LOCUS       BBF10K_003423           2856 bp    DNA     circular     29-Apr-2025
ACCESSION   BBF10K_003423
VERSION     BBF10K_003423.1  
FEATURES             Location/Qualifiers
     CDS             5..736
                     /label=T2T3_fuGFP
     CDS             complement(1779..2638)
                     /label=AmpR
     promoter        complement(2640..2743)
                     /label=AmpR promoter
     primer_bind     2762..2778
                     /label=M13 fwd
     primer_bind     complement(917..932)
                     /label=M13 rev
     rep_origin      complement(1000..1587)
                     /label=ColE1
     primer_bind     834..852
                     /label=SLiCE-for
     primer_bind     complement(2806..2823)
                     /label=SLiCE-rev
     misc_feature    1591..1780
                     /label=spacer
     CDS             951..1001
                     /label=spacer
     terminator      867..914
                     /label=T7 terminator
     terminator      2779..2810
                     /label=tonB terminator
     misc_feature    829..2829
                     /label=pOpen_v3 backbone
     misc_feature    2850..2855
                     /label=BsaI_site
     misc_feature    complement(742..747)
                     /label=BsaI_site
     misc_feature    1..4
                     /label=GTCA
     misc_feature    737..740
                     /label=TCCA
ORIGIN
        1 GTCAGTTTCT AGCGGTGAAG ATATCTTCTC AGGCCTGGTC CCGATTCTGA TCGAGCTGGA
       61 GGGCGATGTG AATGGCCACC GCTTCTCTGT TCGTGGTGAA GGTTATGGCG ACGCCTCTAA
      121 CGGCAAACTG GAAATTAAGT TCATCTGCAC CACCGGTCGT CTGCCGGTTC CGTGGCCGAC
      181 GCTGGTGACT ACTCTGTCTT ACGGTGTCCA GTGCTTCGCA AAATACCCGG AACACATGCG
      241 TCAGAATGAT TTTTTCAAGT CCGCCATGCC AGATGGCTAT GTTCAGGAAC GTACCATCAG
      301 CTTCAAAGAG GACGGTACGT ACAAAACCCG TGCGGAAGTT AAATTTGAAG GTGAAGCGCT
      361 GGTGAACCGT ATCGACTTAA AAGGTCTGGA GTTCAAGGAA GATGGCAACA TTCTGGGCCA
      421 CAAACTGGAA TACAGCTTCA ACAGCCACTA TGTTTATATT ACCGCGGACA AAAATCGCAA
      481 CGGCCTGGAG GCGCAGTTCC GTATCCGTCA CAACGTCGAC GACGGTTCTG TCCAGCTTGC
      541 GGACCATTAC CAGCAGAACA CCCCGATCGG TGAGGGCCCA GTACTGCTCC CGGAGCAACA
      601 CTACCTGACG ACTAACTCTG TCCTGTCCAA AGACCCGCAG GAACGCCGTG ATCACATGGT
      661 TCTGGTTGAA TTTGTGACCG CTGCCGGCCT GTCGCTGGGC ATGGACGAAC TGTATAAATC
      721 CGGCGGTGGC GGTGGATCCA CGAGACCAGA CCCGTCCTTT GTTGTGATGT AAAAGTGACA
      781 TCGACACTCT AGGGATAACA GGGTAATAGT CCGCCAACCG CCACTGAGCG CTGAGGTGTC
      841 AATCGTCGGA GCCGCTGAGC AATAACTAGC ATAACCCCTT GGGGCCTCTA AACGGGTCTT
