LOCUS       BBF10K_003424           2796 bp    DNA     circular     29-Apr-2025
ACCESSION   BBF10K_003424
VERSION     BBF10K_003424.1  
FEATURES             Location/Qualifiers
     CDS             5..676
                     /label=T2T3_AmilCP
     CDS             complement(1719..2578)
                     /label=AmpR
     promoter        complement(2580..2683)
                     /label=AmpR promoter
     primer_bind     2702..2718
                     /label=M13 fwd
     primer_bind     complement(857..872)
                     /label=M13 rev
     rep_origin      complement(940..1527)
                     /label=ColE1
     primer_bind     774..792
                     /label=SLiCE-for
     primer_bind     complement(2746..2763)
                     /label=SLiCE-rev
     misc_feature    1531..1720
                     /label=spacer
     CDS             891..941
                     /label=spacer
     terminator      807..854
                     /label=T7 terminator
     terminator      2719..2750
                     /label=tonB terminator
     misc_feature    769..2769
                     /label=pOpen_v3 backbone
     misc_feature    2790..2795
                     /label=BsaI_site
     misc_feature    complement(682..687)
                     /label=BsaI_site
     misc_feature    1..4
                     /label=GTCA
     misc_feature    677..680
                     /label=TCCA
ORIGIN
        1 GTCAGTAATT GCGAAACAAA TGACATATAA AGTTTATATG TCAGGTACGG TTAATGGTCA
       61 TTACTTCGAA GTTGAAGGTG ACGGTAAGGG TAAACCATAC GAGGGTGAAC AAACTGTTAA
      121 ATTAACAGTA ACAAAAGGTG GCCCTTTACC ATTTGCTTGG GATATCTTAT CTCCACAATG
      181 TCAATACGGT TCAATCCCAT TTACTAAGTA TCCAGAAGAT ATTCCGGACT ATGTAAAACA
      241 GTCTTTTCCT GAAGGTTACA CTTGGGAACG TATTATGAAC TTCGAGGACG GTGCAGTATG
      301 TACGGTATCT AATGATTCTT CTATTCAGGG TAACTGTTTC ATTTATCATG TAAAATTTTC
      361 TGGTTTAAAT TTTCCACCAA ACGGGCCAGT AATGCAAAAA AAAACACAAG GTTGGGAACC
      421 AAATACTGAA CGTTTATTCG CACGTGATGG TATGCTTTTA GGTAACAACT TTATGGCGTT
      481 GAAATTAGAG GGCGGTGGTC ATTACTTGTG TGAGTTCAAG ACAACTTATA AAGCTAAAAA
      541 ACCGGTAAAA ATGCCAGGTT ACCACTACGT AGATCGTAAA TTAGATGTTA CGAACCATAA
      601 CAAAGACTAT ACTTCTGTAG AACAATGCGA AATTAGTATT GCTCGCAAAC CAGTAGTTGC
      661 CGGCGGTGGC GGTGGATCCA CGAGACCACA GCCTCTGTGT TGTCTGATGT CCTAAAACAT
      721 AAAGACGACT AGGGATAACA GGGTAATTCG CTATTTATTG TCATCGTCCG CTGAGGTGTC
      781 AATCGTCGGA GCCGCTGAGC AATAACTAGC ATAACCCCTT GGGGCCTCTA AACGGGTCTT
