LOCUS       BBF10K_003413           2404 bp    DNA     circular     29-Apr-2025
ACCESSION   BBF10K_003413
VERSION     BBF10K_003413.1  
FEATURES             Location/Qualifiers
     misc_feature    5..102
                     /label=BC_RiboJ_B34
     CDS             complement(1231..2090)
                     /label=AmpR
     promoter        complement(2092..2195)
                     /label=AmpR promoter
     primer_bind     2214..2230
                     /label=M13 fwd
     primer_bind     complement(369..384)
                     /label=M13 rev
     rep_origin      complement(452..1039)
                     /label=ColE1
     primer_bind     286..304
                     /label=SLiCE-for
     primer_bind     complement(2258..2275)
                     /label=SLiCE-rev
     misc_feature    1043..1232
                     /label=spacer
     CDS             403..453
                     /label=spacer
     terminator      319..366
                     /label=T7 terminator
     terminator      2231..2262
                     /label=tonB terminator
     misc_feature    281..2281
                     /label=pOpen_v3 backbone
     misc_feature    2398..2403
                     /label=BsaI_site
     misc_feature    complement(108..113)
                     /label=BsaI_site
     misc_feature    1..4
                     /label=TACT
     misc_feature    103..106
                     /label=AATG
ORIGIN
        1 TACTAGCTGT CACCGGATGT GCTTTCCGGT CTGATGAGTC CGTGAGGACG AAACAGCCTC
       61 TACAAATAAT TTTGTTTAAA CTAGAAAAGA GGAGAAATAC TAAATGCGAG ACCAGGGCTA
      121 GAATTACTTA CCGGCCTTCA CCATGCCTGC GCTATACGCG CCCACTCTCC CGTTTATCCG
      181 TCCAAGCGGA TGCAATGCGA GAGGATTCGG TCAGTTCACT TCGGGATGCT AGTCGTATTC
      241 ATAGGGATAA CAGGGTAATG GACTAGGTGC TAGAGTCTGT CGCTGAGGTG TCAATCGTCG
      301 GAGCCGCTGA GCAATAACTA GCATAACCCC TTGGGGCCTC TAAACGGGTC TTGAGGGGTT
      361 TTTTGCATGG TCATAGCTGT TTCCTGAGAG CTTGGCAGGT GATGACACAC ATTAACAAAT
      421 TTCGTGAGGA GTCTCCAGAA GAATGCCATT AATTTCCATA GGCTCCGCCC CCCTGACGAG
      481 CATCACAAAA ATCGACGCTC AAGTCAGAGG TGGCGAAACC CGACAGGACT ATAAAGATAC
      541 CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG TTCCGACCCT GCCGCTTACC
      601 GGATACCTGT CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC TTTCTCATAG CTCACGCTGT
      661 AGGTATCTCA GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG GCTGTGTGCA CGAACCCCCC
      721 GTTCAGCCCG ACCGCTGCGC CTTATCCGGT AACTATCGTC TTGAGTCCAA CCCGGTAAGA
      781 CACGACTTAT CGCCACTGGC AGCAGCCACT GGTAACAGGA TTAGCAGAGC GAGGTATGTA
      841 GGCGGTGCTA CAGAGTTCTT GAAGTGGTGG CCTAACTACG GCTACACTAG AAGAACAGTA
      901 TTTGGTATCT GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA AAAGAGTTGG TAGCTCTTGA
      961 TCCGGCAAAC AAACCACCGC TGGTAGCGGT GGTTTTTTTG TTTGCAAGCA GCAGATTACG
     1021 CGCAGAAAAA AAGGATCTCA AGAAGGCCTA CTATTAGCAA CAACGATCCT TTGATCTTTT
     1081 CTACGGGGTC TGACGCTCAG TGGAACGAAA ACTCACGTTA AGGGATTTTG GTCATGAGAT
     1141 TATCAAAAAG GATCTTCACC TAGATCCTTT TAAATTAAAA ATGAAGTTTT AAATCAATCT
     1201 AAAGTATATA TGAGTAAACT TGGTCTGACA GTTACCAATG CTTAATCAGT GAGGCACCTA
     1261 TCTCAGCGAT CTGTCTATTT CGTTCATCCA TAGTTGCCTG ACTCCCCGTC GTGTAGATAA
     1321 CTACGATACG GGAGGGCTTA CCATCTGGCC CCAGTGCTGC AATGATACCG CGAGAACCAC
     1381 GCTCACCGGC TCCAGATTTA TCAGCAATAA ACCAGCCAGC CGGAAGGGCC GAGCGCAGAA
     1441 GTGGTCCTGC AACTTTATCC GCCTCCATCC AGTCTATTAA TTGTTGCCGG GAAGCTAGAG
     1501 TAAGTAGTTC GCCAGTTAAT AGTTTGCGCA ACGTTGTTGC CATTGCTACA GGCATCGTGG
     1561 TGTCACGCTC GTCGTTTGGT ATGGCTTCAT TCAGCTCCGG TTCCCAACGA TCAAGGCGAG
     1621 TTACATGATC CCCCATGTTG TGCAAAAAAG CGGTTAGCTC CTTCGGTCCT CCGATCGTTG
     1681 TCAGAAGTAA GTTGGCCGCA GTGTTATCAC TCATGGTTAT GGCAGCACTG CATAATTCTC
     1741 TTACTGTCAT GCCATCCGTA AGATGCTTTT CTGTGACTGG TGAGTACTCA ACCAAGTCAT
     1801 TCTGAGAATA GTGTATGCGG CGACCGAGTT GCTCTTGCCC GGCGTCAATA CGGGATAATA
     1861 CCGCGCCACA TAGCAGAACT TTAAAAGTGC TCATCATTGG AAAACGTTCT TCGGGGCGAA
     1921 AACTCTCAAG GATCTTACCG CTGTTGAGAT CCAGTTCGAT GTAACCCACT CGTGCACCCA
     1981 ACTGATCTTC AGCATCTTTT ACTTTCACCA GCGTTTCTGG GTGAGCAAAA ACAGGAAGGC
     2041 AAAATGCCGC AAAAAAGGGA ATAAGGGCGA CACGGAAATG TTGAATACTC ATACTCTTCC
     2101 TTTTTCAATA TTATTGAAGC ATTTATCAGG GTTATTGTCT CATGAGCGGA TACATATTTG
     2161 AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC CTGCACCAGT CAGTAAAACG
     2221 ACGGCCAGTA GTCAAAAGCC TCCGACCGGA GGCTTTTGAC TTGGTTCAGG TGGAGTGGGA
     2281 GAACGATGAT GCTCACTCTC GGTTATTGGG GACTTACACA GGCGTAGACT ACAATGGGCC
     2341 CAACTCAATC ACAGCTCGAG CGCCTTGAAT AACGTACTCA TCTCTATACA TTCTCGAGGT
     2401 CTCA
//