LOCUS BBF10K_003418 2410 bp DNA circular 17-Jun-2025 ACCESSION BBF10K_003418 VERSION BBF10K_003418.1 FEATURES Location/Qualifiers CDS 5..30 /label=T2C_TEVcut CDS complement(1203..2062) /label=AmpR promoter complement(2064..2167) /label=AmpR promoter primer_bind 2186..2202 /label=M13 fwd primer_bind complement(341..356) /label=M13 rev rep_origin complement(424..1011) /label=ColE1 primer_bind 258..276 /label=SLiCE-for primer_bind complement(2230..2247) /label=SLiCE-rev misc_feature 1015..1204 /label=spacer CDS 375..425 /label=spacer terminator 291..338 /label=T7 terminator terminator 2203..2234 /label=tonB terminator misc_feature 253..2253 /label=pOpen_v3 backbone misc_feature 2404..2409 /label=BsaI_site misc_feature complement(36..41) /label=BsaI_site misc_feature 1..4 /label=GTCA misc_feature 31..34 /label=AATG ORIGIN 1 GTCAATGGAA AATCTTTATT TTCAAGGTGG AATGCGAGAC CGAAAACCTA TGGACTTTGT 61 TTCGGGTAGC ACCAGAAATC TAAACCATGT GAATGTGAAC GTGGCGTGCG TACACCTTAA 121 TCTCCGGTTC ATGCTAAGGA TGTGGCTGCA TGCTATGTTG ATACACCTAC AAGCGCGGGT 181 GATATTACAA CTAGGCTCTA TAAGGCGTTA GCCTAGGGAT AACAGGGTAA TTATCTTCAA 241 ATCCGCGGTG AGCGCTGAGG TGTCAATCGT CGGAGCCGCT GAGCAATAAC TAGCATAACC 301 CCTTGGGGCC TCTAAACGGG TCTTGAGGGG TTTTTTGCAT GGTCATAGCT GTTTCCTGAG 361 AGCTTGGCAG GTGATGACAC ACATTAACAA ATTTCGTGAG GAGTCTCCAG AAGAATGCCA 421 TTAATTTCCA TAGGCTCCGC CCCCCTGACG AGCATCACAA AAATCGACGC TCAAGTCAGA 481 GGTGGCGAAA CCCGACAGGA CTATAAAGAT ACCAGGCGTT TCCCCCTGGA AGCTCCCTCG 541 TGCGCTCTCC TGTTCCGACC CTGCCGCTTA CCGGATACCT GTCCGCCTTT CTCCCTTCGG 601 GAAGCGTGGC GCTTTCTCAT AGCTCACGCT GTAGGTATCT CAGTTCGGTG TAGGTCGTTC 661 GCTCCAAGCT GGGCTGTGTG CACGAACCCC CCGTTCAGCC CGACCGCTGC GCCTTATCCG 721 GTAACTATCG TCTTGAGTCC AACCCGGTAA GACACGACTT ATCGCCACTG GCAGCAGCCA 781 CTGGTAACAG GATTAGCAGA GCGAGGTATG TAGGCGGTGC TACAGAGTTC TTGAAGTGGT 841 GGCCTAACTA CGGCTACACT AGAAGAACAG TATTTGGTAT CTGCGCTCTG CTGAAGCCAG 901 TTACCTTCGG AAAAAGAGTT GGTAGCTCTT GATCCGGCAA ACAAACCACC GCTGGTAGCG 961 GTGGTTTTTT TGTTTGCAAG CAGCAGATTA CGCGCAGAAA AAAAGGATCT CAAGAAGGCC 1021 TACTATTAGC AACAACGATC CTTTGATCTT TTCTACGGGG TCTGACGCTC AGTGGAACGA 1081 AAACTCACGT TAAGGGATTT TGGTCATGAG ATTATCAAAA AGGATCTTCA CCTAGATCCT 1141 TTTAAATTAA AAATGAAGTT TTAAATCAAT CTAAAGTATA TATGAGTAAA CTTGGTCTGA 1201 CAGTTACCAA TGCTTAATCA GTGAGGCACC TATCTCAGCG ATCTGTCTAT TTCGTTCATC 1261 CATAGTTGCC TGACTCCCCG TCGTGTAGAT AACTACGATA CGGGAGGGCT TACCATCTGG 1321 CCCCAGTGCT GCAATGATAC CGCGAGAACC ACGCTCACCG GCTCCAGATT TATCAGCAAT 1381 AAACCAGCCA GCCGGAAGGG CCGAGCGCAG AAGTGGTCCT GCAACTTTAT CCGCCTCCAT 1441 CCAGTCTATT AATTGTTGCC GGGAAGCTAG AGTAAGTAGT TCGCCAGTTA ATAGTTTGCG 1501 CAACGTTGTT GCCATTGCTA CAGGCATCGT GGTGTCACGC TCGTCGTTTG GTATGGCTTC 1561 ATTCAGCTCC GGTTCCCAAC GATCAAGGCG AGTTACATGA TCCCCCATGT TGTGCAAAAA 1621 AGCGGTTAGC TCCTTCGGTC CTCCGATCGT TGTCAGAAGT AAGTTGGCCG CAGTGTTATC 1681 ACTCATGGTT ATGGCAGCAC TGCATAATTC TCTTACTGTC ATGCCATCCG TAAGATGCTT 1741 TTCTGTGACT GGTGAGTACT CAACCAAGTC ATTCTGAGAA TAGTGTATGC GGCGACCGAG 1801 TTGCTCTTGC CCGGCGTCAA TACGGGATAA TACCGCGCCA CATAGCAGAA CTTTAAAAGT 1861 GCTCATCATT GGAAAACGTT CTTCGGGGCG AAAACTCTCA AGGATCTTAC CGCTGTTGAG 1921 ATCCAGTTCG ATGTAACCCA CTCGTGCACC CAACTGATCT TCAGCATCTT TTACTTTCAC 1981 CAGCGTTTCT GGGTGAGCAA AAACAGGAAG GCAAAATGCC GCAAAAAAGG GAATAAGGGC 2041 GACACGGAAA TGTTGAATAC TCATACTCTT CCTTTTTCAA TATTATTGAA GCATTTATCA 2101 GGGTTATTGT CTCATGAGCG GATACATATT TGAATGTATT TAGAAAAATA AACAAATAGG 2161 GGTTCCGCGC ACCTGCACCA GTCAGTAAAA CGACGGCCAG TAGTCAAAAG CCTCCGACCG 2221 GAGGCTTTTG ACTTGGTTCA GGTGGAGTGG GAGAACGATG ATGCTCACTC TCGTCGATGC 2281 ACACTCCCAT CTTTGAAAAC GCATAAACAT ACAAGTGGAC AGATAATGGG TACGGGCTTC 2341 TAATACATCC AACACTCTAC GTCCTCTTCA AGAGCTAGAA GAGCACCCTG CAGTTGGAAA 2401 AGAGGTCTCA //