LOCUS       BBF10K_003420           2997 bp    DNA     circular     29-Apr-2025
ACCESSION   BBF10K_003420
VERSION     BBF10K_003420.1  
FEATURES             Location/Qualifiers
     misc_feature    5..877
                     /label=BC_BCD2_His_fuGFP_TEVcut
     CDS             complement(1920..2779)
                     /label=AmpR
     promoter        complement(2781..2884)
                     /label=AmpR promoter
     primer_bind     2903..2919
                     /label=M13 fwd
     primer_bind     complement(1058..1073)
                     /label=M13 rev
     rep_origin      complement(1141..1728)
                     /label=ColE1
     primer_bind     975..993
                     /label=SLiCE-for
     primer_bind     complement(2947..2964)
                     /label=SLiCE-rev
     misc_feature    1732..1921
                     /label=spacer
     CDS             1092..1142
                     /label=spacer
     terminator      1008..1055
                     /label=T7 terminator
     terminator      2920..2951
                     /label=tonB terminator
     misc_feature    970..2970
                     /label=pOpen_v3 backbone
     misc_feature    2991..2996
                     /label=BsaI_site
     misc_feature    complement(883..888)
                     /label=BsaI_site
     misc_feature    1..4
                     /label=TACT
     misc_feature    878..881
                     /label=AATG
ORIGIN
        1 TACTGGGCCC AAGTTCACTT AAAAAGGAGA TCAACAATGA AAGCAATTTT CGTACTGAAA
       61 CATCTTAATC ATGCTAAGGA GGTTTTCTAA TGGGCAGCAG CCATCACCAT CACCATCACA
      121 GCAGCGGCGT TTCTAGCGGT GAAGATATCT TCTCAGGCCT GGTCCCGATT CTGATCGAGC
      181 TGGAGGGCGA TGTGAATGGC CACCGCTTCT CTGTTCGTGG TGAAGGTTAT GGCGACGCCT
      241 CTAACGGCAA ACTGGAAATT AAGTTCATCT GCACCACCGG TCGTCTGCCG GTTCCGTGGC
      301 CGACGCTGGT GACTACTCTG TCTTACGGTG TCCAGTGCTT CGCAAAATAC CCGGAACACA
      361 TGCGTCAGAA TGATTTTTTC AAGTCCGCCA TGCCAGATGG CTATGTTCAG GAACGTACCA
      421 TCAGCTTCAA AGAGGACGGT ACGTACAAAA CCCGTGCGGA AGTTAAATTT GAAGGTGAAG
      481 CGCTGGTGAA CCGTATCGAC TTAAAAGGTC TGGAGTTCAA GGAAGATGGC AACATTCTGG
      541 GCCACAAACT GGAATACAGC TTCAACAGCC ACTATGTTTA TATTACCGCG GACAAAAATC
      601 GCAACGGCCT GGAGGCGCAG TTCCGTATCC GTCACAACGT CGACGACGGT TCTGTCCAGC
      661 TTGCGGACCA TTACCAGCAG AACACCCCGA TCGGTGAGGG CCCAGTACTG CTCCCGGAGC
      721 AACACTACCT GACGACTAAC TCTGTCCTGT CCAAAGACCC GCAGGAACGC CGTGATCACA
      781 TGGTTCTGGT TGAATTTGTG ACCGCTGCCG GCCTGTCGCT GGGCATGGAC GAACTGTATA
      841 AATCCAGCGG CGGAGAAAAT CTTTATTTTC AAGGTGGAAT GCGAGACCAC CGCGTGGTTC
      901 TTACAGTATG CTGCGCCACA CGCTACTGGT TAGGGATAAC AGGGTAATCC CCGAGCATTT
