LOCUS       BBF10K_003422           2421 bp    DNA     circular     29-Apr-2025
ACCESSION   BBF10K_003422
VERSION     BBF10K_003422.1  
FEATURES             Location/Qualifiers
     CDS             5..301
                     /label=T2T3_SUMO
     CDS             complement(1344..2203)
                     /label=AmpR
     promoter        complement(2205..2308)
                     /label=AmpR promoter
     primer_bind     2327..2343
                     /label=M13 fwd
     primer_bind     complement(482..497)
                     /label=M13 rev
     rep_origin      complement(565..1152)
                     /label=ColE1
     primer_bind     399..417
                     /label=SLiCE-for
     primer_bind     complement(2371..2388)
                     /label=SLiCE-rev
     misc_feature    1156..1345
                     /label=spacer
     CDS             516..566
                     /label=spacer
     terminator      432..479
                     /label=T7 terminator
     terminator      2344..2375
                     /label=tonB terminator
     misc_feature    394..2394
                     /label=pOpen_v3 backbone
     misc_feature    2415..2420
                     /label=BsaI_site
     misc_feature    complement(307..312)
                     /label=BsaI_site
     misc_feature    1..4
                     /label=GTCA
     misc_feature    302..305
                     /label=TCCA
ORIGIN
        1 GTCAGATAGC GAAGTGAACC AGGAAGCGAA GCCAGAGGTG AAACCGGAGG TTAAACCAGA
       61 AACCCATATT AACCTGAAAG TGAGCGATGG CAGCAGCGAA ATTTTTTTTA AAATTAAAAA
      121 AACCACCCCG CTGCGCCGCC TGATGGAAGC GTTTGCGAAA CGCCAGGGCA AAGAAATGGA
      181 TAGCCTGCGC TTTCTGTATG ATGGCATTCG CATTCAGGCG GATCAGACCC CGGAGGACCT
      241 GGACATGGAA GATAACGATA TTATTGAAGC GCATCGCGAA CAGATTGGCG GCGCGACCTA
      301 TTCCACGAGA CCACGCATGT AGGCACCATC TATGAGTATC ACAACCACTT TAGGTAGGGA
      361 TAACAGGGTA ATGGACCTAC TTCGTACTTA GACCGCTGAG GTGTCAATCG TCGGAGCCGC
      421 TGAGCAATAA CTAGCATAAC CCCTTGGGGC CTCTAAACGG GTCTTGAGGG GTTTTTTGCA
      481 TGGTCATAGC TGTTTCCTGA GAGCTTGGCA GGTGATGACA CACATTAACA AATTTCGTGA
      541 GGAGTCTCCA GAAGAATGCC ATTAATTTCC ATAGGCTCCG CCCCCCTGAC GAGCATCACA
      601 AAAATCGACG CTCAAGTCAG AGGTGGCGAA ACCCGACAGG ACTATAAAGA TACCAGGCGT
      661 TTCCCCCTGG AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC CCTGCCGCTT ACCGGATACC
      721 TGTCCGCCTT TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA TAGCTCACGC TGTAGGTATC
      781 TCAGTTCGGT GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT GCACGAACCC CCCGTTCAGC
      841 CCGACCGCTG CGCCTTATCC GGTAACTATC GTCTTGAGTC CAACCCGGTA AGACACGACT
      901 TATCGCCACT GGCAGCAGCC ACTGGTAACA GGATTAGCAG AGCGAGGTAT GTAGGCGGTG
      961 CTACAGAGTT CTTGAAGTGG TGGCCTAACT ACGGCTACAC TAGAAGAACA GTATTTGGTA
     1021 TCTGCGCTCT GCTGAAGCCA GTTACCTTCG GAAAAAGAGT TGGTAGCTCT TGATCCGGCA
     1081 AACAAACCAC CGCTGGTAGC GGTGGTTTTT TTGTTTGCAA GCAGCAGATT ACGCGCAGAA
     1141 AAAAAGGATC TCAAGAAGGC CTACTATTAG CAACAACGAT CCTTTGATCT TTTCTACGGG
     1201 GTCTGACGCT CAGTGGAACG AAAACTCACG TTAAGGGATT TTGGTCATGA GATTATCAAA
     1261 AAGGATCTTC ACCTAGATCC TTTTAAATTA AAAATGAAGT TTTAAATCAA TCTAAAGTAT
     1321 ATATGAGTAA ACTTGGTCTG ACAGTTACCA ATGCTTAATC AGTGAGGCAC CTATCTCAGC
     1381 GATCTGTCTA TTTCGTTCAT CCATAGTTGC CTGACTCCCC GTCGTGTAGA TAACTACGAT
     1441 ACGGGAGGGC TTACCATCTG GCCCCAGTGC TGCAATGATA CCGCGAGAAC CACGCTCACC
     1501 GGCTCCAGAT TTATCAGCAA TAAACCAGCC AGCCGGAAGG GCCGAGCGCA GAAGTGGTCC
     1561 TGCAACTTTA TCCGCCTCCA TCCAGTCTAT TAATTGTTGC CGGGAAGCTA GAGTAAGTAG
     1621 TTCGCCAGTT AATAGTTTGC GCAACGTTGT TGCCATTGCT ACAGGCATCG TGGTGTCACG
     1681 CTCGTCGTTT GGTATGGCTT CATTCAGCTC CGGTTCCCAA CGATCAAGGC GAGTTACATG
     1741 ATCCCCCATG TTGTGCAAAA AAGCGGTTAG CTCCTTCGGT CCTCCGATCG TTGTCAGAAG
     1801 TAAGTTGGCC GCAGTGTTAT CACTCATGGT TATGGCAGCA CTGCATAATT CTCTTACTGT
     1861 CATGCCATCC GTAAGATGCT TTTCTGTGAC TGGTGAGTAC TCAACCAAGT CATTCTGAGA
     1921 ATAGTGTATG CGGCGACCGA GTTGCTCTTG CCCGGCGTCA ATACGGGATA ATACCGCGCC
     1981 ACATAGCAGA ACTTTAAAAG TGCTCATCAT TGGAAAACGT TCTTCGGGGC GAAAACTCTC
     2041 AAGGATCTTA CCGCTGTTGA GATCCAGTTC GATGTAACCC ACTCGTGCAC CCAACTGATC
     2101 TTCAGCATCT TTTACTTTCA CCAGCGTTTC TGGGTGAGCA AAAACAGGAA GGCAAAATGC
     2161 CGCAAAAAAG GGAATAAGGG CGACACGGAA ATGTTGAATA CTCATACTCT TCCTTTTTCA
     2221 ATATTATTGA AGCATTTATC AGGGTTATTG TCTCATGAGC GGATACATAT TTGAATGTAT
     2281 TTAGAAAAAT AAACAAATAG GGGTTCCGCG CACCTGCACC AGTCAGTAAA ACGACGGCCA
     2341 GTAGTCAAAA GCCTCCGACC GGAGGCTTTT GACTTGGTTC AGGTGGAGTG GGAGAACGAT
     2401 GATGCTCACT CTCGGGTCTC A
//