LOCUS       BBF10K_003421           2640 bp    DNA     circular     29-Apr-2025
ACCESSION   BBF10K_003421
VERSION     BBF10K_003421.1  
FEATURES             Location/Qualifiers
     CDS             5..520
                     /label=T2T3_int_∆I
     CDS             complement(1563..2422)
                     /label=AmpR
     promoter        complement(2424..2527)
                     /label=AmpR promoter
     primer_bind     2546..2562
                     /label=M13 fwd
     primer_bind     complement(701..716)
                     /label=M13 rev
     rep_origin      complement(784..1371)
                     /label=ColE1
     primer_bind     618..636
                     /label=SLiCE-for
     primer_bind     complement(2590..2607)
                     /label=SLiCE-rev
     misc_feature    1375..1564
                     /label=spacer
     CDS             735..785
                     /label=spacer
     terminator      651..698
                     /label=T7 terminator
     terminator      2563..2594
                     /label=tonB terminator
     misc_feature    613..2613
                     /label=pOpen_v3 backbone
     misc_feature    2634..2639
                     /label=BsaI_site
     misc_feature    complement(526..531)
                     /label=BsaI_site
     misc_feature    1..4
                     /label=GTCA
     misc_feature    521..524
                     /label=TCCA
ORIGIN
        1 GTCAATCTCA GAATTCGCAT TGGCCGAGGG AACTCGTATC TTCGATCCTG TAACTGGTAC
       61 TACTCATCGC ATTGAAGATG TTGTGGGAGG GCGTAAACCT ATCCATGTAG TCGCAGCTGC
      121 GAAGGACGGC ACCCTGCACG CACGCCCTGT GGTATCCTGG TTTGATCAGG GCACTCGCGA
      181 TGTCATCGGT CTGCGCATTG CGGGAGGTGC AATTCTGTGG GCTACTCCGG ACCACAAGGT
      241 CCTGACAGAG TATGGGTGGC GTGCCGCTGG GGAGCTGCGC AAAGGAGATC GTGTGGCCCA
      301 GCCACGTCGT TTTGACGGAT TTGGCGATAG TGCCCCAATC CCAGCACGTG TCCAGGCGCT
      361 GGCTGATGCT CTGGATGATA AGTTCTTACA TGACATGCTT GCCGAAGAAC TGCGCTATTC
      421 TGTGATTCGT GAGGTGCTTC CTACTCGTCG TGCTCGCACG TTCGGACTGG AAGTTGAAGA
      481 ACTGCATACA CTTGTCGCCG AGGGTGTCGT CGTGCACAAC TCCACGAGAC CACGACTGCC
      541 GACATGATCT ATTCGGTTCA CTATTACGCC GTATAGGGAT AACAGGGTAA TGTCGTTATA
      601 CAGTGCATCC TTCGCTGAGG TGTCAATCGT CGGAGCCGCT GAGCAATAAC TAGCATAACC
      661 CCTTGGGGCC TCTAAACGGG TCTTGAGGGG TTTTTTGCAT GGTCATAGCT GTTTCCTGAG
      721 AGCTTGGCAG GTGATGACAC ACATTAACAA ATTTCGTGAG GAGTCTCCAG AAGAATGCCA
