LOCUS       BBF10K_003407           2427 bp    DNA     circular     29-Apr-2025
ACCESSION   BBF10K_003407
VERSION     BBF10K_003407.1  
FEATURES             Location/Qualifiers
     CDS             5..67
                     /label=T1C_His_TEVcut
     CDS             complement(1230..2089)
                     /label=AmpR
     promoter        complement(2091..2194)
                     /label=AmpR promoter
     primer_bind     2213..2229
                     /label=M13 fwd
     primer_bind     complement(368..383)
                     /label=M13 rev
     rep_origin      complement(451..1038)
                     /label=ColE1
     primer_bind     285..303
                     /label=SLiCE-for
     primer_bind     complement(2257..2274)
                     /label=SLiCE-rev
     misc_feature    1042..1231
                     /label=spacer
     CDS             402..452
                     /label=spacer
     terminator      318..365
                     /label=T7 terminator
     terminator      2230..2261
                     /label=tonB terminator
     misc_feature    280..2280
                     /label=pOpen_v3 backbone
     misc_feature    2421..2426
                     /label=BsaI_site
     misc_feature    complement(73..78)
                     /label=BsaI_site
     misc_feature    1..4
                     /label=CCAT
     misc_feature    68..71
                     /label=AATG
ORIGIN
        1 CCATGGGCAG CAGCCATCAC CATCACCATC ACAGCAGCGG CGGAGAAAAT CTTTATTTTC
       61 AAGGTTCAAT GCGAGACCGA TGTTGAAGAC TCAGGTTATA CAAGCACAAG TACTATATAT
      121 ACGTGTTCCG GCTCTTAACC TGGATCGAAA GCACAATCAT GCATCGTACC AGTGTATTCG
      181 TGTCATCTAG GACGGGCGAG TTGCCCTTGC TGAGATACTG GTAACCTAGT TAGCCGTAAT
      241 TAGGGATAAC AGGGTAATGG GGAGGGGCGG TGGTGGCCTC GCTGAGGTGT CAATCGTCGG
      301 AGCCGCTGAG CAATAACTAG CATAACCCCT TGGGGCCTCT AAACGGGTCT TGAGGGGTTT
      361 TTTGCATGGT CATAGCTGTT TCCTGAGAGC TTGGCAGGTG ATGACACACA TTAACAAATT
      421 TCGTGAGGAG TCTCCAGAAG AATGCCATTA ATTTCCATAG GCTCCGCCCC CCTGACGAGC
      481 ATCACAAAAA TCGACGCTCA AGTCAGAGGT GGCGAAACCC GACAGGACTA TAAAGATACC
      541 AGGCGTTTCC CCCTGGAAGC TCCCTCGTGC GCTCTCCTGT TCCGACCCTG CCGCTTACCG
      601 GATACCTGTC CGCCTTTCTC CCTTCGGGAA GCGTGGCGCT TTCTCATAGC TCACGCTGTA
      661 GGTATCTCAG TTCGGTGTAG GTCGTTCGCT CCAAGCTGGG CTGTGTGCAC GAACCCCCCG
      721 TTCAGCCCGA CCGCTGCGCC TTATCCGGTA ACTATCGTCT TGAGTCCAAC CCGGTAAGAC
      781 ACGACTTATC GCCACTGGCA GCAGCCACTG GTAACAGGAT TAGCAGAGCG AGGTATGTAG
      841 GCGGTGCTAC AGAGTTCTTG AAGTGGTGGC CTAACTACGG CTACACTAGA AGAACAGTAT
      901 TTGGTATCTG CGCTCTGCTG AAGCCAGTTA CCTTCGGAAA AAGAGTTGGT AGCTCTTGAT
      961 CCGGCAAACA AACCACCGCT GGTAGCGGTG GTTTTTTTGT TTGCAAGCAG CAGATTACGC
     1021 GCAGAAAAAA AGGATCTCAA GAAGGCCTAC TATTAGCAAC AACGATCCTT TGATCTTTTC
     1081 TACGGGGTCT GACGCTCAGT GGAACGAAAA CTCACGTTAA GGGATTTTGG TCATGAGATT
     1141 ATCAAAAAGG ATCTTCACCT AGATCCTTTT AAATTAAAAA TGAAGTTTTA AATCAATCTA
     1201 AAGTATATAT GAGTAAACTT GGTCTGACAG TTACCAATGC TTAATCAGTG AGGCACCTAT
     1261 CTCAGCGATC TGTCTATTTC GTTCATCCAT AGTTGCCTGA CTCCCCGTCG TGTAGATAAC
     1321 TACGATACGG GAGGGCTTAC CATCTGGCCC CAGTGCTGCA ATGATACCGC GAGAACCACG
     1381 CTCACCGGCT CCAGATTTAT CAGCAATAAA CCAGCCAGCC GGAAGGGCCG AGCGCAGAAG
     1441 TGGTCCTGCA ACTTTATCCG CCTCCATCCA GTCTATTAAT TGTTGCCGGG AAGCTAGAGT
     1501 AAGTAGTTCG CCAGTTAATA GTTTGCGCAA CGTTGTTGCC ATTGCTACAG GCATCGTGGT
     1561 GTCACGCTCG TCGTTTGGTA TGGCTTCATT CAGCTCCGGT TCCCAACGAT CAAGGCGAGT
     1621 TACATGATCC CCCATGTTGT GCAAAAAAGC GGTTAGCTCC TTCGGTCCTC CGATCGTTGT
     1681 CAGAAGTAAG TTGGCCGCAG TGTTATCACT CATGGTTATG GCAGCACTGC ATAATTCTCT
     1741 TACTGTCATG CCATCCGTAA GATGCTTTTC TGTGACTGGT GAGTACTCAA CCAAGTCATT
     1801 CTGAGAATAG TGTATGCGGC GACCGAGTTG CTCTTGCCCG GCGTCAATAC GGGATAATAC
     1861 CGCGCCACAT AGCAGAACTT TAAAAGTGCT CATCATTGGA AAACGTTCTT CGGGGCGAAA
     1921 ACTCTCAAGG ATCTTACCGC TGTTGAGATC CAGTTCGATG TAACCCACTC GTGCACCCAA
     1981 CTGATCTTCA GCATCTTTTA CTTTCACCAG CGTTTCTGGG TGAGCAAAAA CAGGAAGGCA
     2041 AAATGCCGCA AAAAAGGGAA TAAGGGCGAC ACGGAAATGT TGAATACTCA TACTCTTCCT
     2101 TTTTCAATAT TATTGAAGCA TTTATCAGGG TTATTGTCTC ATGAGCGGAT ACATATTTGA
     2161 ATGTATTTAG AAAAATAAAC AAATAGGGGT TCCGCGCACC TGCACCAGTC AGTAAAACGA
     2221 CGGCCAGTAG TCAAAAGCCT CCGACCGGAG GCTTTTGACT TGGTTCAGGT GGAGTGGGAG
     2281 AACGATGATG CTCACTCTCG CATAGGATAT ATAATTCAAT TAAGAATACC TTATACTACT
     2341 ATACACCTAC CCGTCACCGG CCAACAATGT GCAGATGGCG CTACGACTTA CTGGGCCTGA
     2401 TTTCACCGCT TTTAATACCA GGTCTCA
//