LOCUS       BBF10K_003401           2547 bp    DNA     circular     29-Apr-2025
ACCESSION   BBF10K_003401
VERSION     BBF10K_003401.1  
FEATURES             Location/Qualifiers
     CDS             5..427
                     /label=T1T2_CipA
     CDS             complement(1470..2329)
                     /label=AmpR
     promoter        complement(2331..2434)
                     /label=AmpR promoter
     primer_bind     2453..2469
                     /label=M13 fwd
     primer_bind     complement(608..623)
                     /label=M13 rev
     rep_origin      complement(691..1278)
                     /label=ColE1
     primer_bind     525..543
                     /label=SLiCE-for
     primer_bind     complement(2497..2514)
                     /label=SLiCE-rev
     misc_feature    1282..1471
                     /label=spacer
     CDS             642..692
                     /label=spacer
     terminator      558..605
                     /label=T7 terminator
     terminator      2470..2501
                     /label=tonB terminator
     misc_feature    520..2520
                     /label=pOpen_v3 backbone
     misc_feature    2541..2546
                     /label=BsaI_site
     misc_feature    complement(433..438)
                     /label=BsaI_site
     misc_feature    1..4
                     /label=CCAT
     misc_feature    428..431
                     /label=GTCA
ORIGIN
        1 CCATGAATAG CATTAATCCG CAGTTTAAAG TGACCAATAC CGGCAGCAGC GCGATTGATC
       61 TGAGCAAACT GACCCTGCGC TATTATTATA CCGTGGATGG CCAGAAAGAT CAGACCTTTT
      121 GGTGCGATCA TGCGGCGATT ATTGGCAGCA ATGGCAGCTA TAATGGCATT ACCAGCAATG
      181 TGAAAGGCAC CTTTGTGAAA ATGAGCAGCA GCACCAATAA TGCGGATACC TATCTGGAAA
      241 TTAGCTTTAC CGGCGGCACC CTGGAACCGG GCGCGCATGT GCAGATTCAG GGCCGCTTTG
      301 CGAAAAATGA TTGGAGCAAT TATACCCAGA GCAATGATTA TAGCTTTAAA AGCGCGAGCC
      361 AGTTTGTGGA ATGGGATCAG GTGACCGCGT ATCTGAATGG CGTGCTGGTG TGGGGCAAAG
      421 AACCGGGGTC ACGAGACCAC CCCACTGTTA GATTGAGCTT ATAAAACTCG ATAACCGAAA
      481 TAGGGATAAC AGGGTAATGA TTCCGTGAAG CGCATCCCGC GCTGAGGTGT CAATCGTCGG
      541 AGCCGCTGAG CAATAACTAG CATAACCCCT TGGGGCCTCT AAACGGGTCT TGAGGGGTTT
      601 TTTGCATGGT CATAGCTGTT TCCTGAGAGC TTGGCAGGTG ATGACACACA TTAACAAATT
      661 TCGTGAGGAG TCTCCAGAAG AATGCCATTA ATTTCCATAG GCTCCGCCCC CCTGACGAGC
      721 ATCACAAAAA TCGACGCTCA AGTCAGAGGT GGCGAAACCC GACAGGACTA TAAAGATACC
      781 AGGCGTTTCC CCCTGGAAGC TCCCTCGTGC GCTCTCCTGT TCCGACCCTG CCGCTTACCG
      841 GATACCTGTC CGCCTTTCTC CCTTCGGGAA GCGTGGCGCT TTCTCATAGC TCACGCTGTA
      901 GGTATCTCAG TTCGGTGTAG GTCGTTCGCT CCAAGCTGGG CTGTGTGCAC GAACCCCCCG
      961 TTCAGCCCGA CCGCTGCGCC TTATCCGGTA ACTATCGTCT TGAGTCCAAC CCGGTAAGAC
     1021 ACGACTTATC GCCACTGGCA GCAGCCACTG GTAACAGGAT TAGCAGAGCG AGGTATGTAG
     1081 GCGGTGCTAC AGAGTTCTTG AAGTGGTGGC CTAACTACGG CTACACTAGA AGAACAGTAT
     1141 TTGGTATCTG CGCTCTGCTG AAGCCAGTTA CCTTCGGAAA AAGAGTTGGT AGCTCTTGAT
     1201 CCGGCAAACA AACCACCGCT GGTAGCGGTG GTTTTTTTGT TTGCAAGCAG CAGATTACGC
     1261 GCAGAAAAAA AGGATCTCAA GAAGGCCTAC TATTAGCAAC AACGATCCTT TGATCTTTTC
     1321 TACGGGGTCT GACGCTCAGT GGAACGAAAA CTCACGTTAA GGGATTTTGG TCATGAGATT
     1381 ATCAAAAAGG ATCTTCACCT AGATCCTTTT AAATTAAAAA TGAAGTTTTA AATCAATCTA
     1441 AAGTATATAT GAGTAAACTT GGTCTGACAG TTACCAATGC TTAATCAGTG AGGCACCTAT
     1501 CTCAGCGATC TGTCTATTTC GTTCATCCAT AGTTGCCTGA CTCCCCGTCG TGTAGATAAC
     1561 TACGATACGG GAGGGCTTAC CATCTGGCCC CAGTGCTGCA ATGATACCGC GAGAACCACG
     1621 CTCACCGGCT CCAGATTTAT CAGCAATAAA CCAGCCAGCC GGAAGGGCCG AGCGCAGAAG
     1681 TGGTCCTGCA ACTTTATCCG CCTCCATCCA GTCTATTAAT TGTTGCCGGG AAGCTAGAGT
     1741 AAGTAGTTCG CCAGTTAATA GTTTGCGCAA CGTTGTTGCC ATTGCTACAG GCATCGTGGT
     1801 GTCACGCTCG TCGTTTGGTA TGGCTTCATT CAGCTCCGGT TCCCAACGAT CAAGGCGAGT
     1861 TACATGATCC CCCATGTTGT GCAAAAAAGC GGTTAGCTCC TTCGGTCCTC CGATCGTTGT
     1921 CAGAAGTAAG TTGGCCGCAG TGTTATCACT CATGGTTATG GCAGCACTGC ATAATTCTCT
     1981 TACTGTCATG CCATCCGTAA GATGCTTTTC TGTGACTGGT GAGTACTCAA CCAAGTCATT
     2041 CTGAGAATAG TGTATGCGGC GACCGAGTTG CTCTTGCCCG GCGTCAATAC GGGATAATAC
     2101 CGCGCCACAT AGCAGAACTT TAAAAGTGCT CATCATTGGA AAACGTTCTT CGGGGCGAAA
     2161 ACTCTCAAGG ATCTTACCGC TGTTGAGATC CAGTTCGATG TAACCCACTC GTGCACCCAA
     2221 CTGATCTTCA GCATCTTTTA CTTTCACCAG CGTTTCTGGG TGAGCAAAAA CAGGAAGGCA
     2281 AAATGCCGCA AAAAAGGGAA TAAGGGCGAC ACGGAAATGT TGAATACTCA TACTCTTCCT
     2341 TTTTCAATAT TATTGAAGCA TTTATCAGGG TTATTGTCTC ATGAGCGGAT ACATATTTGA
     2401 ATGTATTTAG AAAAATAAAC AAATAGGGGT TCCGCGCACC TGCACCAGTC AGTAAAACGA
     2461 CGGCCAGTAG TCAAAAGCCT CCGACCGGAG GCTTTTGACT TGGTTCAGGT GGAGTGGGAG
     2521 AACGATGATG CTCACTCTCG GGTCTCA
//