      781 TTAATTTCCA TAGGCTCCGC CCCCCTGACG AGCATCACAA AAATCGACGC TCAAGTCAGA
      841 GGTGGCGAAA CCCGACAGGA CTATAAAGAT ACCAGGCGTT TCCCCCTGGA AGCTCCCTCG
      901 TGCGCTCTCC TGTTCCGACC CTGCCGCTTA CCGGATACCT GTCCGCCTTT CTCCCTTCGG
      961 GAAGCGTGGC GCTTTCTCAT AGCTCACGCT GTAGGTATCT CAGTTCGGTG TAGGTCGTTC
     1021 GCTCCAAGCT GGGCTGTGTG CACGAACCCC CCGTTCAGCC CGACCGCTGC GCCTTATCCG
     1081 GTAACTATCG TCTTGAGTCC AACCCGGTAA GACACGACTT ATCGCCACTG GCAGCAGCCA
     1141 CTGGTAACAG GATTAGCAGA GCGAGGTATG TAGGCGGTGC TACAGAGTTC TTGAAGTGGT
     1201 GGCCTAACTA CGGCTACACT AGAAGAACAG TATTTGGTAT CTGCGCTCTG CTGAAGCCAG
     1261 TTACCTTCGG AAAAAGAGTT GGTAGCTCTT GATCCGGCAA ACAAACCACC GCTGGTAGCG
     1321 GTGGTTTTTT TGTTTGCAAG CAGCAGATTA CGCGCAGAAA AAAAGGATCT CAAGAAGGCC
     1381 TACTATTAGC AACAACGATC CTTTGATCTT TTCTACGGGG TCTGACGCTC AGTGGAACGA
     1441 AAACTCACGT TAAGGGATTT TGGTCATGAG ATTATCAAAA AGGATCTTCA CCTAGATCCT
     1501 TTTAAATTAA AAATGAAGTT TTAAATCAAT CTAAAGTATA TATGAGTAAA CTTGGTCTGA
     1561 CAGTTACCAA TGCTTAATCA GTGAGGCACC TATCTCAGCG ATCTGTCTAT TTCGTTCATC
     1621 CATAGTTGCC TGACTCCCCG TCGTGTAGAT AACTACGATA CGGGAGGGCT TACCATCTGG
     1681 CCCCAGTGCT GCAATGATAC CGCGAGAACC ACGCTCACCG GCTCCAGATT TATCAGCAAT
     1741 AAACCAGCCA GCCGGAAGGG CCGAGCGCAG AAGTGGTCCT GCAACTTTAT CCGCCTCCAT
     1801 CCAGTCTATT AATTGTTGCC GGGAAGCTAG AGTAAGTAGT TCGCCAGTTA ATAGTTTGCG
     1861 CAACGTTGTT GCCATTGCTA CAGGCATCGT GGTGTCACGC TCGTCGTTTG GTATGGCTTC
     1921 ATTCAGCTCC GGTTCCCAAC GATCAAGGCG AGTTACATGA TCCCCCATGT TGTGCAAAAA
     1981 AGCGGTTAGC TCCTTCGGTC CTCCGATCGT TGTCAGAAGT AAGTTGGCCG CAGTGTTATC
     2041 ACTCATGGTT ATGGCAGCAC TGCATAATTC TCTTACTGTC ATGCCATCCG TAAGATGCTT
     2101 TTCTGTGACT GGTGAGTACT CAACCAAGTC ATTCTGAGAA TAGTGTATGC GGCGACCGAG
     2161 TTGCTCTTGC CCGGCGTCAA TACGGGATAA TACCGCGCCA CATAGCAGAA CTTTAAAAGT
     2221 GCTCATCATT GGAAAACGTT CTTCGGGGCG AAAACTCTCA AGGATCTTAC CGCTGTTGAG
     2281 ATCCAGTTCG ATGTAACCCA CTCGTGCACC CAACTGATCT TCAGCATCTT TTACTTTCAC
     2341 CAGCGTTTCT GGGTGAGCAA AAACAGGAAG GCAAAATGCC GCAAAAAAGG GAATAAGGGC
     2401 GACACGGAAA TGTTGAATAC TCATACTCTT CCTTTTTCAA TATTATTGAA GCATTTATCA
     2461 GGGTTATTGT CTCATGAGCG GATACATATT TGAATGTATT TAGAAAAATA AACAAATAGG
     2521 GGTTCCGCGC ACCTGCACCA GTCAGTAAAA CGACGGCCAG TAGTCAAAAG CCTCCGACCG
     2581 GAGGCTTTTG ACTTGGTTCA GGTGGAGTGG GAGAACGATG ATGCTCACTC TCGGGTCTCA
//