LOCUS       BBF10K_003410           2444 bp    DNA     circular     17-Jun-2025
ACCESSION   BBF10K_003410
VERSION     BBF10K_003410.1  
FEATURES             Location/Qualifiers
     misc_feature    5..25
                     /label=BC_B0034
     CDS             complement(1218..2077)
                     /label=AmpR
     promoter        complement(2079..2182)
                     /label=AmpR promoter
     primer_bind     2201..2217
                     /label=M13 fwd
     primer_bind     complement(356..371)
                     /label=M13 rev
     rep_origin      complement(439..1026)
                     /label=ColE1
     primer_bind     273..291
                     /label=SLiCE-for
     primer_bind     complement(2245..2262)
                     /label=SLiCE-rev
     misc_feature    1030..1219
                     /label=spacer
     CDS             390..440
                     /label=spacer
     terminator      306..353
                     /label=T7 terminator
     terminator      2218..2249
                     /label=tonB terminator
     misc_feature    268..2268
                     /label=pOpen_v3 backbone
     misc_feature    2438..2443
                     /label=BsaI_site
     misc_feature    complement(31..36)
                     /label=BsaI_site
     misc_feature    1..4
                     /label=TACT
     misc_feature    26..29
                     /label=AATG
ORIGIN
        1 TACTAGAGAA AGAGGAGAAA TACTAAATGC GAGACCTATA AACGCCAGGT TGTATCCGCA
       61 TTTGATGCTA CCATGGATGA GTCAGCGTCG AGCACGCGGC ATTTATTGCA TGAGTAGGGT
      121 TGACTAAGAA CCGTTAGATG CCTCGCTGTA CTAATAATTG TCAACAGATC GTCAAGATTA
      181 GAAAATAGGG TTTAGTCCGG CAATACTTAC TTGCACAGTA TCTTAAGATA GGGATAACAG
      241 GGTAATGGGA TTAAGAAAAT CACGGTCCGC TGAGGTGTCA ATCGTCGGAG CCGCTGAGCA
      301 ATAACTAGCA TAACCCCTTG GGGCCTCTAA ACGGGTCTTG AGGGGTTTTT TGCATGGTCA
      361 TAGCTGTTTC CTGAGAGCTT GGCAGGTGAT GACACACATT AACAAATTTC GTGAGGAGTC
      421 TCCAGAAGAA TGCCATTAAT TTCCATAGGC TCCGCCCCCC TGACGAGCAT CACAAAAATC
      481 GACGCTCAAG TCAGAGGTGG CGAAACCCGA CAGGACTATA AAGATACCAG GCGTTTCCCC
      541 CTGGAAGCTC CCTCGTGCGC TCTCCTGTTC CGACCCTGCC GCTTACCGGA TACCTGTCCG
      601 CCTTTCTCCC TTCGGGAAGC GTGGCGCTTT CTCATAGCTC ACGCTGTAGG TATCTCAGTT
      661 CGGTGTAGGT CGTTCGCTCC AAGCTGGGCT GTGTGCACGA ACCCCCCGTT CAGCCCGACC
      721 GCTGCGCCTT ATCCGGTAAC TATCGTCTTG AGTCCAACCC GGTAAGACAC GACTTATCGC
      781 CACTGGCAGC AGCCACTGGT AACAGGATTA GCAGAGCGAG GTATGTAGGC GGTGCTACAG
      841 AGTTCTTGAA GTGGTGGCCT AACTACGGCT ACACTAGAAG AACAGTATTT GGTATCTGCG
      901 CTCTGCTGAA GCCAGTTACC TTCGGAAAAA GAGTTGGTAG CTCTTGATCC GGCAAACAAA
      961 CCACCGCTGG TAGCGGTGGT TTTTTTGTTT GCAAGCAGCA GATTACGCGC AGAAAAAAAG
     1021 GATCTCAAGA AGGCCTACTA TTAGCAACAA CGATCCTTTG ATCTTTTCTA CGGGGTCTGA
     1081 CGCTCAGTGG AACGAAAACT CACGTTAAGG GATTTTGGTC ATGAGATTAT CAAAAAGGAT
     1141 CTTCACCTAG ATCCTTTTAA ATTAAAAATG AAGTTTTAAA TCAATCTAAA GTATATATGA
     1201 GTAAACTTGG TCTGACAGTT ACCAATGCTT AATCAGTGAG GCACCTATCT CAGCGATCTG
     1261 TCTATTTCGT TCATCCATAG TTGCCTGACT CCCCGTCGTG TAGATAACTA CGATACGGGA
     1321 GGGCTTACCA TCTGGCCCCA GTGCTGCAAT GATACCGCGA GAACCACGCT CACCGGCTCC
     1381 AGATTTATCA GCAATAAACC AGCCAGCCGG AAGGGCCGAG CGCAGAAGTG GTCCTGCAAC
     1441 TTTATCCGCC TCCATCCAGT CTATTAATTG TTGCCGGGAA GCTAGAGTAA GTAGTTCGCC
     1501 AGTTAATAGT TTGCGCAACG TTGTTGCCAT TGCTACAGGC ATCGTGGTGT CACGCTCGTC
     1561 GTTTGGTATG GCTTCATTCA GCTCCGGTTC CCAACGATCA AGGCGAGTTA CATGATCCCC
     1621 CATGTTGTGC AAAAAAGCGG TTAGCTCCTT CGGTCCTCCG ATCGTTGTCA GAAGTAAGTT
     1681 GGCCGCAGTG TTATCACTCA TGGTTATGGC AGCACTGCAT AATTCTCTTA CTGTCATGCC
     1741 ATCCGTAAGA TGCTTTTCTG TGACTGGTGA GTACTCAACC AAGTCATTCT GAGAATAGTG
     1801 TATGCGGCGA CCGAGTTGCT CTTGCCCGGC GTCAATACGG GATAATACCG CGCCACATAG
     1861 CAGAACTTTA AAAGTGCTCA TCATTGGAAA ACGTTCTTCG GGGCGAAAAC TCTCAAGGAT
     1921 CTTACCGCTG TTGAGATCCA GTTCGATGTA ACCCACTCGT GCACCCAACT GATCTTCAGC
     1981 ATCTTTTACT TTCACCAGCG TTTCTGGGTG AGCAAAAACA GGAAGGCAAA ATGCCGCAAA
     2041 AAAGGGAATA AGGGCGACAC GGAAATGTTG AATACTCATA CTCTTCCTTT TTCAATATTA
     2101 TTGAAGCATT TATCAGGGTT ATTGTCTCAT GAGCGGATAC ATATTTGAAT GTATTTAGAA
     2161 AAATAAACAA ATAGGGGTTC CGCGCACCTG CACCAGTCAG TAAAACGACG GCCAGTAGTC
     2221 AAAAGCCTCC GACCGGAGGC TTTTGACTTG GTTCAGGTGG AGTGGGAGAA CGATGATGCT
     2281 CACTCTCGGG TACCAGCATT TTCGGAGGTT CTCTAACAGT ATGGATAACC GTGTTTTCAC
     2341 TGTGCTGCGG TTACCCATCG CCTGAAATCC AGTTGGTGTC AAGCCATTCC CTGTCTAGGA
     2401 CGCCGCATGT AGTAAAACAT ATACATTGCT CGGGTTCGGT CTCA
//