LOCUS       BBF10K_003403           2550 bp    DNA     circular     17-Jun-2025
ACCESSION   BBF10K_003403
VERSION     BBF10K_003403.1  
FEATURES             Location/Qualifiers
     CDS             5..172
                     /label=T1T2_GB1
     CDS             complement(1343..2202)
                     /label=AmpR
     promoter        complement(2204..2307)
                     /label=AmpR promoter
     primer_bind     2326..2342
                     /label=M13 fwd
     primer_bind     complement(481..496)
                     /label=M13 rev
     rep_origin      complement(564..1151)
                     /label=ColE1
     primer_bind     398..416
                     /label=SLiCE-for
     primer_bind     complement(2370..2387)
                     /label=SLiCE-rev
     misc_feature    1155..1344
                     /label=spacer
     CDS             515..565
                     /label=spacer
     terminator      431..478
                     /label=T7 terminator
     terminator      2343..2374
                     /label=tonB terminator
     misc_feature    393..2393
                     /label=pOpen_v3 backbone
     misc_feature    2544..2549
                     /label=BsaI_site
     misc_feature    complement(178..183)
                     /label=BsaI_site
     misc_feature    1..4
                     /label=CCAT
     misc_feature    173..176
                     /label=GTCA
ORIGIN
        1 CCATGCAGTA CAAACTGATC CTGAACGGTA AAACCCTGAA AGGTGAAACC ACCACCGAAG
       61 CTGTTGACGC TGCTACCGCG GAAAAAGTTT TCAAACAGTA CGCTAACGAC AACGGTGTTG
      121 ACGGTGAATG GACCTACGAC GACGCTACCA AAACCTTCAC CGTTACCGAA GGGTCACGAG
      181 ACCGGCCCAT ATCTGGAAAT AGACGCCAAG TTCAATCCGT ATTCCGACGT ACGATGGAAC
      241 AGTGTGGATA GACGTGCTTC ATTTATACCC TACGCAGGCT GGACGAGGTC CGCAAGGCGC
      301 AGCAGTGCAC AACGCTGATG GTAATTAGCC CTTCGGTCGG TGTACCGACC AGGTAGGGAT
      361 AACAGGGTAA TCACCCTTGG GAGGAATACT CGCGCTGAGG TGTCAATCGT CGGAGCCGCT
      421 GAGCAATAAC TAGCATAACC CCTTGGGGCC TCTAAACGGG TCTTGAGGGG TTTTTTGCAT
      481 GGTCATAGCT GTTTCCTGAG AGCTTGGCAG GTGATGACAC ACATTAACAA ATTTCGTGAG
      541 GAGTCTCCAG AAGAATGCCA TTAATTTCCA TAGGCTCCGC CCCCCTGACG AGCATCACAA
      601 AAATCGACGC TCAAGTCAGA GGTGGCGAAA CCCGACAGGA CTATAAAGAT ACCAGGCGTT
      661 TCCCCCTGGA AGCTCCCTCG TGCGCTCTCC TGTTCCGACC CTGCCGCTTA CCGGATACCT
      721 GTCCGCCTTT CTCCCTTCGG GAAGCGTGGC GCTTTCTCAT AGCTCACGCT GTAGGTATCT
      781 CAGTTCGGTG TAGGTCGTTC GCTCCAAGCT GGGCTGTGTG CACGAACCCC CCGTTCAGCC
      841 CGACCGCTGC GCCTTATCCG GTAACTATCG TCTTGAGTCC AACCCGGTAA GACACGACTT
      901 ATCGCCACTG GCAGCAGCCA CTGGTAACAG GATTAGCAGA GCGAGGTATG TAGGCGGTGC
      961 TACAGAGTTC TTGAAGTGGT GGCCTAACTA CGGCTACACT AGAAGAACAG TATTTGGTAT
     1021 CTGCGCTCTG CTGAAGCCAG TTACCTTCGG AAAAAGAGTT GGTAGCTCTT GATCCGGCAA
     1081 ACAAACCACC GCTGGTAGCG GTGGTTTTTT TGTTTGCAAG CAGCAGATTA CGCGCAGAAA
     1141 AAAAGGATCT CAAGAAGGCC TACTATTAGC AACAACGATC CTTTGATCTT TTCTACGGGG
     1201 TCTGACGCTC AGTGGAACGA AAACTCACGT TAAGGGATTT TGGTCATGAG ATTATCAAAA
     1261 AGGATCTTCA CCTAGATCCT TTTAAATTAA AAATGAAGTT TTAAATCAAT CTAAAGTATA
     1321 TATGAGTAAA CTTGGTCTGA CAGTTACCAA TGCTTAATCA GTGAGGCACC TATCTCAGCG
     1381 ATCTGTCTAT TTCGTTCATC CATAGTTGCC TGACTCCCCG TCGTGTAGAT AACTACGATA
     1441 CGGGAGGGCT TACCATCTGG CCCCAGTGCT GCAATGATAC CGCGAGAACC ACGCTCACCG
     1501 GCTCCAGATT TATCAGCAAT AAACCAGCCA GCCGGAAGGG CCGAGCGCAG AAGTGGTCCT
     1561 GCAACTTTAT CCGCCTCCAT CCAGTCTATT AATTGTTGCC GGGAAGCTAG AGTAAGTAGT
     1621 TCGCCAGTTA ATAGTTTGCG CAACGTTGTT GCCATTGCTA CAGGCATCGT GGTGTCACGC
     1681 TCGTCGTTTG GTATGGCTTC ATTCAGCTCC GGTTCCCAAC GATCAAGGCG AGTTACATGA
     1741 TCCCCCATGT TGTGCAAAAA AGCGGTTAGC TCCTTCGGTC CTCCGATCGT TGTCAGAAGT
     1801 AAGTTGGCCG CAGTGTTATC ACTCATGGTT ATGGCAGCAC TGCATAATTC TCTTACTGTC
     1861 ATGCCATCCG TAAGATGCTT TTCTGTGACT GGTGAGTACT CAACCAAGTC ATTCTGAGAA
     1921 TAGTGTATGC GGCGACCGAG TTGCTCTTGC CCGGCGTCAA TACGGGATAA TACCGCGCCA
     1981 CATAGCAGAA CTTTAAAAGT GCTCATCATT GGAAAACGTT CTTCGGGGCG AAAACTCTCA
     2041 AGGATCTTAC CGCTGTTGAG ATCCAGTTCG ATGTAACCCA CTCGTGCACC CAACTGATCT
     2101 TCAGCATCTT TTACTTTCAC CAGCGTTTCT GGGTGAGCAA AAACAGGAAG GCAAAATGCC
     2161 GCAAAAAAGG GAATAAGGGC GACACGGAAA TGTTGAATAC TCATACTCTT CCTTTTTCAA
     2221 TATTATTGAA GCATTTATCA GGGTTATTGT CTCATGAGCG GATACATATT TGAATGTATT
     2281 TAGAAAAATA AACAAATAGG GGTTCCGCGC ACCTGCACCA GTCAGTAAAA CGACGGCCAG
     2341 TAGTCAAAAG CCTCCGACCG GAGGCTTTTG ACTTGGTTCA GGTGGAGTGG GAGAACGATG
     2401 ATGCTCACTC TCGAGCAATT GACAATTAAC CACCGTGTAT TCATTATAGT ATCAGGGACT
     2461 TTAAGTCGAG TCAATAGAGC TCGCAATACA GAGTTTACCG CATCTTGTCG TAACTGACAA
     2521 ACTGTGATCC ACCACAAGTC AAGGGTCTCA
//