LOCUS       BBF10K_003397           2445 bp    DNA     circular     29-Apr-2025
ACCESSION   BBF10K_003397
VERSION     BBF10K_003397.1  
FEATURES             Location/Qualifiers
     CDS             5..325
                     /label=T1T2_Cex
     CDS             complement(1368..2227)
                     /label=AmpR
     promoter        complement(2229..2332)
                     /label=AmpR promoter
     primer_bind     2351..2367
                     /label=M13 fwd
     primer_bind     complement(506..521)
                     /label=M13 rev
     rep_origin      complement(589..1176)
                     /label=ColE1
     primer_bind     423..441
                     /label=SLiCE-for
     primer_bind     complement(2395..2412)
                     /label=SLiCE-rev
     misc_feature    1180..1369
                     /label=spacer
     CDS             540..590
                     /label=spacer
     terminator      456..503
                     /label=T7 terminator
     terminator      2368..2399
                     /label=tonB terminator
     misc_feature    418..2418
                     /label=pOpen_v3 backbone
     misc_feature    2439..2444
                     /label=BsaI_site
     misc_feature    complement(331..336)
                     /label=BsaI_site
     misc_feature    1..4
                     /label=CCAT
     misc_feature    326..329
                     /label=GTCA
ORIGIN
        1 CCATGCCTGC AGGTTGTCAG GTGCTGTGGG GCGTCAACCA GTGGAATACT GGCTTTACCG
       61 CAAACGTCAC GGTGAAAAAC ACCTCGTCTG CTCCGGTTGA TGGCTGGACA TTAACTTTCT
      121 CGTTTCCGAG CGGTCAGCAA GTGACGCAGG CGTGGTCCAG TACCGTTACG CAATCCGGAA
      181 GCGCGGTAAC CGTACGCAAC GCCCCGTGGA ATGGCAGTAT TCCAGCTGGG GGCACTGCGC
      241 AATTTGGTTT CAATGGTAGC CATACCGGTA CCAATGCGGC CCCCACAGCC TTCTCACTGA
      301 ACGGGACCCC GTGCACGGTG GGAGGGTCAC GAGACCAGGG AATCTCGTCG GAAATCTATT
      361 TTGGCACTCC ACTTTAAGTA GGGATAACAG GGTAATCACG CAAAAATAAG TTCAATACGC
      421 TGAGGTGTCA ATCGTCGGAG CCGCTGAGCA ATAACTAGCA TAACCCCTTG GGGCCTCTAA
      481 ACGGGTCTTG AGGGGTTTTT TGCATGGTCA TAGCTGTTTC CTGAGAGCTT GGCAGGTGAT
      541 GACACACATT AACAAATTTC GTGAGGAGTC TCCAGAAGAA TGCCATTAAT TTCCATAGGC
      601 TCCGCCCCCC TGACGAGCAT CACAAAAATC GACGCTCAAG TCAGAGGTGG CGAAACCCGA
      661 CAGGACTATA AAGATACCAG GCGTTTCCCC CTGGAAGCTC CCTCGTGCGC TCTCCTGTTC
      721 CGACCCTGCC GCTTACCGGA TACCTGTCCG CCTTTCTCCC TTCGGGAAGC GTGGCGCTTT
      781 CTCATAGCTC ACGCTGTAGG TATCTCAGTT CGGTGTAGGT CGTTCGCTCC AAGCTGGGCT
      841 GTGTGCACGA ACCCCCCGTT CAGCCCGACC GCTGCGCCTT ATCCGGTAAC TATCGTCTTG
      901 AGTCCAACCC GGTAAGACAC GACTTATCGC CACTGGCAGC AGCCACTGGT AACAGGATTA
      961 GCAGAGCGAG GTATGTAGGC GGTGCTACAG AGTTCTTGAA GTGGTGGCCT AACTACGGCT
     1021 ACACTAGAAG AACAGTATTT GGTATCTGCG CTCTGCTGAA GCCAGTTACC TTCGGAAAAA
     1081 GAGTTGGTAG CTCTTGATCC GGCAAACAAA CCACCGCTGG TAGCGGTGGT TTTTTTGTTT
     1141 GCAAGCAGCA GATTACGCGC AGAAAAAAAG GATCTCAAGA AGGCCTACTA TTAGCAACAA
     1201 CGATCCTTTG ATCTTTTCTA CGGGGTCTGA CGCTCAGTGG AACGAAAACT CACGTTAAGG
     1261 GATTTTGGTC ATGAGATTAT CAAAAAGGAT CTTCACCTAG ATCCTTTTAA ATTAAAAATG
     1321 AAGTTTTAAA TCAATCTAAA GTATATATGA GTAAACTTGG TCTGACAGTT ACCAATGCTT
     1381 AATCAGTGAG GCACCTATCT CAGCGATCTG TCTATTTCGT TCATCCATAG TTGCCTGACT
     1441 CCCCGTCGTG TAGATAACTA CGATACGGGA GGGCTTACCA TCTGGCCCCA GTGCTGCAAT
     1501 GATACCGCGA GAACCACGCT CACCGGCTCC AGATTTATCA GCAATAAACC AGCCAGCCGG
     1561 AAGGGCCGAG CGCAGAAGTG GTCCTGCAAC TTTATCCGCC TCCATCCAGT CTATTAATTG
     1621 TTGCCGGGAA GCTAGAGTAA GTAGTTCGCC AGTTAATAGT TTGCGCAACG TTGTTGCCAT
     1681 TGCTACAGGC ATCGTGGTGT CACGCTCGTC GTTTGGTATG GCTTCATTCA GCTCCGGTTC
     1741 CCAACGATCA AGGCGAGTTA CATGATCCCC CATGTTGTGC AAAAAAGCGG TTAGCTCCTT
     1801 CGGTCCTCCG ATCGTTGTCA GAAGTAAGTT GGCCGCAGTG TTATCACTCA TGGTTATGGC
     1861 AGCACTGCAT AATTCTCTTA CTGTCATGCC ATCCGTAAGA TGCTTTTCTG TGACTGGTGA
     1921 GTACTCAACC AAGTCATTCT GAGAATAGTG TATGCGGCGA CCGAGTTGCT CTTGCCCGGC
     1981 GTCAATACGG GATAATACCG CGCCACATAG CAGAACTTTA AAAGTGCTCA TCATTGGAAA
     2041 ACGTTCTTCG GGGCGAAAAC TCTCAAGGAT CTTACCGCTG TTGAGATCCA GTTCGATGTA
     2101 ACCCACTCGT GCACCCAACT GATCTTCAGC ATCTTTTACT TTCACCAGCG TTTCTGGGTG
     2161 AGCAAAAACA GGAAGGCAAA ATGCCGCAAA AAAGGGAATA AGGGCGACAC GGAAATGTTG
     2221 AATACTCATA CTCTTCCTTT TTCAATATTA TTGAAGCATT TATCAGGGTT ATTGTCTCAT
     2281 GAGCGGATAC ATATTTGAAT GTATTTAGAA AAATAAACAA ATAGGGGTTC CGCGCACCTG
     2341 CACCAGTCAG TAAAACGACG GCCAGTAGTC AAAAGCCTCC GACCGGAGGC TTTTGACTTG
     2401 GTTCAGGTGG AGTGGGAGAA CGATGATGCT CACTCTCGGG TCTCA
//