      901 GAGGGGTTTT TTGCATGGTC ATAGCTGTTT CCTGAGAGCT TGGCAGGTGA TGACACACAT
      961 TAACAAATTT CGTGAGGAGT CTCCAGAAGA ATGCCATTAA TTTCCATAGG CTCCGCCCCC
     1021 CTGACGAGCA TCACAAAAAT CGACGCTCAA GTCAGAGGTG GCGAAACCCG ACAGGACTAT
     1081 AAAGATACCA GGCGTTTCCC CCTGGAAGCT CCCTCGTGCG CTCTCCTGTT CCGACCCTGC
     1141 CGCTTACCGG ATACCTGTCC GCCTTTCTCC CTTCGGGAAG CGTGGCGCTT TCTCATAGCT
     1201 CACGCTGTAG GTATCTCAGT TCGGTGTAGG TCGTTCGCTC CAAGCTGGGC TGTGTGCACG
     1261 AACCCCCCGT TCAGCCCGAC CGCTGCGCCT TATCCGGTAA CTATCGTCTT GAGTCCAACC
     1321 CGGTAAGACA CGACTTATCG CCACTGGCAG CAGCCACTGG TAACAGGATT AGCAGAGCGA
     1381 GGTATGTAGG CGGTGCTACA GAGTTCTTGA AGTGGTGGCC TAACTACGGC TACACTAGAA
     1441 GAACAGTATT TGGTATCTGC GCTCTGCTGA AGCCAGTTAC CTTCGGAAAA AGAGTTGGTA
     1501 GCTCTTGATC CGGCAAACAA ACCACCGCTG GTAGCGGTGG TTTTTTTGTT TGCAAGCAGC
     1561 AGATTACGCG CAGAAAAAAA GGATCTCAAG AAGGCCTACT ATTAGCAACA ACGATCCTTT
     1621 GATCTTTTCT ACGGGGTCTG ACGCTCAGTG GAACGAAAAC TCACGTTAAG GGATTTTGGT
     1681 CATGAGATTA TCAAAAAGGA TCTTCACCTA GATCCTTTTA AATTAAAAAT GAAGTTTTAA
     1741 ATCAATCTAA AGTATATATG AGTAAACTTG GTCTGACAGT TACCAATGCT TAATCAGTGA
     1801 GGCACCTATC TCAGCGATCT GTCTATTTCG TTCATCCATA GTTGCCTGAC TCCCCGTCGT
     1861 GTAGATAACT ACGATACGGG AGGGCTTACC ATCTGGCCCC AGTGCTGCAA TGATACCGCG
     1921 AGAACCACGC TCACCGGCTC CAGATTTATC AGCAATAAAC CAGCCAGCCG GAAGGGCCGA
     1981 GCGCAGAAGT GGTCCTGCAA CTTTATCCGC CTCCATCCAG TCTATTAATT GTTGCCGGGA
     2041 AGCTAGAGTA AGTAGTTCGC CAGTTAATAG TTTGCGCAAC GTTGTTGCCA TTGCTACAGG
     2101 CATCGTGGTG TCACGCTCGT CGTTTGGTAT GGCTTCATTC AGCTCCGGTT CCCAACGATC
     2161 AAGGCGAGTT ACATGATCCC CCATGTTGTG CAAAAAAGCG GTTAGCTCCT TCGGTCCTCC
     2221 GATCGTTGTC AGAAGTAAGT TGGCCGCAGT GTTATCACTC ATGGTTATGG CAGCACTGCA
     2281 TAATTCTCTT ACTGTCATGC CATCCGTAAG ATGCTTTTCT GTGACTGGTG AGTACTCAAC
     2341 CAAGTCATTC TGAGAATAGT GTATGCGGCG ACCGAGTTGC TCTTGCCCGG CGTCAATACG
     2401 GGATAATACC GCGCCACATA GCAGAACTTT AAAAGTGCTC ATCATTGGAA AACGTTCTTC
     2461 GGGGCGAAAA CTCTCAAGGA TCTTACCGCT GTTGAGATCC AGTTCGATGT AACCCACTCG
     2521 TGCACCCAAC TGATCTTCAG CATCTTTTAC TTTCACCAGC GTTTCTGGGT GAGCAAAAAC
     2581 AGGAAGGCAA AATGCCGCAA AAAAGGGAAT AAGGGCGACA CGGAAATGTT GAATACTCAT
     2641 ACTCTTCCTT TTTCAATATT ATTGAAGCAT TTATCAGGGT TATTGTCTCA TGAGCGGATA
     2701 CATATTTGAA TGTATTTAGA AAAATAAACA AATAGGGGTT CCGCGCACCT GCACCAGTCA
     2761 GTAAAACGAC GGCCAGTAGT CAAAAGCCTC CGACCGGAGG CTTTTGACTT GGTTCAGGTG
     2821 GAGTGGGAGA ACGATGATGC TCACTCTCGG GTCTCA
//