LOCUS BBF10K_003417 2419 bp DNA circular 17-Jun-2025 ACCESSION BBF10K_003417 VERSION BBF10K_003417.1 FEATURES Location/Qualifiers CDS 5..39 /label=T2C_GS-flexible CDS complement(1212..2071) /label=AmpR promoter complement(2073..2176) /label=AmpR promoter primer_bind 2195..2211 /label=M13 fwd primer_bind complement(350..365) /label=M13 rev rep_origin complement(433..1020) /label=ColE1 primer_bind 267..285 /label=SLiCE-for primer_bind complement(2239..2256) /label=SLiCE-rev misc_feature 1024..1213 /label=spacer CDS 384..434 /label=spacer terminator 300..347 /label=T7 terminator terminator 2212..2243 /label=tonB terminator misc_feature 262..2262 /label=pOpen_v3 backbone misc_feature 2413..2418 /label=BsaI_site misc_feature complement(45..50) /label=BsaI_site misc_feature 1..4 /label=GTCA misc_feature 40..43 /label=AATG ORIGIN 1 GTCAGGAGGT GGCTCTGGCG GTGGATCAGG TGGAGGCTCA ATGCGAGACC ACCTTATCAA 61 AGCTTGCTAC CAATAATTAG GATTATTGCC TTGCGACAGA CTTCCTACTC AGACTGCCTC 121 ACATTGAGCT AGTCAATGAG CGATAAGCTT GACCCGCTTT CTAGGGTCGC GAATACGTGA 181 AGCGGGTTAG ATACTGCAAC TTGAGTCAAA ATGGACGTGT TGTAGGGATA ACAGGGTAAT 241 TTACACTATT TACGAACGGC GCGCTGAGGT GTCAATCGTC GGAGCCGCTG AGCAATAACT 301 AGCATAACCC CTTGGGGCCT CTAAACGGGT CTTGAGGGGT TTTTTGCATG GTCATAGCTG 361 TTTCCTGAGA GCTTGGCAGG TGATGACACA CATTAACAAA TTTCGTGAGG AGTCTCCAGA 421 AGAATGCCAT TAATTTCCAT AGGCTCCGCC CCCCTGACGA GCATCACAAA AATCGACGCT 481 CAAGTCAGAG GTGGCGAAAC CCGACAGGAC TATAAAGATA CCAGGCGTTT CCCCCTGGAA 541 GCTCCCTCGT GCGCTCTCCT GTTCCGACCC TGCCGCTTAC CGGATACCTG TCCGCCTTTC 601 TCCCTTCGGG AAGCGTGGCG CTTTCTCATA GCTCACGCTG TAGGTATCTC AGTTCGGTGT 661 AGGTCGTTCG CTCCAAGCTG GGCTGTGTGC ACGAACCCCC CGTTCAGCCC GACCGCTGCG 721 CCTTATCCGG TAACTATCGT CTTGAGTCCA ACCCGGTAAG ACACGACTTA TCGCCACTGG 781 CAGCAGCCAC TGGTAACAGG ATTAGCAGAG CGAGGTATGT AGGCGGTGCT ACAGAGTTCT 841 TGAAGTGGTG GCCTAACTAC GGCTACACTA GAAGAACAGT ATTTGGTATC TGCGCTCTGC 901 TGAAGCCAGT TACCTTCGGA AAAAGAGTTG GTAGCTCTTG ATCCGGCAAA CAAACCACCG 961 CTGGTAGCGG TGGTTTTTTT GTTTGCAAGC AGCAGATTAC GCGCAGAAAA AAAGGATCTC 1021 AAGAAGGCCT ACTATTAGCA ACAACGATCC TTTGATCTTT TCTACGGGGT CTGACGCTCA 1081 GTGGAACGAA AACTCACGTT AAGGGATTTT GGTCATGAGA TTATCAAAAA GGATCTTCAC 1141 CTAGATCCTT TTAAATTAAA AATGAAGTTT TAAATCAATC TAAAGTATAT ATGAGTAAAC 1201 TTGGTCTGAC AGTTACCAAT GCTTAATCAG TGAGGCACCT ATCTCAGCGA TCTGTCTATT 1261 TCGTTCATCC ATAGTTGCCT GACTCCCCGT CGTGTAGATA ACTACGATAC GGGAGGGCTT 1321 ACCATCTGGC CCCAGTGCTG CAATGATACC GCGAGAACCA CGCTCACCGG CTCCAGATTT 1381 ATCAGCAATA AACCAGCCAG CCGGAAGGGC CGAGCGCAGA AGTGGTCCTG CAACTTTATC 1441 CGCCTCCATC CAGTCTATTA ATTGTTGCCG GGAAGCTAGA GTAAGTAGTT CGCCAGTTAA 1501 TAGTTTGCGC AACGTTGTTG CCATTGCTAC AGGCATCGTG GTGTCACGCT CGTCGTTTGG 1561 TATGGCTTCA TTCAGCTCCG GTTCCCAACG ATCAAGGCGA GTTACATGAT CCCCCATGTT 1621 GTGCAAAAAA GCGGTTAGCT CCTTCGGTCC TCCGATCGTT GTCAGAAGTA AGTTGGCCGC 1681 AGTGTTATCA CTCATGGTTA TGGCAGCACT GCATAATTCT CTTACTGTCA TGCCATCCGT 1741 AAGATGCTTT TCTGTGACTG GTGAGTACTC AACCAAGTCA TTCTGAGAAT AGTGTATGCG 1801 GCGACCGAGT TGCTCTTGCC CGGCGTCAAT ACGGGATAAT ACCGCGCCAC ATAGCAGAAC 1861 TTTAAAAGTG CTCATCATTG GAAAACGTTC TTCGGGGCGA AAACTCTCAA GGATCTTACC 1921 GCTGTTGAGA TCCAGTTCGA TGTAACCCAC TCGTGCACCC AACTGATCTT CAGCATCTTT 1981 TACTTTCACC AGCGTTTCTG GGTGAGCAAA AACAGGAAGG CAAAATGCCG CAAAAAAGGG 2041 AATAAGGGCG ACACGGAAAT GTTGAATACT CATACTCTTC CTTTTTCAAT ATTATTGAAG 2101 CATTTATCAG GGTTATTGTC TCATGAGCGG ATACATATTT GAATGTATTT AGAAAAATAA 2161 ACAAATAGGG GTTCCGCGCA CCTGCACCAG TCAGTAAAAC GACGGCCAGT AGTCAAAAGC 2221 CTCCGACCGG AGGCTTTTGA CTTGGTTCAG GTGGAGTGGG AGAACGATGA TGCTCACTCT 2281 CGGGCTCCTT CATCTGACAA TATGCAACCC CTATCACCAT CGATTATTTC TACGAACGGT 2341 GTTGTTCTCA TAGTTTGGGC ATATTTCCCT TGTAGGTGTG AAATCACTTA GCTTCGCGCC 2401 GTAGTCTTAA AGGGTCTCA //