LOCUS BBF10K_003396 3699 bp DNA circular 29-Apr-2025 ACCESSION BBF10K_003396 VERSION BBF10K_003396.1 FEATURES Location/Qualifiers CDS 5..1579 /label=T1T2_CBD CDS complement(2622..3481) /label=AmpR promoter complement(3483..3586) /label=AmpR promoter primer_bind 3605..3621 /label=M13 fwd primer_bind complement(1760..1775) /label=M13 rev rep_origin complement(1843..2430) /label=ColE1 primer_bind 1677..1695 /label=SLiCE-for primer_bind complement(3649..3666) /label=SLiCE-rev misc_feature 2434..2623 /label=spacer CDS 1794..1844 /label=spacer terminator 1710..1757 /label=T7 terminator terminator 3622..3653 /label=tonB terminator misc_feature 1672..3672 /label=pOpen_v3 backbone misc_feature 3693..3698 /label=BsaI_site misc_feature complement(1585..1590) /label=BsaI_site misc_feature 1..4 /label=CCAT misc_feature 1580..1583 /label=GTCA ORIGIN 1 CCATGAAGAT CGAAGAAGGG AAGTTAGTGA TCGGATCGTT AGAAGGCTGC TTTGCGAAAG 61 GAACCAATGT CCTGATGGCG GACGGTAGCA TTGAATGTAT CGAAAACATC GAAGTCGGAA 121 ACAAGGTGAT GGGGAAAGAT GGTCGTCCGC GTGAAGTTAT CAAACTGCCC CGTGGACGCG 181 AAACTATGTA CTCAGTTGTC CAAAAGTCCC AGCACCGTGC GCACAAAAGT GATTCGTCGC 241 GTGAAGTGCC CGAGCTGTTG AAGTTCACCT GTAACGCAAC GCACGAACTG GTAGTGCGTA 301 CACCTCGCTC TGTGCGTCGT CTGTCTCGTA CCATTAAGGG TGTCGAGTAT TTCGAGGTGA 361 TCACTTTCGA AATGGGTCAG AAGAAGGCAC CCGACGGTCG TATTGTGGAA CTGGTGAAAG 421 AGGTGTCAAA GTCATACCCG ATCAGCGAAG GTCCGGAACG TGCCAATGAG TTAGTTGAAT 481 CGTACCGCAA AGCTTCTAAC AAAGCATACT TCGAATGGAC CATCGAAGCG CGTGACCTGT 541 CTCTGCTGGG GAGCCATGTG CGTAAAGCAA CCTATCAGAC CTACGCTCCG ATCCTGTACG 601 AGAACGACCA CTTCTTTGAT TACATGCAGA AATCGAAATT CCACCTGACC ATCGAGGGGC 661 CAAAAGTCCT GGCCTACTTA CTGGGTCTTT GGATTGGTGA TGGTCTTAGC GATCGTGCAA 721 CCTTCTCAGT AGATAGTCGT GATACCTCTC TGATGGAACG TGTGACTGAG TATGCGGAAA 781 AGCTGAATCT GTGTGCCGAG TACAAGGACC GTAAGGAACC GCAAGTCGCG AAAACCGTTA 841 ACCTGTATTC AAAAGTTGTT CGTGGTGCAT CTACGAACCC GGGAGTTAGT GCGTGGCAAG 901 TCAACACCGC TTATACGGCT GGCCAACTGG TAACCTATAA CGGGAAAACG TACAAGTGCC 961 TGCAACCGCA CACCTCCTTG GCGGGATGGG AGCCGAGCAA CGTGCCAGCG CTGTGGCAAT 1021 TGCAGGGCGG TCACGGCGGT ATCCGCAACA ATTTGAATAC GGAAAACCCC TTGTGGGACG 1081 CGATCGTGGG CCTGGGTTTC CTGAAAGACG GGGTCAAGAA CATCCCATCC TTCCTGAGCA 1141 CCGATAACAT TGGTACGCGT GAGACATTCC TGGCAGGATT GATTGATTCG GATGGCTATG 1201 TGACTGACGA ACACGGTATT AAGGCTACTA TCAAAACCAT TCATACCTCT GTTCGCGATG 1261 GGCTGGTTTC TTTAGCTCGC TCGCTGGGTT TAGTGGTGAG CGTTAACGCG GAACCGGCAA 1321 AAGTCGATAT GAATGTGACC AAACACAAGA TCTCTTATGC GATTTACATG TCTGGCGGAG 1381 ATGTTCTGCT TAACGTTCTG TCTAAATGTG CGGGTAGCAA GAAATTCCGT CCTGCGCCAG 1441 CGGCAGCTTT TGCGCGTGAA TGTCGTGGTT TCTACTTCGA ATTGCAAGAA TTGAAAGAGG 1501 ACGACTATTA CGGCATTACC