      961 GCGCGTGTAC GCTGAGGTGT CAATCGTCGG AGCCGCTGAG CAATAACTAG CATAACCCCT
     1021 TGGGGCCTCT AAACGGGTCT TGAGGGGTTT TTTGCATGGT CATAGCTGTT TCCTGAGAGC
     1081 TTGGCAGGTG ATGACACACA TTAACAAATT TCGTGAGGAG TCTCCAGAAG AATGCCATTA
     1141 ATTTCCATAG GCTCCGCCCC CCTGACGAGC ATCACAAAAA TCGACGCTCA AGTCAGAGGT
     1201 GGCGAAACCC GACAGGACTA TAAAGATACC AGGCGTTTCC CCCTGGAAGC TCCCTCGTGC
     1261 GCTCTCCTGT TCCGACCCTG CCGCTTACCG GATACCTGTC CGCCTTTCTC CCTTCGGGAA
     1321 GCGTGGCGCT TTCTCATAGC TCACGCTGTA GGTATCTCAG TTCGGTGTAG GTCGTTCGCT
     1381 CCAAGCTGGG CTGTGTGCAC GAACCCCCCG TTCAGCCCGA CCGCTGCGCC TTATCCGGTA
     1441 ACTATCGTCT TGAGTCCAAC CCGGTAAGAC ACGACTTATC GCCACTGGCA GCAGCCACTG
     1501 GTAACAGGAT TAGCAGAGCG AGGTATGTAG GCGGTGCTAC AGAGTTCTTG AAGTGGTGGC
     1561 CTAACTACGG CTACACTAGA AGAACAGTAT TTGGTATCTG CGCTCTGCTG AAGCCAGTTA
     1621 CCTTCGGAAA AAGAGTTGGT AGCTCTTGAT CCGGCAAACA AACCACCGCT GGTAGCGGTG
     1681 GTTTTTTTGT TTGCAAGCAG CAGATTACGC GCAGAAAAAA AGGATCTCAA GAAGGCCTAC
     1741 TATTAGCAAC AACGATCCTT TGATCTTTTC TACGGGGTCT GACGCTCAGT GGAACGAAAA
     1801 CTCACGTTAA GGGATTTTGG TCATGAGATT ATCAAAAAGG ATCTTCACCT AGATCCTTTT
     1861 AAATTAAAAA TGAAGTTTTA AATCAATCTA AAGTATATAT GAGTAAACTT GGTCTGACAG
     1921 TTACCAATGC TTAATCAGTG AGGCACCTAT CTCAGCGATC TGTCTATTTC GTTCATCCAT
     1981 AGTTGCCTGA CTCCCCGTCG TGTAGATAAC TACGATACGG GAGGGCTTAC CATCTGGCCC
     2041 CAGTGCTGCA ATGATACCGC GAGAACCACG CTCACCGGCT CCAGATTTAT CAGCAATAAA
     2101 CCAGCCAGCC GGAAGGGCCG AGCGCAGAAG TGGTCCTGCA ACTTTATCCG CCTCCATCCA
     2161 GTCTATTAAT TGTTGCCGGG AAGCTAGAGT AAGTAGTTCG CCAGTTAATA GTTTGCGCAA
     2221 CGTTGTTGCC ATTGCTACAG GCATCGTGGT GTCACGCTCG TCGTTTGGTA TGGCTTCATT
     2281 CAGCTCCGGT TCCCAACGAT CAAGGCGAGT TACATGATCC CCCATGTTGT GCAAAAAAGC
     2341 GGTTAGCTCC TTCGGTCCTC CGATCGTTGT CAGAAGTAAG TTGGCCGCAG TGTTATCACT
     2401 CATGGTTATG GCAGCACTGC ATAATTCTCT TACTGTCATG CCATCCGTAA GATGCTTTTC
     2461 TGTGACTGGT GAGTACTCAA CCAAGTCATT CTGAGAATAG TGTATGCGGC GACCGAGTTG
     2521 CTCTTGCCCG GCGTCAATAC GGGATAATAC CGCGCCACAT AGCAGAACTT TAAAAGTGCT
     2581 CATCATTGGA AAACGTTCTT CGGGGCGAAA ACTCTCAAGG ATCTTACCGC TGTTGAGATC
     2641 CAGTTCGATG TAACCCACTC GTGCACCCAA CTGATCTTCA GCATCTTTTA CTTTCACCAG
     2701 CGTTTCTGGG TGAGCAAAAA CAGGAAGGCA AAATGCCGCA AAAAAGGGAA TAAGGGCGAC
     2761 ACGGAAATGT TGAATACTCA TACTCTTCCT TTTTCAATAT TATTGAAGCA TTTATCAGGG
     2821 TTATTGTCTC ATGAGCGGAT ACATATTTGA ATGTATTTAG AAAAATAAAC AAATAGGGGT
     2881 TCCGCGCACC TGCACCAGTC AGTAAAACGA CGGCCAGTAG TCAAAAGCCT CCGACCGGAG
     2941 GCTTTTGACT TGGTTCAGGT GGAGTGGGAG AACGATGATG CTCACTCTCG GGTCTCA
//