LOCUS       BBF10K_003412           2416 bp    DNA     circular     29-Apr-2025
ACCESSION   BBF10K_003412
VERSION     BBF10K_003412.1  
FEATURES             Location/Qualifiers
     misc_feature    5..104
                     /label=BC_RiboJ_RBS64
     CDS             complement(1243..2102)
                     /label=AmpR
     promoter        complement(2104..2207)
                     /label=AmpR promoter
     primer_bind     2226..2242
                     /label=M13 fwd
     primer_bind     complement(381..396)
                     /label=M13 rev
     rep_origin      complement(464..1051)
                     /label=ColE1
     primer_bind     298..316
                     /label=SLiCE-for
     primer_bind     complement(2270..2287)
                     /label=SLiCE-rev
     misc_feature    1055..1244
                     /label=spacer
     CDS             415..465
                     /label=spacer
     terminator      331..378
                     /label=T7 terminator
     terminator      2243..2274
                     /label=tonB terminator
     misc_feature    293..2293
                     /label=pOpen_v3 backbone
     misc_feature    2410..2415
                     /label=BsaI_site
     misc_feature    complement(110..115)
                     /label=BsaI_site
     misc_feature    1..4
                     /label=TACT
     misc_feature    105..108
                     /label=AATG
ORIGIN
        1 TACTAGCTGT CACCGGATGT GCTTTCCGGT CTGATGAGTC CGTGAGGACG AAACAGCCTC
       61 TACAAATAAT TTTGTTTAAT ACTAGAGAAA GAGGGGAAAT ACTAAATGCG AGACCTCTCA
      121 TGACTGAACT CGCAAGGGTC GTGATGTCGG TTCCTTCAAA GGTTAAAAAA CAAAGGCTTA
      181 CTGTGCACAG AGGAACGCCC ATTTAGCGGC TAGTGTCACA TTGTGTCGGA CTACGCTGGT
      241 TTCTCCCTCA TCCTAGGGAT AACAGGGTAA TGCACTCAGT CGAATCTAAA AACGCTGAGG
      301 TGTCAATCGT CGGAGCCGCT GAGCAATAAC TAGCATAACC CCTTGGGGCC TCTAAACGGG
      361 TCTTGAGGGG TTTTTTGCAT GGTCATAGCT GTTTCCTGAG AGCTTGGCAG GTGATGACAC
      421 ACATTAACAA ATTTCGTGAG GAGTCTCCAG AAGAATGCCA TTAATTTCCA TAGGCTCCGC
      481 CCCCCTGACG AGCATCACAA AAATCGACGC TCAAGTCAGA GGTGGCGAAA CCCGACAGGA
      541 CTATAAAGAT ACCAGGCGTT TCCCCCTGGA AGCTCCCTCG TGCGCTCTCC TGTTCCGACC
      601 CTGCCGCTTA CCGGATACCT GTCCGCCTTT CTCCCTTCGG GAAGCGTGGC GCTTTCTCAT
      661 AGCTCACGCT GTAGGTATCT CAGTTCGGTG TAGGTCGTTC GCTCCAAGCT GGGCTGTGTG
      721 CACGAACCCC CCGTTCAGCC CGACCGCTGC GCCTTATCCG GTAACTATCG TCTTGAGTCC
      781 AACCCGGTAA GACACGACTT ATCGCCACTG GCAGCAGCCA CTGGTAACAG GATTAGCAGA
      841 GCGAGGTATG TAGGCGGTGC TACAGAGTTC TTGAAGTGGT GGCCTAACTA CGGCTACACT
      901 AGAAGAACAG TATTTGGTAT CTGCGCTCTG CTGAAGCCAG TTACCTTCGG AAAAAGAGTT
      961 GGTAGCTCTT GATCCGGCAA ACAAACCACC GCTGGTAGCG GTGGTTTTTT TGTTTGCAAG
     1021 CAGCAGATTA CGCGCAGAAA AAAAGGATCT CAAGAAGGCC TACTATTAGC AACAACGATC
     1081 CTTTGATCTT TTCTACGGGG TCTGACGCTC AGTGGAACGA AAACTCACGT TAAGGGATTT
     1141 TGGTCATGAG ATTATCAAAA AGGATCTTCA CCTAGATCCT TTTAAATTAA AAATGAAGTT
     1201 TTAAATCAAT CTAAAGTATA TATGAGTAAA CTTGGTCTGA CAGTTACCAA TGCTTAATCA
     1261 GTGAGGCACC TATCTCAGCG ATCTGTCTAT TTCGTTCATC CATAGTTGCC TGACTCCCCG
     1321 TCGTGTAGAT AACTACGATA CGGGAGGGCT TACCATCTGG CCCCAGTGCT GCAATGATAC
     1381 CGCGAGAACC ACGCTCACCG GCTCCAGATT TATCAGCAAT AAACCAGCCA GCCGGAAGGG
     1441 CCGAGCGCAG AAGTGGTCCT GCAACTTTAT CCGCCTCCAT CCAGTCTATT AATTGTTGCC
     1501 GGGAAGCTAG AGTAAGTAGT TCGCCAGTTA ATAGTTTGCG CAACGTTGTT GCCATTGCTA
     1561 CAGGCATCGT GGTGTCACGC TCGTCGTTTG GTATGGCTTC ATTCAGCTCC GGTTCCCAAC
     1621 GATCAAGGCG AGTTACATGA TCCCCCATGT TGTGCAAAAA AGCGGTTAGC TCCTTCGGTC
     1681 CTCCGATCGT TGTCAGAAGT AAGTTGGCCG CAGTGTTATC ACTCATGGTT ATGGCAGCAC
     1741 TGCATAATTC TCTTACTGTC ATGCCATCCG TAAGATGCTT TTCTGTGACT GGTGAGTACT
     1801 CAACCAAGTC ATTCTGAGAA TAGTGTATGC GGCGACCGAG TTGCTCTTGC CCGGCGTCAA
     1861 TACGGGATAA TACCGCGCCA CATAGCAGAA CTTTAAAAGT GCTCATCATT GGAAAACGTT
     1921 CTTCGGGGCG AAAACTCTCA AGGATCTTAC CGCTGTTGAG ATCCAGTTCG ATGTAACCCA
     1981 CTCGTGCACC CAACTGATCT TCAGCATCTT TTACTTTCAC CAGCGTTTCT GGGTGAGCAA
     2041 AAACAGGAAG GCAAAATGCC GCAAAAAAGG GAATAAGGGC GACACGGAAA TGTTGAATAC
     2101 TCATACTCTT CCTTTTTCAA TATTATTGAA GCATTTATCA GGGTTATTGT CTCATGAGCG
     2161 GATACATATT TGAATGTATT TAGAAAAATA AACAAATAGG GGTTCCGCGC ACCTGCACCA
     2221 GTCAGTAAAA CGACGGCCAG TAGTCAAAAG CCTCCGACCG GAGGCTTTTG ACTTGGTTCA
     2281 GGTGGAGTGG GAGAACGATG ATGCTCACTC TCGGGCGTCT TGAATCCTCG GTCCCCTTTG
     2341 TCTTTCCAGA TTAATTCATT TCCCTCATTC ACAAGCTTAC CAAGTCAACA TTGGTATATG
     2401 AATGCGACCG GTCTCA
//