LOCUS       BBF10K_003414           2406 bp    DNA     circular     17-Jun-2025
ACCESSION   BBF10K_003414
VERSION     BBF10K_003414.1  
FEATURES             Location/Qualifiers
     misc_feature    5..88
                     /label=BC_BCD23
     CDS             complement(1229..2088)
                     /label=AmpR
     promoter        complement(2090..2193)
                     /label=AmpR promoter
     primer_bind     2212..2228
                     /label=M13 fwd
     primer_bind     complement(367..382)
                     /label=M13 rev
     rep_origin      complement(450..1037)
                     /label=ColE1
     primer_bind     284..302
                     /label=SLiCE-for
     primer_bind     complement(2256..2273)
                     /label=SLiCE-rev
     misc_feature    1041..1230
                     /label=spacer
     CDS             401..451
                     /label=spacer
     terminator      317..364
                     /label=T7 terminator
     terminator      2229..2260
                     /label=tonB terminator
     misc_feature    279..2279
                     /label=pOpen_v3 backbone
     misc_feature    2400..2405
                     /label=BsaI_site
     misc_feature    complement(94..99)
                     /label=BsaI_site
     misc_feature    1..4
                     /label=TACT
     misc_feature    89..92
                     /label=AATG
ORIGIN
        1 TACTGGGCCC AAGTTCACTT AAAAAGGAGA TCAACAATGA AAGCAATTTT CGTACTGAAA
       61 CATCTTAATC ATGCGAAGGA CAGTTTCTAA TGCGAGACCT ACATGTTCGT TGGCCTTCCG
      121 ACACGAACCT CAGTTGGCCT ACATCCTACC TGAGGTCTGT GCCCCGGTGG TGAGAAGTGC
      181 GCATTTCGTT CTTGCAGAGG TACGGTTTAC AGACCACTTT AGTTGAGAAA AGTCCGACGT
      241 AGGGATAACA GGGTAATTAC AGGTGGGAAA CACCCTGCCG CTGAGGTGTC AATCGTCGGA
      301 GCCGCTGAGC AATAACTAGC ATAACCCCTT GGGGCCTCTA AACGGGTCTT GAGGGGTTTT
      361 TTGCATGGTC ATAGCTGTTT CCTGAGAGCT TGGCAGGTGA TGACACACAT TAACAAATTT
      421 CGTGAGGAGT CTCCAGAAGA ATGCCATTAA TTTCCATAGG CTCCGCCCCC CTGACGAGCA
      481 TCACAAAAAT CGACGCTCAA GTCAGAGGTG GCGAAACCCG ACAGGACTAT AAAGATACCA
      541 GGCGTTTCCC CCTGGAAGCT CCCTCGTGCG CTCTCCTGTT CCGACCCTGC CGCTTACCGG
      601 ATACCTGTCC GCCTTTCTCC CTTCGGGAAG CGTGGCGCTT TCTCATAGCT CACGCTGTAG
      661 GTATCTCAGT TCGGTGTAGG TCGTTCGCTC CAAGCTGGGC TGTGTGCACG AACCCCCCGT
      721 TCAGCCCGAC CGCTGCGCCT TATCCGGTAA CTATCGTCTT GAGTCCAACC CGGTAAGACA
      781 CGACTTATCG CCACTGGCAG CAGCCACTGG TAACAGGATT AGCAGAGCGA GGTATGTAGG
      841 CGGTGCTACA GAGTTCTTGA AGTGGTGGCC TAACTACGGC TACACTAGAA GAACAGTATT
      901 TGGTATCTGC GCTCTGCTGA AGCCAGTTAC CTTCGGAAAA AGAGTTGGTA GCTCTTGATC
      961 CGGCAAACAA ACCACCGCTG GTAGCGGTGG TTTTTTTGTT TGCAAGCAGC AGATTACGCG
     1021 CAGAAAAAAA GGATCTCAAG AAGGCCTACT ATTAGCAACA ACGATCCTTT GATCTTTTCT
     1081 ACGGGGTCTG ACGCTCAGTG GAACGAAAAC TCACGTTAAG GGATTTTGGT CATGAGATTA
     1141 TCAAAAAGGA TCTTCACCTA GATCCTTTTA AATTAAAAAT GAAGTTTTAA ATCAATCTAA
     1201 AGTATATATG AGTAAACTTG GTCTGACAGT TACCAATGCT TAATCAGTGA GGCACCTATC
     1261 TCAGCGATCT GTCTATTTCG TTCATCCATA GTTGCCTGAC TCCCCGTCGT GTAGATAACT
     1321 ACGATACGGG AGGGCTTACC ATCTGGCCCC AGTGCTGCAA TGATACCGCG AGAACCACGC
     1381 TCACCGGCTC CAGATTTATC AGCAATAAAC CAGCCAGCCG GAAGGGCCGA GCGCAGAAGT
     1441 GGTCCTGCAA CTTTATCCGC CTCCATCCAG TCTATTAATT GTTGCCGGGA AGCTAGAGTA
     1501 AGTAGTTCGC CAGTTAATAG TTTGCGCAAC GTTGTTGCCA TTGCTACAGG CATCGTGGTG
     1561 TCACGCTCGT CGTTTGGTAT GGCTTCATTC AGCTCCGGTT CCCAACGATC AAGGCGAGTT
     1621 ACATGATCCC CCATGTTGTG CAAAAAAGCG GTTAGCTCCT TCGGTCCTCC GATCGTTGTC
     1681 AGAAGTAAGT TGGCCGCAGT GTTATCACTC ATGGTTATGG CAGCACTGCA TAATTCTCTT
     1741 ACTGTCATGC CATCCGTAAG ATGCTTTTCT GTGACTGGTG AGTACTCAAC CAAGTCATTC
     1801 TGAGAATAGT GTATGCGGCG ACCGAGTTGC TCTTGCCCGG CGTCAATACG GGATAATACC
     1861 GCGCCACATA GCAGAACTTT AAAAGTGCTC ATCATTGGAA AACGTTCTTC GGGGCGAAAA
     1921 CTCTCAAGGA TCTTACCGCT GTTGAGATCC AGTTCGATGT AACCCACTCG TGCACCCAAC
     1981 TGATCTTCAG CATCTTTTAC TTTCACCAGC GTTTCTGGGT GAGCAAAAAC AGGAAGGCAA
     2041 AATGCCGCAA AAAAGGGAAT AAGGGCGACA CGGAAATGTT GAATACTCAT ACTCTTCCTT
     2101 TTTCAATATT ATTGAAGCAT TTATCAGGGT TATTGTCTCA TGAGCGGATA CATATTTGAA
     2161 TGTATTTAGA AAAATAAACA AATAGGGGTT CCGCGCACCT GCACCAGTCA GTAAAACGAC
     2221 GGCCAGTAGT CAAAAGCCTC CGACCGGAGG CTTTTGACTT GGTTCAGGTG GAGTGGGAGA
     2281 ACGATGATGC TCACTCTCGG CGGATTTTTA CAGATACTAA CCAGGTGATT TGAAGTAAAT
     2341 TAGTTGAGGA TTTAGCCGCG CTATCCGGTA ATCTCCAAAT TAAAACATAC CGTTCCATGG
     2401 GTCTCA
//