LOCUS       BBF10K_003411           2446 bp    DNA     circular     29-Apr-2025
ACCESSION   BBF10K_003411
VERSION     BBF10K_003411.1  
FEATURES             Location/Qualifiers
     misc_feature    5..26
                     /label=BC_B0032
     CDS             complement(1219..2078)
                     /label=AmpR
     promoter        complement(2080..2183)
                     /label=AmpR promoter
     primer_bind     2202..2218
                     /label=M13 fwd
     primer_bind     complement(357..372)
                     /label=M13 rev
     rep_origin      complement(440..1027)
                     /label=ColE1
     primer_bind     274..292
                     /label=SLiCE-for
     primer_bind     complement(2246..2263)
                     /label=SLiCE-rev
     misc_feature    1031..1220
                     /label=spacer
     CDS             391..441
                     /label=spacer
     terminator      307..354
                     /label=T7 terminator
     terminator      2219..2250
                     /label=tonB terminator
     misc_feature    269..2269
                     /label=pOpen_v3 backbone
     misc_feature    152..157
                     /label=BsaI_site
     misc_feature    complement(32..37)
                     /label=BsaI_site
     misc_feature    1..4
                     /label=TACT
     misc_feature    27..30
                     /label=AATG
ORIGIN
        1 TACTAGAGTC ACACAGGAAA GTACTAAATG CGAGACCTTG AAGAGGCCAC TTAAAAATGG
       61 CAGTGGTTGA TGCTCTAAAC TCCATTTGGT TAATTCGTGT ATCACCGCGA TAGGCTGATA
      121 AAGGTTTAAT ATTGTATAGC AAGGTACTTC CGGTCTCAAT GAATGGCCGG GAAAGGTACG
      181 CGCGTGGAGT ACGTCTGAAC TTGGGACTAG GTTGCCTCAT TGGTAAGTGT AGGGATAACA
      241 GGGTAATGCG TGGATTTCGA GCGGTTCTCG CTGAGGTGTC AATCGTCGGA GCCGCTGAGC
      301 AATAACTAGC ATAACCCCTT GGGGCCTCTA AACGGGTCTT GAGGGGTTTT TTGCATGGTC
      361 ATAGCTGTTT CCTGAGAGCT TGGCAGGTGA TGACACACAT TAACAAATTT CGTGAGGAGT
      421 CTCCAGAAGA ATGCCATTAA TTTCCATAGG CTCCGCCCCC CTGACGAGCA TCACAAAAAT
      481 CGACGCTCAA GTCAGAGGTG GCGAAACCCG ACAGGACTAT AAAGATACCA GGCGTTTCCC
      541 CCTGGAAGCT CCCTCGTGCG CTCTCCTGTT CCGACCCTGC CGCTTACCGG ATACCTGTCC
      601 GCCTTTCTCC CTTCGGGAAG CGTGGCGCTT TCTCATAGCT CACGCTGTAG GTATCTCAGT
      661 TCGGTGTAGG TCGTTCGCTC CAAGCTGGGC TGTGTGCACG AACCCCCCGT TCAGCCCGAC
      721 CGCTGCGCCT TATCCGGTAA CTATCGTCTT GAGTCCAACC CGGTAAGACA CGACTTATCG
      781 CCACTGGCAG CAGCCACTGG TAACAGGATT AGCAGAGCGA GGTATGTAGG CGGTGCTACA
      841 GAGTTCTTGA AGTGGTGGCC TAACTACGGC TACACTAGAA GAACAGTATT TGGTATCTGC
      901 GCTCTGCTGA AGCCAGTTAC CTTCGGAAAA AGAGTTGGTA GCTCTTGATC CGGCAAACAA
      961 ACCACCGCTG GTAGCGGTGG TTTTTTTGTT TGCAAGCAGC AGATTACGCG CAGAAAAAAA
     1021 GGATCTCAAG AAGGCCTACT ATTAGCAACA ACGATCCTTT GATCTTTTCT ACGGGGTCTG
     1081 ACGCTCAGTG GAACGAAAAC TCACGTTAAG GGATTTTGGT CATGAGATTA TCAAAAAGGA
     1141 TCTTCACCTA GATCCTTTTA AATTAAAAAT GAAGTTTTAA ATCAATCTAA AGTATATATG
     1201 AGTAAACTTG GTCTGACAGT TACCAATGCT TAATCAGTGA GGCACCTATC TCAGCGATCT
     1261 GTCTATTTCG TTCATCCATA GTTGCCTGAC TCCCCGTCGT GTAGATAACT ACGATACGGG
     1321 AGGGCTTACC ATCTGGCCCC AGTGCTGCAA TGATACCGCG AGAACCACGC TCACCGGCTC
     1381 CAGATTTATC AGCAATAAAC CAGCCAGCCG GAAGGGCCGA GCGCAGAAGT GGTCCTGCAA
     1441 CTTTATCCGC CTCCATCCAG TCTATTAATT GTTGCCGGGA AGCTAGAGTA AGTAGTTCGC
     1501 CAGTTAATAG TTTGCGCAAC GTTGTTGCCA TTGCTACAGG CATCGTGGTG TCACGCTCGT
     1561 CGTTTGGTAT GGCTTCATTC AGCTCCGGTT CCCAACGATC AAGGCGAGTT ACATGATCCC
     1621 CCATGTTGTG CAAAAAAGCG GTTAGCTCCT TCGGTCCTCC GATCGTTGTC AGAAGTAAGT
     1681 TGGCCGCAGT GTTATCACTC ATGGTTATGG CAGCACTGCA TAATTCTCTT ACTGTCATGC
     1741 CATCCGTAAG ATGCTTTTCT GTGACTGGTG AGTACTCAAC CAAGTCATTC TGAGAATAGT
     1801 GTATGCGGCG ACCGAGTTGC TCTTGCCCGG CGTCAATACG GGATAATACC GCGCCACATA
     1861 GCAGAACTTT AAAAGTGCTC ATCATTGGAA AACGTTCTTC GGGGCGAAAA CTCTCAAGGA
     1921 TCTTACCGCT GTTGAGATCC AGTTCGATGT AACCCACTCG TGCACCCAAC TGATCTTCAG
     1981 CATCTTTTAC TTTCACCAGC GTTTCTGGGT GAGCAAAAAC AGGAAGGCAA AATGCCGCAA
     2041 AAAAGGGAAT AAGGGCGACA CGGAAATGTT GAATACTCAT ACTCTTCCTT TTTCAATATT
     2101 ATTGAAGCAT TTATCAGGGT TATTGTCTCA TGAGCGGATA CATATTTGAA TGTATTTAGA
     2161 AAAATAAACA AATAGGGGTT CCGCGCACCT GCACCAGTCA GTAAAACGAC GGCCAGTAGT
     2221 CAAAAGCCTC CGACCGGAGG CTTTTGACTT GGTTCAGGTG GAGTGGGAGA ACGATGATGC
     2281 TCACTCTCGT ATGGGAGGAT CAAGGAGCCA ATAGAAAGGC TTCTTTCTCA CTCGCTAGGA
     2341 GGCAATTGTA TAACAATGCT TACTATATCG ATACATAAAA CATGTCCATC GGTTGCCCAA
     2401 ACTGTAAAGT GTCTATCACC CCTAGGCCCG TTTCCCGCAG GTCTCA
//