LOCUS BBF10K_003398 3225 bp DNA circular 29-Apr-2025 ACCESSION BBF10K_003398 VERSION BBF10K_003398.1 FEATURES Location/Qualifiers CDS 5..1105 /label=T1T2_MBP CDS complement(2148..3007) /label=AmpR promoter complement(3009..3112) /label=AmpR promoter primer_bind 3131..3147 /label=M13 fwd primer_bind complement(1286..1301) /label=M13 rev rep_origin complement(1369..1956) /label=ColE1 primer_bind 1203..1221 /label=SLiCE-for primer_bind complement(3175..3192) /label=SLiCE-rev misc_feature 1960..2149 /label=spacer CDS 1320..1370 /label=spacer terminator 1236..1283 /label=T7 terminator terminator 3148..3179 /label=tonB terminator misc_feature 1198..3198 /label=pOpen_v3 backbone misc_feature 3219..3224 /label=BsaI_site misc_feature complement(1111..1116) /label=BsaI_site misc_feature 1..4 /label=CCAT misc_feature 1106..1109 /label=GTCA ORIGIN 1 CCATGAAAAT CGAAGAAGGT AAACTGGTAA TCTGGATTAA CGGCGATAAA GGCTATAACG 61 GCCTCGCTGA AGTCGGTAAG AAATTCGAGA AAGATACCGG AATTAAAGTC ACCGTTGAGC 121 ATCCGGATAA ACTGGAAGAG AAATTCCCAC AGGTTGCGGC AACTGGCGAT GGCCCTGACA 181 TTATCTTCTG GGCACACGAC CGCTTTGGTG GCTACGCTCA ATCTGGCCTG TTGGCTGAAA 241 TCACCCCGGA CAAAGCGTTC CAGGACAAGC TGTATCCGTT TACCTGGGAT GCCGTACGTT 301 ACAACGGCAA GCTGATTGCT TACCCGATCG CTGTTGAAGC GTTATCGCTG ATTTATAACA 361 AAGACCTGCT GCCGAACCCG CCAAAAACCT GGGAAGAGAT CCCGGCGCTG GATAAAGAAC 421 TGAAAGCGAA AGGTAAGAGC GCGCTGATGT TCAACCTGCA AGAACCGTAC TTCACCTGGC 481 CGCTGATTGC TGCTGACGGG GGTTATGCGT TCAAGTATGA AAACGGCAAG TACGACATTA 541 AAGACGTGGG CGTGGATAAC GCTGGCGCGA AAGCGGGTCT GACCTTCCTG GTTGACCTGA 601 TTAAAAACAA ACACATGAAT GCAGACACCG ATTACTCCAT CGCAGAAGCT GCCTTTAATA 661 AAGGCGAAAC AGCGATGACC ATCAACGGCC CGTGGGCATG GTCCAACATC GACACCAGCA 721 AAGTGAATTA TGGTGTAACG GTACTGCCGA CCTTCAAGGG TCAACCATCC AAACCGTTCG 781 TTGGCGTGCT GAGCGCAGGT ATTAACGCCG CCAGTCCGAA CAAAGAGCTG GCGAAAGAGT 841 TCCTCGAAAA CTATCTGCTG ACTGATGAAG GTCTGGAAGC GGTTAATAAA GACAAACCGC 901 TGGGTGCCGT AGCGCTGAAG TCTTACGAGG AAGAGTTGGC GAAAGATCCA CGTATTGCCG 961 CCACGATGGA AAACGCCCAG AAAGGTGAAA TCATGCCGAA CATCCCGCAG ATGTCCGCTT 1021 TCTGGTATGC CGTGCGTACT GCGGTGATCA ACGCCGCCAG CGGTCGTCAG ACTGTCGATG 1081 AAGCCCTGAA AGACGCGCAG ACTGGGTCAC GAGACCACCG AGATTTAGAC GGATGCTTTC 1141 GAATCTCATA GAGAGGAGTA GGGATAACAG GGTAATTCGT TTACTAACTT AAAGTTGCGC 1201 TGAGGTGTCA ATCGTCGGAG CCGCTGAGCA ATAACTAGCA TAACCCCTTG GGGCCTCTAA 1261 