CTGTCCGACG ACAGCGATCA TCAATTCCTG TTGGGTTCTC 1561 AAGTGGTCGT ACAAAACGGG TCACGAGACC AGTCCGTCGG AGTAACAATC TTTCATGAAG 1621 CTACACAAGC GCTAGGGATA ACAGGGTAAT TGATCTCAGG GCCTCTAAAC ACGCTGAGGT 1681 GTCAATCGTC GGAGCCGCTG AGCAATAACT AGCATAACCC CTTGGGGCCT CTAAACGGGT 1741 CTTGAGGGGT TTTTTGCATG GTCATAGCTG TTTCCTGAGA GCTTGGCAGG TGATGACACA 1801 CATTAACAAA TTTCGTGAGG AGTCTCCAGA AGAATGCCAT TAATTTCCAT AGGCTCCGCC 1861 CCCCTGACGA GCATCACAAA AATCGACGCT CAAGTCAGAG GTGGCGAAAC CCGACAGGAC 1921 TATAAAGATA CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT GCGCTCTCCT GTTCCGACCC 1981 TGCCGCTTAC CGGATACCTG TCCGCCTTTC TCCCTTCGGG AAGCGTGGCG CTTTCTCATA 2041 GCTCACGCTG TAGGTATCTC AGTTCGGTGT AGGTCGTTCG CTCCAAGCTG GGCTGTGTGC 2101 ACGAACCCCC CGTTCAGCCC GACCGCTGCG CCTTATCCGG TAACTATCGT CTTGAGTCCA 2161 ACCCGGTAAG ACACGACTTA TCGCCACTGG CAGCAGCCAC TGGTAACAGG ATTAGCAGAG 2221 CGAGGTATGT AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG GCCTAACTAC GGCTACACTA 2281 GAAGAACAGT ATTTGGTATC TGCGCTCTGC TGAAGCCAGT TACCTTCGGA AAAAGAGTTG 2341 GTAGCTCTTG ATCCGGCAAA CAAACCACCG CTGGTAGCGG TGGTTTTTTT GTTTGCAAGC 2401 AGCAGATTAC GCGCAGAAAA AAAGGATCTC AAGAAGGCCT ACTATTAGCA ACAACGATCC 2461 TTTGATCTTT TCTACGGGGT CTGACGCTCA GTGGAACGAA AACTCACGTT AAGGGATTTT 2521 GGTCATGAGA TTATCAAAAA GGATCTTCAC CTAGATCCTT TTAAATTAAA AATGAAGTTT 2581 TAAATCAATC TAAAGTATAT ATGAGTAAAC TTGGTCTGAC AGTTACCAAT GCTTAATCAG 2641 TGAGGCACCT ATCTCAGCGA TCTGTCTATT TCGTTCATCC ATAGTTGCCT GACTCCCCGT 2701 CGTGTAGATA ACTACGATAC GGGAGGGCTT ACCATCTGGC CCCAGTGCTG CAATGATACC 2761 GCGAGAACCA CGCTCACCGG CTCCAGATTT ATCAGCAATA AACCAGCCAG CCGGAAGGGC 2821 CGAGCGCAGA AGTGGTCCTG CAACTTTATC CGCCTCCATC CAGTCTATTA ATTGTTGCCG 2881 GGAAGCTAGA GTAAGTAGTT CGCCAGTTAA TAGTTTGCGC AACGTTGTTG CCATTGCTAC 2941 AGGCATCGTG GTGTCACGCT CGTCGTTTGG TATGGCTTCA TTCAGCTCCG GTTCCCAACG 3001 ATCAAGGCGA GTTACATGAT CCCCCATGTT GTGCAAAAAA GCGGTTAGCT CCTTCGGTCC 3061 TCCGATCGTT GTCAGAAGTA AGTTGGCCGC AGTGTTATCA CTCATGGTTA TGGCAGCACT 3121 GCATAATTCT CTTACTGTCA TGCCATCCGT AAGATGCTTT TCTGTGACTG GTGAGTACTC 3181 AACCAAGTCA TTCTGAGAAT AGTGTATGCG GCGACCGAGT TGCTCTTGCC CGGCGTCAAT 3241 ACGGGATAAT ACCGCGCCAC ATAGCAGAAC TTTAAAAGTG CTCATCATTG GAAAACGTTC 3301 TTCGGGGCGA AAACTCTCAA GGATCTTACC GCTGTTGAGA TCCAGTTCGA TGTAACCCAC 3361 TCGTGCACCC AACTGATCTT CAGCATCTTT TACTTTCACC AGCGTTTCTG GGTGAGCAAA 3421 AACAGGAAGG CAAAATGCCG CAAAAAAGGG AATAAGGGCG ACACGGAAAT GTTGAATACT 3481 CATACTCTTC CTTTTTCAAT ATTATTGAAG CATTTATCAG GGTTATTGTC TCATGAGCGG 3541 ATACATATTT GAATGTATTT AGAAAAATAA ACAAATAGGG GTTCCGCGCA CCTGCACCAG 3601 TCAGTAAAAC GACGGCCAGT AGTCAAAAGC CTCCGACCGG AGGCTTTTGA CTTGGTTCAG 3661 GTGGAGTGGG AGAACGATGA TGCTCACTCT CGGGTCTCA //