LOCUS       BBF10K_003419           2434 bp    DNA     circular     29-Apr-2025
ACCESSION   BBF10K_003419
VERSION     BBF10K_003419.1  
FEATURES             Location/Qualifiers
     misc_feature    5..154
                     /label=BC_BCD2_His_TEVcut
     CDS             complement(1277..2136)
                     /label=AmpR
     promoter        complement(2138..2241)
                     /label=AmpR promoter
     primer_bind     2260..2276
                     /label=M13 fwd
     primer_bind     complement(415..430)
                     /label=M13 rev
     rep_origin      complement(498..1085)
                     /label=ColE1
     primer_bind     332..350
                     /label=SLiCE-for
     primer_bind     complement(2304..2321)
                     /label=SLiCE-rev
     misc_feature    1089..1278
                     /label=spacer
     CDS             449..499
                     /label=spacer
     terminator      365..412
                     /label=T7 terminator
     terminator      2277..2308
                     /label=tonB terminator
     misc_feature    327..2327
                     /label=pOpen_v3 backbone
     misc_feature    2428..2433
                     /label=BsaI_site
     misc_feature    complement(160..165)
                     /label=BsaI_site
     misc_feature    1..4
                     /label=TACT
     misc_feature    155..158
                     /label=AATG
ORIGIN
        1 TACTGGGCCC AAGTTCACTT AAAAAGGAGA TCAACAATGA AAGCAATTTT CGTACTGAAA
       61 CATCTTAATC ATGCTAAGGA GGTTTTCTAA TGGGCAGCAG CCATCACCAT CACCATCACA
      121 GCAGCGGCGG AGAAAATCTT TATTTTCAAG GTGGAATGCG AGACCAAGTC TTAGAACTGG
      181 ACGTGCCGTT TCTCTGCGAA TAATACCTCG AGCTGTACCG TTGTTGCGCT GCCTAGATGC
      241 AGTGCAGTCT CATGCTAGCC ACGTATTTTA CTCGCATTGC AATTGAATAG GGATAACAGG
      301 GTAATCCCCA CCATCCTGGG GGTAGGCGCT GAGGTGTCAA TCGTCGGAGC CGCTGAGCAA
      361 TAACTAGCAT AACCCCTTGG GGCCTCTAAA CGGGTCTTGA GGGGTTTTTT GCATGGTCAT
      421 AGCTGTTTCC TGAGAGCTTG GCAGGTGATG ACACACATTA ACAAATTTCG TGAGGAGTCT
      481 CCAGAAGAAT GCCATTAATT TCCATAGGCT CCGCCCCCCT GACGAGCATC ACAAAAATCG
      541 ACGCTCAAGT CAGAGGTGGC GAAACCCGAC AGGACTATAA AGATACCAGG CGTTTCCCCC
      601 TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC GACCCTGCCG CTTACCGGAT ACCTGTCCGC
      661 CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC TCATAGCTCA CGCTGTAGGT ATCTCAGTTC
      721 GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG TGTGCACGAA CCCCCCGTTC AGCCCGACCG
      781 CTGCGCCTTA TCCGGTAACT ATCGTCTTGA GTCCAACCCG GTAAGACACG ACTTATCGCC
      841 ACTGGCAGCA GCCACTGGTA ACAGGATTAG CAGAGCGAGG TATGTAGGCG GTGCTACAGA
      901 GTTCTTGAAG TGGTGGCCTA ACTACGGCTA CACTAGAAGA ACAGTATTTG GTATCTGCGC
      961 TCTGCTGAAG CCAGTTACCT TCGGAAAAAG AGTTGGTAGC TCTTGATCCG GCAAACAAAC
     1021 CACCGCTGGT AGCGGTGGTT TTTTTGTTTG CAAGCAGCAG ATTACGCGCA GAAAAAAAGG
     1081 ATCTCAAGAA GGCCTACTAT TAGCAACAAC GATCCTTTGA TCTTTTCTAC GGGGTCTGAC
     1141 GCTCAGTGGA ACGAAAACTC ACGTTAAGGG ATTTTGGTCA TGAGATTATC AAAAAGGATC
     1201 TTCACCTAGA TCCTTTTAAA TTAAAAATGA AGTTTTAAAT CAATCTAAAG TATATATGAG
     1261 TAAACTTGGT CTGACAGTTA CCAATGCTTA ATCAGTGAGG CACCTATCTC AGCGATCTGT
     1321 CTATTTCGTT CATCCATAGT TGCCTGACTC CCCGTCGTGT AGATAACTAC GATACGGGAG
     1381 GGCTTACCAT CTGGCCCCAG TGCTGCAATG ATACCGCGAG AACCACGCTC ACCGGCTCCA
     1441 GATTTATCAG CAATAAACCA GCCAGCCGGA AGGGCCGAGC GCAGAAGTGG TCCTGCAACT
     1501 TTATCCGCCT CCATCCAGTC TATTAATTGT TGCCGGGAAG CTAGAGTAAG TAGTTCGCCA
     1561 GTTAATAGTT TGCGCAACGT TGTTGCCATT GCTACAGGCA TCGTGGTGTC ACGCTCGTCG
     1621 TTTGGTATGG CTTCATTCAG CTCCGGTTCC CAACGATCAA GGCGAGTTAC ATGATCCCCC
     1681 ATGTTGTGCA AAAAAGCGGT TAGCTCCTTC GGTCCTCCGA TCGTTGTCAG AAGTAAGTTG
     1741 GCCGCAGTGT TATCACTCAT GGTTATGGCA GCACTGCATA ATTCTCTTAC TGTCATGCCA
     1801 TCCGTAAGAT GCTTTTCTGT GACTGGTGAG TACTCAACCA AGTCATTCTG AGAATAGTGT
     1861 ATGCGGCGAC CGAGTTGCTC TTGCCCGGCG TCAATACGGG ATAATACCGC GCCACATAGC
     1921 AGAACTTTAA AAGTGCTCAT CATTGGAAAA CGTTCTTCGG GGCGAAAACT CTCAAGGATC
     1981 TTACCGCTGT TGAGATCCAG TTCGATGTAA CCCACTCGTG CACCCAACTG ATCTTCAGCA
     2041 TCTTTTACTT TCACCAGCGT TTCTGGGTGA GCAAAAACAG GAAGGCAAAA TGCCGCAAAA
     2101 AAGGGAATAA GGGCGACACG GAAATGTTGA ATACTCATAC TCTTCCTTTT TCAATATTAT
     2161 TGAAGCATTT ATCAGGGTTA TTGTCTCATG AGCGGATACA TATTTGAATG TATTTAGAAA
     2221 AATAAACAAA TAGGGGTTCC GCGCACCTGC ACCAGTCAGT AAAACGACGG CCAGTAGTCA
     2281 AAAGCCTCCG ACCGGAGGCT TTTGACTTGG TTCAGGTGGA GTGGGAGAAC GATGATGCTC
     2341 ACTCTCGTGC TCTTATCACA TTTGCTTCGA CGACTGCCGC CTTCGCTGTT TCCCTAGACA
     2401 CTCAACAATA AGCGCTTTTT GTAGGCAGGT CTCA
//