AGTCAGTAAA ACGACGGCCA GTAGTCAAAA
     2281 GCCTCCGACC GGAGGCTTTT GACTTGGTTC AGGTGGAGTG GGAGAACGAT GATGCTCACT
     2341 CTCGGCTGAG GCGTTACCCC AATCGTTCAG CGTGGGATTT GCTACAACTT CTGAGTGCTA
     2401 CATGTACCAG ACCATGTTAG GTCTCA
//