LOCUS BBF10K_003409 2425 bp DNA circular 29-Apr-2025 ACCESSION BBF10K_003409 VERSION BBF10K_003409.1 FEATURES Location/Qualifiers misc_feature 5..105 /label=BT2_RiboJ_B34 CDS complement(1248..2107) /label=AmpR promoter complement(2109..2212) /label=AmpR promoter primer_bind 2231..2247 /label=M13 fwd primer_bind complement(386..401) /label=M13 rev rep_origin complement(469..1056) /label=ColE1 primer_bind 303..321 /label=SLiCE-for primer_bind complement(2275..2292) /label=SLiCE-rev misc_feature 1060..1249 /label=spacer CDS 420..470 /label=spacer terminator 336..383 /label=T7 terminator terminator 2248..2279 /label=tonB terminator misc_feature 298..2298 /label=pOpen_v3 backbone misc_feature 2419..2424 /label=BsaI_site misc_feature complement(111..116) /label=BsaI_site misc_feature 1..4 /label=TACT misc_feature 106..109 /label=GTCA ORIGIN 1 TACTAGCTGT CACCGGATGT GCTTTCCGGT CTGATGAGTC CGTGAGGACG AAACAGCCTC 61 TACAAATAAT TTTGTTTAAA CTAGAAAAGA GGAGAAATAC TAGATGTCAC GAGACCCGAT 121 TGCTTTCTCA ATCTCCGAGC GATTTAGCGT GACAGCCCCA GGGAACCCAC AAAATGTGAT 181 CGCAGTCCAT CCGATCGTAC ACAGAAAGGA AGGTCCACGG CTGGCTGTAA GAATACTGGC 241 CGTATCTCAA ACTGCACTTA GGGATAACAG GGTAATGCCA CGCTGACTGC CGTCACGCGC 301 TGAGGTGTCA ATCGTCGGAG CCGCTGAGCA ATAACTAGCA TAACCCCTTG GGGCCTCTAA 361 ACGGGTCTTG AGGGGTTTTT TGCATGGTCA TAGCTGTTTC CTGAGAGCTT GGCAGGTGAT 421 GACACACATT AACAAATTTC GTGAGGAGTC TCCAGAAGAA TGCCATTAAT TTCCATAGGC 481 TCCGCCCCCC TGACGAGCAT CACAAAAATC GACGCTCAAG TCAGAGGTGG CGAAACCCGA 541 CAGGACTATA AAGATACCAG GCGTTTCCCC CTGGAAGCTC CCTCGTGCGC TCTCCTGTTC 601 CGACCCTGCC GCTTACCGGA TACCTGTCCG CCTTTCTCCC TTCGGGAAGC GTGGCGCTTT 661 CTCATAGCTC ACGCTGTAGG TATCTCAGTT CGGTGTAGGT CGTTCGCTCC AAGCTGGGCT 721 GTGTGCACGA ACCCCCCGTT CAGCCCGACC GCTGCGCCTT ATCCGGTAAC TATCGTCTTG 781 AGTCCAACCC GGTAAGACAC GACTTATCGC CACTGGCAGC AGCCACTGGT AACAGGATTA 841 GCAGAGCGAG GTATGTAGGC GGTGCTACAG AGTTCTTGAA GTGGTGGCCT AACTACGGCT 901 ACACTAGAAG AACAGTATTT GGTATCTGCG CTCTGCTGAA GCCAGTTACC TTCGGAAAAA 961 GAGTTGGTAG CTCTTGATCC GGCAAACAAA CCACCGCTGG TAGCGGTGGT TTTTTTGTTT 1021 GCAAGCAGCA GATTACGCGC AGAAAAAAAG GATCTCAAGA AGGCCTACTA TTAGCAACAA 1081 CGATCCTTTG ATCTTTTCTA CGGGGTCTGA CGCTCAGTGG AACGAAAACT CACGTTAAGG 1141 GATTTTGGTC ATGAGATTAT CAAAAAGGAT CTTCACCTAG ATCCTTTTAA ATTAAAAATG 1201 AAGTTTTAAA TCAATCTAAA GTATATATGA GTAAACTTGG TCTGACAGTT ACCAATGCTT 1261 AATCAGTGAG GCACCTATCT CAGCGATCTG TCTATTTCGT TCATCCATAG TTGCCTGACT 1321 CCCCGTCGTG TAGATAACTA CGATACGGGA GGGCTTACCA TCTGGCCCCA GTGCTGCAAT 1381 GATACCGCGA GAACCACGCT CACCGGCTCC AGATTTATCA GCAATAAACC AGCCAGCCGG 1441 AAGGGCCGAG CGCAGAAGTG GTCCTGCAAC TTTATCCGCC TCCATCCAGT CTATTAATTG 1501 TTGCCGGGAA GCTAGAGTAA GTAGTTCGCC AGTTAATAGT TTGCGCAACG TTGTTGCCAT 1561 TGCTACAGGC ATCGTGGTGT CACGCTCGTC GTTTGGTATG GCTTCATTCA GCTCCGGTTC 1621 CCAACGATCA AGGCGAGTTA CATGATCCCC CATGTTGTGC AAAAAAGCGG TTAGCTCCTT 1681 CGGTCCTCCG ATCGTTGTCA GAAGTAAGTT GGCCGCAGTG TTATCACTCA TGGTTATGGC 1741 AGCACTGCAT AATTCTCTTA CTGTCATGCC ATCCGTAAGA TGCTTTTCTG TGACTGGTGA 1801 GTACTCAACC AAGTCATTCT GAGAATAGTG TATGCGGCGA CCGAGTTGCT CTTGCCCGGC 1861 GTCAATACGG GATAATACCG CGCCACATAG CAGAACTTTA AAAGTGCTCA TCATTGGAAA 1921 ACGTTCTTCG GGGCGAAAAC TCTCAAGGAT CTTACCGCTG TTGAGATCCA GTTCGATGTA 1981 ACCCACTCGT GCACCCAACT GATCTTCAGC ATCTTTTACT TTCACCAGCG TTTCTGGGTG 2041 AGCAAAAACA GGAAGGCAAA ATGCCGCAAA AAAGGGAATA AGGGCGACAC GGAAATGTTG 2101 AATACTCATA CTCTTCCTTT TTCAATATTA TTGAAGCATT TATCAGGGTT ATTGTCTCAT 2161 GAGCGGATAC ATATTTGAAT GTATTTAGAA AAATAAACAA ATAGGGGTTC CGCGCACCTG 2221 CACCAGTCAG TAAAACGACG GCCAGTAGTC AAAAGCCTCC GACCGGAGGC TTTTGACTTG 2281 GTTCAGGTGG AGTGGGAGAA CGATGATGCT CACTCTCGCA CGTCTACCGC GGTGCTCAAT 2341 GACCGGGACT AAAGAGGCGA AGATTATGGT GTGTGACCCG TTATGCTCGA GTTCGGTCAG 2401 AGCGTCATTG CGAGTAGTGG TCTCA //