LOCUS       BBF10K_003425           2417 bp    DNA     circular     29-Apr-2025
ACCESSION   BBF10K_003425
VERSION     BBF10K_003425.1  
FEATURES             Location/Qualifiers
     CDS             5..37
                     /label=T2T3_GS-flexible
     CDS             complement(1210..2069)
                     /label=AmpR
     promoter        complement(2071..2174)
                     /label=AmpR promoter
     primer_bind     2193..2209
                     /label=M13 fwd
     primer_bind     complement(348..363)
                     /label=M13 rev
     rep_origin      complement(431..1018)
                     /label=ColE1
     primer_bind     265..283
                     /label=SLiCE-for
     primer_bind     complement(2237..2254)
                     /label=SLiCE-rev
     misc_feature    1022..1211
                     /label=spacer
     CDS             382..432
                     /label=spacer
     terminator      298..345
                     /label=T7 terminator
     terminator      2210..2241
                     /label=tonB terminator
     misc_feature    260..2260
                     /label=pOpen_v3 backbone
     misc_feature    2411..2416
                     /label=BsaI_site
     misc_feature    complement(43..48)
                     /label=BsaI_site
     misc_feature    1..4
                     /label=GTCA
     misc_feature    38..41
                     /label=TCCA
ORIGIN
        1 GTCAGGAGGT GGCTCTGGCG GTGGATCAGG TGGAGGCTCC ACGAGACCCC ATTGCCTCTT
       61 AGACACGCCG TTAGAGTAAT TATGTAAACT TTGCGCGGCT TGACTACGAC TTGTTCAGTT
      121 ACGTCCGAGG GCACAATCTT ATTCCCATTT GTATGTTCAG CTATCTTCTA CCCATCCCAG
      181 GTTCCTAGAC CTCCTGGATT TCCATCAGTG ATTTGCGAGT TAGGGATAAC AGGGTAATAA
      241 CCCGTTACCG CTGACGAGGC GCTGAGGTGT CAATCGTCGG AGCCGCTGAG CAATAACTAG
      301 CATAACCCCT TGGGGCCTCT AAACGGGTCT TGAGGGGTTT TTTGCATGGT CATAGCTGTT
      361 TCCTGAGAGC TTGGCAGGTG ATGACACACA TTAACAAATT TCGTGAGGAG TCTCCAGAAG
      421 AATGCCATTA ATTTCCATAG GCTCCGCCCC CCTGACGAGC ATCACAAAAA TCGACGCTCA
      481 AGTCAGAGGT GGCGAAACCC GACAGGACTA TAAAGATACC AGGCGTTTCC CCCTGGAAGC
      541 TCCCTCGTGC GCTCTCCTGT TCCGACCCTG CCGCTTACCG GATACCTGTC CGCCTTTCTC
      601 CCTTCGGGAA GCGTGGCGCT TTCTCATAGC TCACGCTGTA GGTATCTCAG TTCGGTGTAG
      661 GTCGTTCGCT CCAAGCTGGG CTGTGTGCAC GAACCCCCCG TTCAGCCCGA CCGCTGCGCC
      721 TTATCCGGTA ACTATCGTCT TGAGTCCAAC CCGGTAAGAC ACGACTTATC GCCACTGGCA
      781 GCAGCCACTG GTAACAGGAT TAGCAGAGCG AGGTATGTAG GCGGTGCTAC AGAGTTCTTG
      841 AAGTGGTGGC CTAACTACGG CTACACTAGA AGAACAGTAT TTGGTATCTG CGCTCTGCTG
      901 AAGCCAGTTA CCTTCGGAAA AAGAGTTGGT AGCTCTTGAT CCGGCAAACA AACCACCGCT
      961 GGTAGCGGTG GTTTTTTTGT TTGCAAGCAG CAGATTACGC GCAGAAAAAA AGGATCTCAA
     1021 GAAGGCCTAC TATTAGCAAC AACGATCCTT TGATCTTTTC TACGGGGTCT GACGCTCAGT
     1081 GGAACGAAAA CTCACGTTAA GGGATTTTGG TCATGAGATT ATCAAAAAGG ATCTTCACCT
     1141 AGATCCTTTT AAATTAAAAA TGAAGTTTTA AATCAATCTA AAGTATATAT GAGTAAACTT
     1201 GGTCTGACAG TTACCAATGC TTAATCAGTG AGGCACCTAT CTCAGCGATC TGTCTATTTC
     1261 GTTCATCCAT AGTTGCCTGA CTCCCCGTCG TGTAGATAAC TACGATACGG GAGGGCTTAC
     1321 CATCTGGCCC CAGTGCTGCA ATGATACCGC GAGAACCACG CTCACCGGCT CCAGATTTAT
     1381 CAGCAATAAA CCAGCCAGCC GGAAGGGCCG AGCGCAGAAG TGGTCCTGCA ACTTTATCCG
     1441 CCTCCATCCA GTCTATTAAT TGTTGCCGGG AAGCTAGAGT AAGTAGTTCG CCAGTTAATA
     1501 GTTTGCGCAA CGTTGTTGCC ATTGCTACAG GCATCGTGGT GTCACGCTCG TCGTTTGGTA
     1561 TGGCTTCATT CAGCTCCGGT TCCCAACGAT CAAGGCGAGT TACATGATCC CCCATGTTGT
     1621 GCAAAAAAGC GGTTAGCTCC TTCGGTCCTC CGATCGTTGT CAGAAGTAAG TTGGCCGCAG
     1681 TGTTATCACT CATGGTTATG GCAGCACTGC ATAATTCTCT TACTGTCATG CCATCCGTAA
     1741 GATGCTTTTC TGTGACTGGT GAGTACTCAA CCAAGTCATT CTGAGAATAG TGTATGCGGC
     1801 GACCGAGTTG CTCTTGCCCG GCGTCAATAC GGGATAATAC CGCGCCACAT AGCAGAACTT
     1861 TAAAAGTGCT CATCATTGGA AAACGTTCTT CGGGGCGAAA ACTCTCAAGG ATCTTACCGC
     1921 TGTTGAGATC CAGTTCGATG TAACCCACTC GTGCACCCAA CTGATCTTCA GCATCTTTTA
     1981 CTTTCACCAG CGTTTCTGGG TGAGCAAAAA CAGGAAGGCA AAATGCCGCA AAAAAGGGAA
     2041 TAAGGGCGAC ACGGAAATGT TGAATACTCA TACTCTTCCT TTTTCAATAT TATTGAAGCA
     2101 TTTATCAGGG TTATTGTCTC ATGAGCGGAT ACATATTTGA ATGTATTTAG AAAAATAAAC
     2161 AAATAGGGGT TCCGCGCACC TGCACCAGTC AGTAAAACGA CGGCCAGTAG TCAAAAGCCT
     2221 CCGACCGGAG GCTTTTGACT TGGTTCAGGT GGAGTGGGAG AACGATGATG CTCACTCTCG
     2281 CGGAAATTAA GTAGGTTGTG AGATGCCGAA GAGGTTCTCG TTCATCCCGT GGGACATCAA
     2341 GCTTTCCCTT GATAAAGCAT TCCGCTCGGG TATGGCAGAA AGAACGCCTT CTGAATTGTG
     2401 CTATCCTTCA GGTCTCA
//