LOCUS       BBF10K_003400           2778 bp    DNA     circular     17-Jun-2025
ACCESSION   BBF10K_003400
VERSION     BBF10K_003400.1  
FEATURES             Location/Qualifiers
     CDS             5..658
                     /label=T1T2_GST
     CDS             complement(1701..2560)
                     /label=AmpR
     promoter        complement(2562..2665)
                     /label=AmpR promoter
     primer_bind     2684..2700
                     /label=M13 fwd
     primer_bind     complement(839..854)
                     /label=M13 rev
     rep_origin      complement(922..1509)
                     /label=ColE1
     primer_bind     756..774
                     /label=SLiCE-for
     primer_bind     complement(2728..2745)
                     /label=SLiCE-rev
     misc_feature    1513..1702
                     /label=spacer
     CDS             873..923
                     /label=spacer
     terminator      789..836
                     /label=T7 terminator
     terminator      2701..2732
                     /label=tonB terminator
     misc_feature    751..2751
                     /label=pOpen_v3 backbone
     misc_feature    2772..2777
                     /label=BsaI_site
     misc_feature    complement(664..669)
                     /label=BsaI_site
     misc_feature    1..4
                     /label=CCAT
     misc_feature    659..662
                     /label=GTCA
ORIGIN
        1 CCATGTCCCC TATACTAGGT TATTGGAAAA TTAAGGGCCT TGTGCAACCC ACTCGACTTC
       61 TTTTGGAATA TCTTGAAGAA AAATATGAGG AGCATTTGTA TGAGCGCGAT GAAGGTGATA
      121 AATGGCGAAA CAAAAAGTTT GAATTGGGTT TGGAGTTTCC CAATCTTCCT TATTATATTG
      181 ATGGTGATGT TAAATTAACA CAGTCTATGG CCATCATACG TTATATAGCT GACAAGCACA
      241 ACATGTTGGG TGGTTGTCCA AAAGAGCGTG CAGAGATTTC AATGCTTGAA GGAGCGGTTT
      301 TGGATATTAG ATACGGTGTT TCGAGAATTG CATATAGTAA AGACTTTGAA ACTCTCAAAG
      361 TTGATTTTCT TAGCAAGCTA CCTGAAATGC TGAAAATGTT CGAAGATCGT TTATGTCATA
      421 AAACATATCT GAATGGTGAT CATGTAACCC ATCCTGACTT CATGTTGTAT GACGCTCTTG
      481 ATGTTGTTTT ATACATGGAC CCAATGTGCC TGGATGCGTT CCCAAAATTA GTTTGTTTTA
      541 AAAAACGTAT TGAAGCTATC CCACAAATTG ATAAGTACTT GAAATCCAGC AAGTATATAG
      601 CATGGCCTTT GCAGGGCTGG CAAGCCACGT TTGGTGGTGG CGACCATCCT CCAAAAGGGT
      661 CACGAGACCA GTTACACCGG GATCATAGCT TATCGGAACG CAACGCGTCG CTAGGGATAA
      721 CAGGGTAATA CCTATCGCTC TGGCCCTCAG CGCTGAGGTG TCAATCGTCG GAGCCGCTGA
      781 GCAATAACTA GCATAACCCC TTGGGGCCTC TAAACGGGTC TTGAGGGGTT TTTTGCATGG
