LOCUS       BBF10K_003408           2441 bp    DNA     circular     29-Apr-2025
ACCESSION   BBF10K_003408
VERSION     BBF10K_003408.1  
FEATURES             Location/Qualifiers
     misc_feature    5..121
                     /label=BT2_BCD2_His
     CDS             complement(1264..2123)
                     /label=AmpR
     promoter        complement(2125..2228)
                     /label=AmpR promoter
     primer_bind     2247..2263
                     /label=M13 fwd
     primer_bind     complement(402..417)
                     /label=M13 rev
     rep_origin      complement(485..1072)
                     /label=ColE1
     primer_bind     319..337
                     /label=SLiCE-for
     primer_bind     complement(2291..2308)
                     /label=SLiCE-rev
     misc_feature    1076..1265
                     /label=spacer
     CDS             436..486
                     /label=spacer
     terminator      352..399
                     /label=T7 terminator
     terminator      2264..2295
                     /label=tonB terminator
     misc_feature    314..2314
                     /label=pOpen_v3 backbone
     misc_feature    2435..2440
                     /label=BsaI_site
     misc_feature    complement(127..132)
                     /label=BsaI_site
     misc_feature    1..4
                     /label=TACT
     misc_feature    122..125
                     /label=GTCA
ORIGIN
        1 TACTGGGCCC AAGTTCACTT AAAAAGGAGA TCAACAATGA AAGCAATTTT CGTACTGAAA
       61 CATCTTAATC ATGCTAAGGA GGTTTTCTAA TGGGTAGCAG CCATCATCAT CATCATCACG
      121 GGTCACGAGA CCCCAAATTT TAAATCATAT CACGCGACTA GCCTCTGCTT AATTTCTGTG
      181 CTCAAGGGTT TTGGTCCGCC CGAGCGGTGC AGCCGATTAG GACCATCTAA TGAGGACCTT
      241 GACGCCTAAT ACTGGGACAA GTCAACCGGC CGGCTAGGGA TAACAGGGTA ATAAACCCGA
      301 GTTAGCGCGG CGTCGCTGAG GTGTCAATCG TCGGAGCCGC TGAGCAATAA CTAGCATAAC
      361 CCCTTGGGGC CTCTAAACGG GTCTTGAGGG GTTTTTTGCA TGGTCATAGC TGTTTCCTGA
      421 GAGCTTGGCA GGTGATGACA CACATTAACA AATTTCGTGA GGAGTCTCCA GAAGAATGCC
      481 ATTAATTTCC ATAGGCTCCG CCCCCCTGAC GAGCATCACA AAAATCGACG CTCAAGTCAG
      541 AGGTGGCGAA ACCCGACAGG ACTATAAAGA TACCAGGCGT TTCCCCCTGG AAGCTCCCTC
      601 GTGCGCTCTC CTGTTCCGAC CCTGCCGCTT ACCGGATACC TGTCCGCCTT TCTCCCTTCG
      661 GGAAGCGTGG CGCTTTCTCA TAGCTCACGC TGTAGGTATC TCAGTTCGGT GTAGGTCGTT
      721 CGCTCCAAGC TGGGCTGTGT GCACGAACCC CCCGTTCAGC CCGACCGCTG CGCCTTATCC
      781 GGTAACTATC GTCTTGAGTC CAACCCGGTA AGACACGACT TATCGCCACT GGCAGCAGCC
      841 ACTGGTAACA GGATTAGCAG AGCGAGGTAT GTAGGCGGTG CTACAGAGTT CTTGAAGTGG
      901 TGGCCTAACT ACGGCTACAC TAGAAGAACA GTATTTGGTA TCTGCGCTCT GCTGAAGCCA
      961 GTTACCTTCG GAAAAAGAGT TGGTAGCTCT TGATCCGGCA AACAAACCAC CGCTGGTAGC
     1021 GGTGGTTTTT TTGTTTGCAA GCAGCAGATT ACGCGCAGAA AAAAAGGATC TCAAGAAGGC
     1081 CTACTATTAG CAACAACGAT CCTTTGATCT TTTCTACGGG GTCTGACGCT CAGTGGAACG
     1141 AAAACTCACG TTAAGGGATT TTGGTCATGA GATTATCAAA AAGGATCTTC ACCTAGATCC
     1201 TTTTAAATTA AAAATGAAGT TTTAAATCAA TCTAAAGTAT ATATGAGTAA ACTTGGTCTG
     1261 ACAGTTACCA ATGCTTAATC AGTGAGGCAC CTATCTCAGC GATCTGTCTA TTTCGTTCAT
     1321 CCATAGTTGC CTGACTCCCC GTCGTGTAGA TAACTACGAT ACGGGAGGGC TTACCATCTG
     1381 GCCCCAGTGC TGCAATGATA CCGCGAGAAC CACGCTCACC GGCTCCAGAT TTATCAGCAA
     1441 TAAACCAGCC AGCCGGAAGG GCCGAGCGCA GAAGTGGTCC TGCAACTTTA TCCGCCTCCA
     1501 TCCAGTCTAT TAATTGTTGC CGGGAAGCTA GAGTAAGTAG TTCGCCAGTT AATAGTTTGC
     1561 GCAACGTTGT TGCCATTGCT ACAGGCATCG TGGTGTCACG CTCGTCGTTT GGTATGGCTT
     1621 CATTCAGCTC CGGTTCCCAA CGATCAAGGC GAGTTACATG ATCCCCCATG TTGTGCAAAA
     1681 AAGCGGTTAG CTCCTTCGGT CCTCCGATCG TTGTCAGAAG TAAGTTGGCC GCAGTGTTAT
     1741 CACTCATGGT TATGGCAGCA CTGCATAATT CTCTTACTGT CATGCCATCC GTAAGATGCT
     1801 TTTCTGTGAC TGGTGAGTAC TCAACCAAGT CATTCTGAGA ATAGTGTATG CGGCGACCGA
     1861 GTTGCTCTTG CCCGGCGTCA ATACGGGATA ATACCGCGCC ACATAGCAGA ACTTTAAAAG
     1921 TGCTCATCAT TGGAAAACGT TCTTCGGGGC GAAAACTCTC AAGGATCTTA CCGCTGTTGA
     1981 GATCCAGTTC GATGTAACCC ACTCGTGCAC CCAACTGATC TTCAGCATCT TTTACTTTCA
     2041 CCAGCGTTTC TGGGTGAGCA AAAACAGGAA GGCAAAATGC CGCAAAAAAG GGAATAAGGG
     2101 CGACACGGAA ATGTTGAATA CTCATACTCT TCCTTTTTCA ATATTATTGA AGCATTTATC
     2161 AGGGTTATTG TCTCATGAGC GGATACATAT TTGAATGTAT TTAGAAAAAT AAACAAATAG
     2221 GGGTTCCGCG CACCTGCACC AGTCAGTAAA ACGACGGCCA GTAGTCAAAA GCCTCCGACC
     2281 GGAGGCTTTT GACTTGGTTC AGGTGGAGTG GGAGAACGAT GATGCTCACT CTCGCCGCCT
     2341 TTCCAAAGTG AGTCCTTCGT CTGTGACTAA CTGTGCCAAA TCGTCTTGCA AACTCCTGAT
     2401 CCAGTTTAAC TCACCAAATT ATAGCCATAC AGACGGTCTC A
//