LOCUS       BBF10K_003395           2405 bp    DNA     circular     29-Apr-2025
ACCESSION   BBF10K_003395
VERSION     BBF10K_003395.1  
FEATURES             Location/Qualifiers
     CDS             5..145
                     /label=T1T2_Calm
     CDS             complement(1258..2117)
                     /label=AmpR
     promoter        complement(2119..2222)
                     /label=AmpR promoter
     primer_bind     2241..2257
                     /label=M13 fwd
     primer_bind     complement(396..411)
                     /label=M13 rev
     rep_origin      complement(479..1066)
                     /label=ColE1
     primer_bind     313..331
                     /label=SLiCE-for
     primer_bind     complement(2285..2302)
                     /label=SLiCE-rev
     misc_feature    1070..1259
                     /label=spacer
     CDS             430..480
                     /label=spacer
     terminator      346..393
                     /label=T7 terminator
     terminator      2258..2289
                     /label=tonB terminator
     misc_feature    308..2308
                     /label=pOpen_v3 backbone
     misc_feature    2399..2404
                     /label=BsaI_site
     misc_feature    complement(151..156)
                     /label=BsaI_site
     misc_feature    1..4
                     /label=CCAT
     misc_feature    146..149
                     /label=GTCA
ORIGIN
        1 CCATGGGCGT GATGCCGCGC GAAGAAACCG ATAGCAAAAC CGCGAGCCCG TGGAAAAGCG
       61 CGCGCCTGAT GGTGCATACC GTGGCGACCT TTAACAGCAT TAAAGAACTG AACGAACGCT
      121 GGCGCAGCCT GCAGCAGCTG GCGGGGTCAC GAGACCCTGG TACAACAGGA CTATGAAACC
      181 GGTACAAAGG CAACATCCTC ACTTAGGTGA ATCGAAACGC GGTATCACCA GTATCGGCAG
      241 CTAATCTAGT GCCAGCGGTA GGATGGCCTA GGGATAACAG GGTAATATGT ATGATTTTCA
      301 TGTAGCACGC TGAGGTGTCA ATCGTCGGAG CCGCTGAGCA ATAACTAGCA TAACCCCTTG
      361 GGGCCTCTAA ACGGGTCTTG AGGGGTTTTT TGCATGGTCA TAGCTGTTTC CTGAGAGCTT
      421 GGCAGGTGAT GACACACATT AACAAATTTC GTGAGGAGTC TCCAGAAGAA TGCCATTAAT
      481 TTCCATAGGC TCCGCCCCCC TGACGAGCAT CACAAAAATC GACGCTCAAG TCAGAGGTGG
      541 CGAAACCCGA CAGGACTATA AAGATACCAG GCGTTTCCCC CTGGAAGCTC CCTCGTGCGC
      601 TCTCCTGTTC CGACCCTGCC GCTTACCGGA TACCTGTCCG CCTTTCTCCC TTCGGGAAGC
      661 GTGGCGCTTT CTCATAGCTC ACGCTGTAGG TATCTCAGTT CGGTGTAGGT CGTTCGCTCC
      721 AAGCTGGGCT GTGTGCACGA ACCCCCCGTT CAGCCCGACC GCTGCGCCTT ATCCGGTAAC
      781 TATCGTCTTG AGTCCAACCC GGTAAGACAC GACTTATCGC CACTGGCAGC AGCCACTGGT
      841 AACAGGATTA GCAGAGCGAG GTATGTAGGC GGTGCTACAG AGTTCTTGAA GTGGTGGCCT
      901 AACTACGGCT ACACTAGAAG AACAGTATTT GGTATCTGCG CTCTGCTGAA GCCAGTTACC
      961 TTCGGAAAAA GAGTTGGTAG CTCTTGATCC GGCAAACAAA CCACCGCTGG TAGCGGTGGT
     1021 TTTTTTGTTT GCAAGCAGCA GATTACGCGC AGAAAAAAAG GATCTCAAGA AGGCCTACTA
     1081 TTAGCAACAA CGATCCTTTG ATCTTTTCTA CGGGGTCTGA CGCTCAGTGG AACGAAAACT
     1141 CACGTTAAGG GATTTTGGTC ATGAGATTAT CAAAAAGGAT CTTCACCTAG ATCCTTTTAA
     1201 ATTAAAAATG AAGTTTTAAA TCAATCTAAA GTATATATGA GTAAACTTGG TCTGACAGTT
     1261 ACCAATGCTT AATCAGTGAG GCACCTATCT CAGCGATCTG TCTATTTCGT TCATCCATAG
     1321 TTGCCTGACT CCCCGTCGTG TAGATAACTA CGATACGGGA GGGCTTACCA TCTGGCCCCA
     1381 GTGCTGCAAT GATACCGCGA GAACCACGCT CACCGGCTCC AGATTTATCA GCAATAAACC
     1441 AGCCAGCCGG AAGGGCCGAG CGCAGAAGTG GTCCTGCAAC TTTATCCGCC TCCATCCAGT
     1501 CTATTAATTG TTGCCGGGAA GCTAGAGTAA GTAGTTCGCC AGTTAATAGT TTGCGCAACG
     1561 TTGTTGCCAT TGCTACAGGC ATCGTGGTGT CACGCTCGTC GTTTGGTATG GCTTCATTCA
     1621 GCTCCGGTTC CCAACGATCA AGGCGAGTTA CATGATCCCC CATGTTGTGC AAAAAAGCGG
     1681 TTAGCTCCTT CGGTCCTCCG ATCGTTGTCA GAAGTAAGTT GGCCGCAGTG TTATCACTCA
     1741 TGGTTATGGC AGCACTGCAT AATTCTCTTA CTGTCATGCC ATCCGTAAGA TGCTTTTCTG
     1801 TGACTGGTGA GTACTCAACC AAGTCATTCT GAGAATAGTG TATGCGGCGA CCGAGTTGCT
     1861 CTTGCCCGGC GTCAATACGG GATAATACCG CGCCACATAG CAGAACTTTA AAAGTGCTCA
     1921 TCATTGGAAA ACGTTCTTCG GGGCGAAAAC TCTCAAGGAT CTTACCGCTG TTGAGATCCA
     1981 GTTCGATGTA ACCCACTCGT GCACCCAACT GATCTTCAGC ATCTTTTACT TTCACCAGCG
     2041 TTTCTGGGTG AGCAAAAACA GGAAGGCAAA ATGCCGCAAA AAAGGGAATA AGGGCGACAC
     2101 GGAAATGTTG AATACTCATA CTCTTCCTTT TTCAATATTA TTGAAGCATT TATCAGGGTT
     2161 ATTGTCTCAT GAGCGGATAC ATATTTGAAT GTATTTAGAA AAATAAACAA ATAGGGGTTC
     2221 CGCGCACCTG CACCAGTCAG TAAAACGACG GCCAGTAGTC AAAAGCCTCC GACCGGAGGC
     2281 TTTTGACTTG GTTCAGGTGG AGTGGGAGAA CGATGATGCT CACTCTCGAA GATTACTTTT
     2341 TGGATACCTG AAACAAATCC CATGGTAGTC CTTAGACTTG GGAGTCTATC ACCTCTAGGG
     2401 TCTCA
//