LOCUS       BBF10K_003405           2418 bp    DNA     circular     18-Jun-2025
ACCESSION   BBF10K_003405
VERSION     BBF10K_003405.1  
FEATURES             Location/Qualifiers
     CDS             5..38
                     /label=T1T3_GS-flexible
     CDS             complement(1211..2070)
                     /label=AmpR
     promoter        complement(2072..2175)
                     /label=AmpR promoter
     primer_bind     2194..2210
                     /label=M13 fwd
     primer_bind     complement(349..364)
                     /label=M13 rev
     rep_origin      complement(432..1019)
                     /label=ColE1
     primer_bind     266..284
                     /label=SLiCE-for
     primer_bind     complement(2238..2255)
                     /label=SLiCE-rev
     misc_feature    1023..1212
                     /label=spacer
     CDS             383..433
                     /label=spacer
     terminator      299..346
                     /label=T7 terminator
     terminator      2211..2242
                     /label=tonB terminator
     misc_feature    261..2261
                     /label=pOpen_v3 backbone
     misc_feature    2412..2417
                     /label=BsaI_site
     misc_feature    complement(44..49)
                     /label=BsaI_site
     misc_feature    1..4
                     /label=CCAT
     misc_feature    39..42
                     /label=TCCA
ORIGIN
        1 CCATGGGAGG TGGCTCTGGC GGTGGATCAG GTGGAGGCTC CACGAGACCG CAGATAGATT
       61 GCCAAGCATA GAGCTTACGA GCCAGCGAAA ACAGTAAGGC CTTTTTAAGT ATGGGGAGTA
      121 AGTGATCGAA CGCTTCAGAT ATGACTATAT ACTTAGGTTA GATCTCGTCC CGTGAATTTA
      181 GCTGGGCACT ATGCTTTACT TCGCGCGGGT CGAGCGCCCC ATAGGGATAA CAGGGTAATC
      241 GGCTCTCCCA CTAAACCTGA CGCTGAGGTG TCAATCGTCG GAGCCGCTGA GCAATAACTA
      301 GCATAACCCC TTGGGGCCTC TAAACGGGTC TTGAGGGGTT TTTTGCATGG TCATAGCTGT
      361 TTCCTGAGAG CTTGGCAGGT GATGACACAC ATTAACAAAT TTCGTGAGGA GTCTCCAGAA
      421 GAATGCCATT AATTTCCATA GGCTCCGCCC CCCTGACGAG CATCACAAAA ATCGACGCTC
      481 AAGTCAGAGG TGGCGAAACC CGACAGGACT ATAAAGATAC CAGGCGTTTC CCCCTGGAAG
      541 CTCCCTCGTG CGCTCTCCTG TTCCGACCCT GCCGCTTACC GGATACCTGT CCGCCTTTCT
      601 CCCTTCGGGA AGCGTGGCGC TTTCTCATAG CTCACGCTGT AGGTATCTCA GTTCGGTGTA
      661 GGTCGTTCGC TCCAAGCTGG GCTGTGTGCA CGAACCCCCC GTTCAGCCCG ACCGCTGCGC
      721 CTTATCCGGT AACTATCGTC TTGAGTCCAA CCCGGTAAGA CACGACTTAT CGCCACTGGC
      781 AGCAGCCACT GGTAACAGGA TTAGCAGAGC GAGGTATGTA GGCGGTGCTA CAGAGTTCTT
      841 GAAGTGGTGG CCTAACTACG GCTACACTAG AAGAACAGTA TTTGGTATCT GCGCTCTGCT
      901 GAAGCCAGTT ACCTTCGGAA AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC AAACCACCGC
      961 TGGTAGCGGT GGTTTTTTTG TTTGCAAGCA GCAGATTACG CGCAGAAAAA AAGGATCTCA
     1021 AGAAGGCCTA CTATTAGCAA CAACGATCCT TTGATCTTTT CTACGGGGTC TGACGCTCAG
     1081 TGGAACGAAA ACTCACGTTA AGGGATTTTG GTCATGAGAT TATCAAAAAG GATCTTCACC
     1141 TAGATCCTTT TAAATTAAAA ATGAAGTTTT AAATCAATCT AAAGTATATA TGAGTAAACT
     1201 TGGTCTGACA GTTACCAATG CTTAATCAGT GAGGCACCTA TCTCAGCGAT CTGTCTATTT
     1261 CGTTCATCCA TAGTTGCCTG ACTCCCCGTC GTGTAGATAA CTACGATACG GGAGGGCTTA
     1321 CCATCTGGCC CCAGTGCTGC AATGATACCG CGAGAACCAC GCTCACCGGC TCCAGATTTA
     1381 TCAGCAATAA ACCAGCCAGC CGGAAGGGCC GAGCGCAGAA GTGGTCCTGC AACTTTATCC
     1441 GCCTCCATCC AGTCTATTAA TTGTTGCCGG GAAGCTAGAG TAAGTAGTTC GCCAGTTAAT
     1501 AGTTTGCGCA ACGTTGTTGC CATTGCTACA GGCATCGTGG TGTCACGCTC GTCGTTTGGT
     1561 ATGGCTTCAT TCAGCTCCGG TTCCCAACGA TCAAGGCGAG TTACATGATC CCCCATGTTG
     1621 TGCAAAAAAG CGGTTAGCTC CTTCGGTCCT CCGATCGTTG TCAGAAGTAA GTTGGCCGCA
     1681 GTGTTATCAC TCATGGTTAT GGCAGCACTG CATAATTCTC TTACTGTCAT GCCATCCGTA
     1741 AGATGCTTTT CTGTGACTGG TGAGTACTCA ACCAAGTCAT TCTGAGAATA GTGTATGCGG
     1801 CGACCGAGTT GCTCTTGCCC GGCGTCAATA CGGGATAATA CCGCGCCACA TAGCAGAACT
     1861 TTAAAAGTGC TCATCATTGG AAAACGTTCT TCGGGGCGAA AACTCTCAAG GATCTTACCG
     1921 CTGTTGAGAT CCAGTTCGAT GTAACCCACT CGTGCACCCA ACTGATCTTC AGCATCTTTT
     1981 ACTTTCACCA GCGTTTCTGG GTGAGCAAAA ACAGGAAGGC AAAATGCCGC AAAAAAGGGA
     2041 ATAAGGGCGA CACGGAAATG TTGAATACTC ATACTCTTCC TTTTTCAATA TTATTGAAGC
     2101 ATTTATCAGG GTTATTGTCT CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA
     2161 CAAATAGGGG TTCCGCGCAC CTGCACCAGT CAGTAAAACG ACGGCCAGTA GTCAAAAGCC
     2221 TCCGACCGGA GGCTTTTGAC TTGGTTCAGG TGGAGTGGGA GAACGATGAT GCTCACTCTC
     2281 GTAATCCTCA TCAATTATAA AATATGAGGT AAGCCAAAAA AGCACGTGGT GGCGCTCACC
     2341 GACTGTTCCC AAACTGTAAC TCATTGTTCT GTCAAGGCCT GACTTACTTC CCGGCCCTTT
     2401 CCATGTGCGG AGGTCTCA
//