LOCUS       BBF10K_003402           2472 bp    DNA     circular     29-Apr-2025
ACCESSION   BBF10K_003402
VERSION     BBF10K_003402.1  
FEATURES             Location/Qualifiers
     CDS             5..52
                     /label=T1T2_S-Tag
     CDS             complement(1245..2104)
                     /label=AmpR
     promoter        complement(2106..2209)
                     /label=AmpR promoter
     primer_bind     2228..2244
                     /label=M13 fwd
     primer_bind     complement(383..398)
                     /label=M13 rev
     rep_origin      complement(466..1053)
                     /label=ColE1
     primer_bind     300..318
                     /label=SLiCE-for
     primer_bind     complement(2272..2289)
                     /label=SLiCE-rev
     misc_feature    1057..1246
                     /label=spacer
     CDS             417..467
                     /label=spacer
     terminator      333..380
                     /label=T7 terminator
     terminator      2245..2276
                     /label=tonB terminator
     misc_feature    295..2295
                     /label=pOpen_v3 backbone
     misc_feature    2466..2471
                     /label=BsaI_site
     misc_feature    complement(58..63)
                     /label=BsaI_site
     misc_feature    1..4
                     /label=CCAT
     misc_feature    53..56
                     /label=GTCA
ORIGIN
        1 CCATGAAAGA AACCGCTGCT GCTAAATTCG AACGCCAGCA CATGGACAGC GGGTCACGAG
       61 ACCGAAAGAG ATGTCAGTTT GCTGGTTCAC GTAAAAGTTC CTCACACTAC CTCTAAATAA
      121 GAGAACGGTC GTAACATTAT CCGTGATTTT CTCACTACTA TTAGTACTCA CGACACGATT
      181 CTGCCACAGC CTTGTTTCGC CAGAATGCCA GTCACTTCAT TGGTCTGGTG TCCTATACCA
      241 GGACGCAGCG AAGAGTAGGG ATAACAGGGT AATCCAGACG GACAACTCGC TCTGCGCTGA
      301 GGTGTCAATC GTCGGAGCCG CTGAGCAATA ACTAGCATAA CCCCTTGGGG CCTCTAAACG
      361 GGTCTTGAGG GGTTTTTTGC ATGGTCATAG CTGTTTCCTG AGAGCTTGGC AGGTGATGAC
      421 ACACATTAAC AAATTTCGTG AGGAGTCTCC AGAAGAATGC CATTAATTTC CATAGGCTCC
      481 GCCCCCCTGA CGAGCATCAC AAAAATCGAC GCTCAAGTCA GAGGTGGCGA AACCCGACAG
      541 GACTATAAAG ATACCAGGCG TTTCCCCCTG GAAGCTCCCT CGTGCGCTCT CCTGTTCCGA
      601 CCCTGCCGCT TACCGGATAC CTGTCCGCCT TTCTCCCTTC GGGAAGCGTG GCGCTTTCTC
      661 ATAGCTCACG CTGTAGGTAT CTCAGTTCGG TGTAGGTCGT TCGCTCCAAG CTGGGCTGTG
      721 TGCACGAACC CCCCGTTCAG CCCGACCGCT GCGCCTTATC CGGTAACTAT CGTCTTGAGT
      781 CCAACCCGGT AAGACACGAC TTATCGCCAC TGGCAGCAGC CACTGGTAAC AGGATTAGCA
      841 GAGCGAGGTA TGTAGGCGGT GCTACAGAGT TCTTGAAGTG GTGGCCTAAC TACGGCTACA
      901 CTAGAAGAAC AGTATTTGGT ATCTGCGCTC TGCTGAAGCC AGTTACCTTC GGAAAAAGAG
      961 TTGGTAGCTC TTGATCCGGC AAACAAACCA CCGCTGGTAG CGGTGGTTTT TTTGTTTGCA
     1021 AGCAGCAGAT TACGCGCAGA AAAAAAGGAT CTCAAGAAGG CCTACTATTA GCAACAACGA
     1081 TCCTTTGATC TTTTCTACGG GGTCTGACGC TCAGTGGAAC GAAAACTCAC GTTAAGGGAT
     1141 TTTGGTCATG AGATTATCAA AAAGGATCTT CACCTAGATC CTTTTAAATT AAAAATGAAG
     1201 TTTTAAATCA ATCTAAAGTA TATATGAGTA AACTTGGTCT GACAGTTACC AATGCTTAAT
     1261 CAGTGAGGCA CCTATCTCAG CGATCTGTCT ATTTCGTTCA TCCATAGTTG CCTGACTCCC
     1321 CGTCGTGTAG ATAACTACGA TACGGGAGGG CTTACCATCT GGCCCCAGTG CTGCAATGAT
     1381 ACCGCGAGAA CCACGCTCAC CGGCTCCAGA TTTATCAGCA ATAAACCAGC CAGCCGGAAG
     1441 GGCCGAGCGC AGAAGTGGTC CTGCAACTTT ATCCGCCTCC ATCCAGTCTA TTAATTGTTG
     1501 CCGGGAAGCT AGAGTAAGTA GTTCGCCAGT TAATAGTTTG CGCAACGTTG TTGCCATTGC
     1561 TACAGGCATC GTGGTGTCAC GCTCGTCGTT TGGTATGGCT TCATTCAGCT CCGGTTCCCA
     1621 ACGATCAAGG CGAGTTACAT GATCCCCCAT GTTGTGCAAA AAAGCGGTTA GCTCCTTCGG
     1681 TCCTCCGATC GTTGTCAGAA GTAAGTTGGC CGCAGTGTTA TCACTCATGG TTATGGCAGC
     1741 ACTGCATAAT TCTCTTACTG TCATGCCATC CGTAAGATGC TTTTCTGTGA CTGGTGAGTA
     1801 CTCAACCAAG TCATTCTGAG AATAGTGTAT GCGGCGACCG AGTTGCTCTT GCCCGGCGTC
     1861 AATACGGGAT AATACCGCGC CACATAGCAG AACTTTAAAA GTGCTCATCA TTGGAAAACG
     1921 TTCTTCGGGG CGAAAACTCT CAAGGATCTT ACCGCTGTTG AGATCCAGTT CGATGTAACC
     1981 CACTCGTGCA CCCAACTGAT CTTCAGCATC TTTTACTTTC ACCAGCGTTT CTGGGTGAGC
     2041 AAAAACAGGA AGGCAAAATG CCGCAAAAAA GGGAATAAGG GCGACACGGA AATGTTGAAT
     2101 ACTCATACTC TTCCTTTTTC AATATTATTG AAGCATTTAT CAGGGTTATT GTCTCATGAG
     2161 CGGATACATA TTTGAATGTA TTTAGAAAAA TAAACAAATA GGGGTTCCGC GCACCTGCAC
     2221 CAGTCAGTAA AACGACGGCC AGTAGTCAAA AGCCTCCGAC CGGAGGCTTT TGACTTGGTT
     2281 CAGGTGGAGT GGGAGAACGA TGATGCTCAC TCTCGAGCAT AAAGGAGAGC TCAAAGCAGG
     2341 TCAACTCGCA TAGTGAGAGT TACATGATCG TTCGGCTCTA CCGACACGAA CCTCAGTTAG
     2401 CGTACATCCT ACCAGAGGTC TGTGGCCCCG TGATGAGAAG TGTGGATTTC GTATTTGCAG
     2461 CTCGTGGTCT CA
//