ACGGGTCTTG AGGGGTTTTT TGCATGGTCA TAGCTGTTTC CTGAGAGCTT GGCAGGTGAT 1321 GACACACATT AACAAATTTC GTGAGGAGTC TCCAGAAGAA TGCCATTAAT TTCCATAGGC 1381 TCCGCCCCCC TGACGAGCAT CACAAAAATC GACGCTCAAG TCAGAGGTGG CGAAACCCGA 1441 CAGGACTATA AAGATACCAG GCGTTTCCCC CTGGAAGCTC CCTCGTGCGC TCTCCTGTTC 1501 CGACCCTGCC GCTTACCGGA TACCTGTCCG CCTTTCTCCC TTCGGGAAGC GTGGCGCTTT 1561 CTCATAGCTC ACGCTGTAGG TATCTCAGTT CGGTGTAGGT CGTTCGCTCC AAGCTGGGCT 1621 GTGTGCACGA ACCCCCCGTT CAGCCCGACC GCTGCGCCTT ATCCGGTAAC TATCGTCTTG 1681 AGTCCAACCC GGTAAGACAC GACTTATCGC CACTGGCAGC AGCCACTGGT AACAGGATTA 1741 GCAGAGCGAG GTATGTAGGC GGTGCTACAG AGTTCTTGAA GTGGTGGCCT AACTACGGCT 1801 ACACTAGAAG AACAGTATTT GGTATCTGCG CTCTGCTGAA GCCAGTTACC TTCGGAAAAA 1861 GAGTTGGTAG CTCTTGATCC GGCAAACAAA CCACCGCTGG TAGCGGTGGT TTTTTTGTTT 1921 GCAAGCAGCA GATTACGCGC AGAAAAAAAG GATCTCAAGA AGGCCTACTA TTAGCAACAA 1981 CGATCCTTTG ATCTTTTCTA CGGGGTCTGA CGCTCAGTGG AACGAAAACT CACGTTAAGG 2041 GATTTTGGTC ATGAGATTAT CAAAAAGGAT CTTCACCTAG ATCCTTTTAA ATTAAAAATG 2101 AAGTTTTAAA TCAATCTAAA GTATATATGA GTAAACTTGG TCTGACAGTT ACCAATGCTT 2161 AATCAGTGAG GCACCTATCT CAGCGATCTG TCTATTTCGT TCATCCATAG TTGCCTGACT 2221 CCCCGTCGTG TAGATAACTA CGATACGGGA GGGCTTACCA TCTGGCCCCA GTGCTGCAAT 2281 GATACCGCGA GAACCACGCT CACCGGCTCC AGATTTATCA GCAATAAACC AGCCAGCCGG 2341 AAGGGCCGAG CGCAGAAGTG GTCCTGCAAC TTTATCCGCC TCCATCCAGT CTATTAATTG 2401 TTGCCGGGAA GCTAGAGTAA GTAGTTCGCC AGTTAATAGT TTGCGCAACG TTGTTGCCAT 2461 TGCTACAGGC ATCGTGGTGT CACGCTCGTC GTTTGGTATG GCTTCATTCA GCTCCGGTTC 2521 CCAACGATCA AGGCGAGTTA CATGATCCCC CATGTTGTGC AAAAAAGCGG TTAGCTCCTT 2581 CGGTCCTCCG ATCGTTGTCA GAAGTAAGTT GGCCGCAGTG TTATCACTCA TGGTTATGGC 2641 AGCACTGCAT AATTCTCTTA CTGTCATGCC ATCCGTAAGA TGCTTTTCTG TGACTGGTGA 2701 GTACTCAACC AAGTCATTCT GAGAATAGTG TATGCGGCGA CCGAGTTGCT CTTGCCCGGC 2761 GTCAATACGG GATAATACCG CGCCACATAG CAGAACTTTA AAAGTGCTCA TCATTGGAAA 2821 ACGTTCTTCG GGGCGAAAAC TCTCAAGGAT CTTACCGCTG TTGAGATCCA GTTCGATGTA 2881 ACCCACTCGT GCACCCAACT GATCTTCAGC ATCTTTTACT TTCACCAGCG TTTCTGGGTG 2941 AGCAAAAACA GGAAGGCAAA ATGCCGCAAA AAAGGGAATA AGGGCGACAC GGAAATGTTG 3001 AATACTCATA CTCTTCCTTT TTCAATATTA TTGAAGCATT TATCAGGGTT ATTGTCTCAT 3061 GAGCGGATAC ATATTTGAAT GTATTTAGAA AAATAAACAA ATAGGGGTTC CGCGCACCTG 3121 CACCAGTCAG TAAAACGACG GCCAGTAGTC AAAAGCCTCC GACCGGAGGC TTTTGACTTG 3181 GTTCAGGTGG AGTGGGAGAA CGATGATGCT CACTCTCGGG TCTCA //