LOCUS BBF10K_003406 2417 bp DNA circular 29-Apr-2025 ACCESSION BBF10K_003406 VERSION BBF10K_003406.1 FEATURES Location/Qualifiers CDS 5..40 /label=T1C_GS-flexible CDS complement(1212..2071) /label=AmpR promoter complement(2073..2176) /label=AmpR promoter primer_bind 2195..2211 /label=M13 fwd primer_bind complement(350..365) /label=M13 rev rep_origin complement(433..1020) /label=ColE1 primer_bind 267..285 /label=SLiCE-for primer_bind complement(2239..2256) /label=SLiCE-rev misc_feature 1024..1213 /label=spacer CDS 384..434 /label=spacer terminator 300..347 /label=T7 terminator terminator 2212..2243 /label=tonB terminator misc_feature 262..2262 /label=pOpen_v3 backbone misc_feature 2411..2416 /label=BsaI_site misc_feature complement(46..51) /label=BsaI_site misc_feature 1..4 /label=CCAT misc_feature 41..44 /label=AATG ORIGIN 1 CCATGGGAGG TGGCTCTGGC GGTGGATCAG GTGGAGGCTC AATGCGAGAC CACTCCGGTC 61 CGTTCTGAGT CGACCAAGGA CACAATCGAG CTCCGATCTG TATTGTCGAG AAACTTGTAT 121 CCAACCCCCG CAGCTTGCCA GCTCTTGGGT ATCATGGAGC CTATGGTTGA ACGTGTCCGA 181 AGCAGCGATT CGGATACTAC TGCTATGGAA ACCGCTTCCC CGTAGGGATA ACAGGGTAAT 241 TATTTACTCG TTGAACGGCT GCGCTGAGGT GTCAATCGTC GGAGCCGCTG AGCAATAACT 301 AGCATAACCC CTTGGGGCCT CTAAACGGGT CTTGAGGGGT TTTTTGCATG GTCATAGCTG 361 TTTCCTGAGA GCTTGGCAGG TGATGACACA CATTAACAAA TTTCGTGAGG AGTCTCCAGA 421 AGAATGCCAT TAATTTCCAT AGGCTCCGCC CCCCTGACGA GCATCACAAA AATCGACGCT 481 CAAGTCAGAG GTGGCGAAAC CCGACAGGAC TATAAAGATA CCAGGCGTTT CCCCCTGGAA 541 GCTCCCTCGT GCGCTCTCCT GTTCCGACCC TGCCGCTTAC CGGATACCTG TCCGCCTTTC 601 TCCCTTCGGG AAGCGTGGCG CTTTCTCATA GCTCACGCTG TAGGTATCTC AGTTCGGTGT 661 AGGTCGTTCG CTCCAAGCTG GGCTGTGTGC ACGAACCCCC CGTTCAGCCC GACCGCTGCG 721 CCTTATCCGG TAACTATCGT CTTGAGTCCA ACCCGGTAAG ACACGACTTA TCGCCACTGG 781 CAGCAGCCAC TGGTAACAGG ATTAGCAGAG CGAGGTATGT AGGCGGTGCT ACAGAGTTCT 841 TGAAGTGGTG GCCTAACTAC GGCTACACTA GAAGAACAGT ATTTGGTATC TGCGCTCTGC 901 TGAAGCCAGT TACCTTCGGA AAAAGAGTTG GTAGCTCTTG ATCCGGCAAA CAAACCACCG 961 CTGGTAGCGG TGGTTTTTTT GTTTGCAAGC AGCAGATTAC GCGCAGAAAA AAAGGATCTC 1021 AAGAAGGCCT ACTATTAGCA ACAACGATCC TTTGATCTTT TCTACGGGGT CTGACGCTCA 1081 GTGGAACGAA AACTCACGTT AAGGGATTTT GGTCATGAGA TTATCAAAAA GGATCTTCAC 1141 CTAGATCCTT TTAAATTAAA AATGAAGTTT TAAATCAATC TAAAGTATAT ATGAGTAAAC 1201 TTGGTCTGAC AGTTACCAAT GCTTAATCAG TGAGGCACCT ATCTCAGCGA TCTGTCTATT 1261 TCGTTCATCC ATAGTTGCCT GACTCCCCGT CGTGTAGATA ACTACGATAC GGGAGGGCTT 1321 ACCATCTGGC CCCAGTGCTG CAATGATACC GCGAGAACCA CGCTCACCGG CTCCAGATTT 1381 ATCAGCAATA AACCAGCCAG CCGGAAGGGC CGAGCGCAGA AGTGGTCCTG CAACTTTATC 1441 CGCCTCCATC CAGTCTATTA ATTGTTGCCG GGAAGCTAGA GTAAGTAGTT CGCCAGTTAA 1501 TAGTTTGCGC AACGTTGTTG CCATTGCTAC AGGCATCGTG GTGTCACGCT CGTCGTTTGG 1561 TATGGCTTCA TTCAGCTCCG GTTCCCAACG ATCAAGGCGA GTTACATGAT CCCCCATGTT 1621 GTGCAAAAAA GCGGTTAGCT CCTTCGGTCC TCCGATCGTT GTCAGAAGTA AGTTGGCCGC 1681 AGTGTTATCA CTCATGGTTA TGGCAGCACT GCATAATTCT CTTACTGTCA TGCCATCCGT 1741 AAGATGCTTT TCTGTGACTG GTGAGTACTC AACCAAGTCA TTCTGAGAAT AGTGTATGCG 1801 GCGACCGAGT TGCTCTTGCC CGGCGTCAAT ACGGGATAAT ACCGCGCCAC ATAGCAGAAC 1861 TTTAAAAGTG CTCATCATTG GAAAACGTTC TTCGGGGCGA AAACTCTCAA GGATCTTACC 1921 GCTGTTGAGA TCCAGTTCGA TGTAACCCAC TCGTGCACCC AACTGATCTT CAGCATCTTT 1981 TACTTTCACC AGCGTTTCTG GGTGAGCAAA AACAGGAAGG CAAAATGCCG CAAAAAAGGG 2041 AATAAGGGCG ACACGGAAAT GTTGAATACT CATACTCTTC CTTTTTCAAT ATTATTGAAG 2101 CATTTATCAG GGTTATTGTC TCATGAGCGG ATACATATTT GAATGTATTT AGAAAAATAA 2161 ACAAATAGGG GTTCCGCGCA CCTGCACCAG TCAGTAAAAC GACGGCCAGT AGTCAAAAGC 2221 CTCCGACCGG AGGCTTTTGA CTTGGTTCAG GTGGAGTGGG AGAACGATGA TGCTCACTCT 2281 CGTAACAAAC TTCGACATGA TAAAGTTCCC CCCTCGCGAC TACCAGAGAA AAGACTACTG 2341 AATTGAGCGT TCCCAGCACT TCAGCTAAGG AAGCTACCAA TTTTTAGTTT CTAAGTGTTA 2401 CGTCTGACTC GGTCTCA //