      841 GAGGGGTTTT TTGCATGGTC ATAGCTGTTT CCTGAGAGCT TGGCAGGTGA TGACACACAT
      901 TAACAAATTT CGTGAGGAGT CTCCAGAAGA ATGCCATTAA TTTCCATAGG CTCCGCCCCC
      961 CTGACGAGCA TCACAAAAAT CGACGCTCAA GTCAGAGGTG GCGAAACCCG ACAGGACTAT
     1021 AAAGATACCA GGCGTTTCCC CCTGGAAGCT CCCTCGTGCG CTCTCCTGTT CCGACCCTGC
     1081 CGCTTACCGG ATACCTGTCC GCCTTTCTCC CTTCGGGAAG CGTGGCGCTT TCTCATAGCT
     1141 CACGCTGTAG GTATCTCAGT TCGGTGTAGG TCGTTCGCTC CAAGCTGGGC TGTGTGCACG
     1201 AACCCCCCGT TCAGCCCGAC CGCTGCGCCT TATCCGGTAA CTATCGTCTT GAGTCCAACC
     1261 CGGTAAGACA CGACTTATCG CCACTGGCAG CAGCCACTGG TAACAGGATT AGCAGAGCGA
     1321 GGTATGTAGG CGGTGCTACA GAGTTCTTGA AGTGGTGGCC TAACTACGGC TACACTAGAA
     1381 GAACAGTATT TGGTATCTGC GCTCTGCTGA AGCCAGTTAC CTTCGGAAAA AGAGTTGGTA
     1441 GCTCTTGATC CGGCAAACAA ACCACCGCTG GTAGCGGTGG TTTTTTTGTT TGCAAGCAGC
     1501 AGATTACGCG CAGAAAAAAA GGATCTCAAG AAGGCCTACT ATTAGCAACA ACGATCCTTT
     1561 GATCTTTTCT ACGGGGTCTG ACGCTCAGTG GAACGAAAAC TCACGTTAAG GGATTTTGGT
     1621 CATGAGATTA TCAAAAAGGA TCTTCACCTA GATCCTTTTA AATTAAAAAT GAAGTTTTAA
     1681 ATCAATCTAA AGTATATATG AGTAAACTTG GTCTGACAGT TACCAATGCT TAATCAGTGA
     1741 GGCACCTATC TCAGCGATCT GTCTATTTCG TTCATCCATA GTTGCCTGAC TCCCCGTCGT
     1801 GTAGATAACT ACGATACGGG AGGGCTTACC ATCTGGCCCC AGTGCTGCAA TGATACCGCG
     1861 AGAACCACGC TCACCGGCTC CAGATTTATC AGCAATAAAC CAGCCAGCCG GAAGGGCCGA
     1921 GCGCAGAAGT GGTCCTGCAA CTTTATCCGC CTCCATCCAG TCTATTAATT GTTGCCGGGA
     1981 AGCTAGAGTA AGTAGTTCGC CAGTTAATAG TTTGCGCAAC GTTGTTGCCA TTGCTACAGG
     2041 CATCGTGGTG TCACGCTCGT CGTTTGGTAT GGCTTCATTC AGCTCCGGTT CCCAACGATC
     2101 AAGGCGAGTT ACATGATCCC CCATGTTGTG CAAAAAAGCG GTTAGCTCCT TCGGTCCTCC
     2161 GATCGTTGTC AGAAGTAAGT TGGCCGCAGT GTTATCACTC ATGGTTATGG CAGCACTGCA
     2221 TAATTCTCTT ACTGTCATGC CATCCGTAAG ATGCTTTTCT GTGACTGGTG AGTACTCAAC
     2281 CAAGTCATTC TGAGAATAGT GTATGCGGCG ACCGAGTTGC TCTTGCCCGG CGTCAATACG
     2341 GGATAATACC GCGCCACATA GCAGAACTTT AAAAGTGCTC ATCATTGGAA AACGTTCTTC
     2401 GGGGCGAAAA CTCTCAAGGA TCTTACCGCT GTTGAGATCC AGTTCGATGT AACCCACTCG
     2461 TGCACCCAAC TGATCTTCAG CATCTTTTAC TTTCACCAGC GTTTCTGGGT GAGCAAAAAC
     2521 AGGAAGGCAA AATGCCGCAA AAAAGGGAAT AAGGGCGACA CGGAAATGTT GAATACTCAT
     2581 ACTCTTCCTT TTTCAATATT ATTGAAGCAT TTATCAGGGT TATTGTCTCA TGAGCGGATA
     2641 CATATTTGAA TGTATTTAGA AAAATAAACA AATAGGGGTT CCGCGCACCT GCACCAGTCA
     2701 GTAAAACGAC GGCCAGTAGT CAAAAGCCTC CGACCGGAGG CTTTTGACTT GGTTCAGGTG
     2761 GAGTGGGAGA ACGATGATGC TCACTCTCGG GTCTCA
//