      841 TCATAGCTGT TTCCTGAGAG CTTGGCAGGT GATGACACAC ATTAACAAAT TTCGTGAGGA
      901 GTCTCCAGAA GAATGCCATT AATTTCCATA GGCTCCGCCC CCCTGACGAG CATCACAAAA
      961 ATCGACGCTC AAGTCAGAGG TGGCGAAACC CGACAGGACT ATAAAGATAC CAGGCGTTTC
     1021 CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG TTCCGACCCT GCCGCTTACC GGATACCTGT
     1081 CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC TTTCTCATAG CTCACGCTGT AGGTATCTCA
     1141 GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG GCTGTGTGCA CGAACCCCCC GTTCAGCCCG
     1201 ACCGCTGCGC CTTATCCGGT AACTATCGTC TTGAGTCCAA CCCGGTAAGA CACGACTTAT
     1261 CGCCACTGGC AGCAGCCACT GGTAACAGGA TTAGCAGAGC GAGGTATGTA GGCGGTGCTA
     1321 CAGAGTTCTT GAAGTGGTGG CCTAACTACG GCTACACTAG AAGAACAGTA TTTGGTATCT
     1381 GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC
     1441 AAACCACCGC TGGTAGCGGT GGTTTTTTTG TTTGCAAGCA GCAGATTACG CGCAGAAAAA
     1501 AAGGATCTCA AGAAGGCCTA CTATTAGCAA CAACGATCCT TTGATCTTTT CTACGGGGTC
     1561 TGACGCTCAG TGGAACGAAA ACTCACGTTA AGGGATTTTG GTCATGAGAT TATCAAAAAG
     1621 GATCTTCACC TAGATCCTTT TAAATTAAAA ATGAAGTTTT AAATCAATCT AAAGTATATA
     1681 TGAGTAAACT TGGTCTGACA GTTACCAATG CTTAATCAGT GAGGCACCTA TCTCAGCGAT
     1741 CTGTCTATTT CGTTCATCCA TAGTTGCCTG ACTCCCCGTC GTGTAGATAA CTACGATACG
     1801 GGAGGGCTTA CCATCTGGCC CCAGTGCTGC AATGATACCG CGAGAACCAC GCTCACCGGC
     1861 TCCAGATTTA TCAGCAATAA ACCAGCCAGC CGGAAGGGCC GAGCGCAGAA GTGGTCCTGC
     1921 AACTTTATCC GCCTCCATCC AGTCTATTAA TTGTTGCCGG GAAGCTAGAG TAAGTAGTTC
     1981 GCCAGTTAAT AGTTTGCGCA ACGTTGTTGC CATTGCTACA GGCATCGTGG TGTCACGCTC
     2041 GTCGTTTGGT ATGGCTTCAT TCAGCTCCGG TTCCCAACGA TCAAGGCGAG TTACATGATC
     2101 CCCCATGTTG TGCAAAAAAG CGGTTAGCTC CTTCGGTCCT CCGATCGTTG TCAGAAGTAA
     2161 GTTGGCCGCA GTGTTATCAC TCATGGTTAT GGCAGCACTG CATAATTCTC TTACTGTCAT
     2221 GCCATCCGTA AGATGCTTTT CTGTGACTGG TGAGTACTCA ACCAAGTCAT TCTGAGAATA
     2281 GTGTATGCGG CGACCGAGTT GCTCTTGCCC GGCGTCAATA CGGGATAATA CCGCGCCACA
     2341 TAGCAGAACT TTAAAAGTGC TCATCATTGG AAAACGTTCT TCGGGGCGAA AACTCTCAAG
     2401 GATCTTACCG CTGTTGAGAT CCAGTTCGAT GTAACCCACT CGTGCACCCA ACTGATCTTC
     2461 AGCATCTTTT ACTTTCACCA GCGTTTCTGG GTGAGCAAAA ACAGGAAGGC AAAATGCCGC
     2521 AAAAAAGGGA ATAAGGGCGA CACGGAAATG TTGAATACTC ATACTCTTCC TTTTTCAATA
     2581 TTATTGAAGC ATTTATCAGG GTTATTGTCT CATGAGCGGA TACATATTTG AATGTATTTA
     2641 GAAAAATAAA CAAATAGGGG TTCCGCGCAC CTGCACCAGT CAGTAAAACG ACGGCCAGTA
     2701 GTCAAAAGCC TCCGACCGGA GGCTTTTGAC TTGGTTCAGG TGGAGTGGGA GAACGATGAT
     2761 GCTCACTCTC GGGTCTCA
//