LOCUS       BBF10K_003426           2425 bp    DNA     circular     17-Jun-2025
ACCESSION   BBF10K_003426
VERSION     BBF10K_003426.1  
FEATURES             Location/Qualifiers
     CDS             5..305
                     /label=T3C_SUMO
     CDS             complement(1348..2207)
                     /label=AmpR
     promoter        complement(2209..2312)
                     /label=AmpR promoter
     primer_bind     2331..2347
                     /label=M13 fwd
     primer_bind     complement(486..501)
                     /label=M13 rev
     rep_origin      complement(569..1156)
                     /label=ColE1
     primer_bind     403..421
                     /label=SLiCE-for
     primer_bind     complement(2375..2392)
                     /label=SLiCE-rev
     misc_feature    1160..1349
                     /label=spacer
     CDS             520..570
                     /label=spacer
     terminator      436..483
                     /label=T7 terminator
     terminator      2348..2379
                     /label=tonB terminator
     misc_feature    398..2398
                     /label=pOpen_v3 backbone
     misc_feature    2419..2424
                     /label=BsaI_site
     misc_feature    complement(311..316)
                     /label=BsaI_site
     misc_feature    1..4
                     /label=TCCA
     misc_feature    306..309
                     /label=AATG
ORIGIN
        1 TCCAGCGATA GCGAAGTGAA CCAGGAAGCG AAGCCAGAGG TGAAACCGGA GGTTAAACCA
       61 GAAACCCATA TTAACCTGAA AGTGAGCGAT GGCAGCAGCG AAATTTTTTT TAAAATTAAA
      121 AAAACCACCC CGCTGCGCCG CCTGATGGAA GCGTTTGCGA AACGCCAGGG CAAAGAAATG
      181 GATAGCCTGC GCTTTCTGTA TGATGGCATT CGCATTCAGG CGGATCAGAC CCCGGAGGAC
      241 CTGGACATGG AAGATAACGA TATTATTGAA GCGCATCGCG AACAGATTGG CGGCGCGACC
      301 TATGGAATGC GAGACCAGCC TTCGCCTTTT CTAGGATCGA TCGTGAGACA AATCATAATA
      361 GGGATAACAG GGTAATTCTA TCATACCCCC TCTCAAACGC TGAGGTGTCA ATCGTCGGAG
      421 CCGCTGAGCA ATAACTAGCA TAACCCCTTG GGGCCTCTAA ACGGGTCTTG AGGGGTTTTT
      481 TGCATGGTCA TAGCTGTTTC CTGAGAGCTT GGCAGGTGAT GACACACATT AACAAATTTC
      541 GTGAGGAGTC TCCAGAAGAA TGCCATTAAT TTCCATAGGC TCCGCCCCCC TGACGAGCAT
      601 CACAAAAATC GACGCTCAAG TCAGAGGTGG CGAAACCCGA CAGGACTATA AAGATACCAG
      661 GCGTTTCCCC CTGGAAGCTC CCTCGTGCGC TCTCCTGTTC CGACCCTGCC GCTTACCGGA
      721 TACCTGTCCG CCTTTCTCCC TTCGGGAAGC GTGGCGCTTT CTCATAGCTC ACGCTGTAGG
      781 TATCTCAGTT CGGTGTAGGT CGTTCGCTCC AAGCTGGGCT GTGTGCACGA ACCCCCCGTT
      841 CAGCCCGACC GCTGCGCCTT ATCCGGTAAC TATCGTCTTG AGTCCAACCC GGTAAGACAC
      901 GACTTATCGC CACTGGCAGC AGCCACTGGT AACAGGATTA GCAGAGCGAG GTATGTAGGC
      961 GGTGCTACAG AGTTCTTGAA GTGGTGGCCT AACTACGGCT ACACTAGAAG AACAGTATTT
     1021 GGTATCTGCG CTCTGCTGAA GCCAGTTACC TTCGGAAAAA GAGTTGGTAG CTCTTGATCC
     1081 GGCAAACAAA CCACCGCTGG TAGCGGTGGT TTTTTTGTTT GCAAGCAGCA GATTACGCGC
     1141 AGAAAAAAAG GATCTCAAGA AGGCCTACTA TTAGCAACAA CGATCCTTTG ATCTTTTCTA
     1201 CGGGGTCTGA CGCTCAGTGG AACGAAAACT CACGTTAAGG GATTTTGGTC ATGAGATTAT
     1261 CAAAAAGGAT CTTCACCTAG ATCCTTTTAA ATTAAAAATG AAGTTTTAAA TCAATCTAAA
     1321 GTATATATGA GTAAACTTGG TCTGACAGTT ACCAATGCTT AATCAGTGAG GCACCTATCT
     1381 CAGCGATCTG TCTATTTCGT TCATCCATAG TTGCCTGACT CCCCGTCGTG TAGATAACTA
     1441 CGATACGGGA GGGCTTACCA TCTGGCCCCA GTGCTGCAAT GATACCGCGA GAACCACGCT
     1501 CACCGGCTCC AGATTTATCA GCAATAAACC AGCCAGCCGG AAGGGCCGAG CGCAGAAGTG
     1561 GTCCTGCAAC TTTATCCGCC TCCATCCAGT CTATTAATTG TTGCCGGGAA GCTAGAGTAA
     1621 GTAGTTCGCC AGTTAATAGT TTGCGCAACG TTGTTGCCAT TGCTACAGGC ATCGTGGTGT
     1681 CACGCTCGTC GTTTGGTATG GCTTCATTCA GCTCCGGTTC CCAACGATCA AGGCGAGTTA
     1741 CATGATCCCC CATGTTGTGC AAAAAAGCGG TTAGCTCCTT CGGTCCTCCG ATCGTTGTCA
     1801 GAAGTAAGTT GGCCGCAGTG TTATCACTCA TGGTTATGGC AGCACTGCAT AATTCTCTTA
     1861 CTGTCATGCC ATCCGTAAGA TGCTTTTCTG TGACTGGTGA GTACTCAACC AAGTCATTCT
     1921 GAGAATAGTG TATGCGGCGA CCGAGTTGCT CTTGCCCGGC GTCAATACGG GATAATACCG
     1981 CGCCACATAG CAGAACTTTA AAAGTGCTCA TCATTGGAAA ACGTTCTTCG GGGCGAAAAC
     2041 TCTCAAGGAT CTTACCGCTG TTGAGATCCA GTTCGATGTA ACCCACTCGT GCACCCAACT
     2101 GATCTTCAGC ATCTTTTACT TTCACCAGCG TTTCTGGGTG AGCAAAAACA GGAAGGCAAA
     2161 ATGCCGCAAA AAAGGGAATA AGGGCGACAC GGAAATGTTG AATACTCATA CTCTTCCTTT
     2221 TTCAATATTA TTGAAGCATT TATCAGGGTT ATTGTCTCAT GAGCGGATAC ATATTTGAAT
     2281 GTATTTAGAA AAATAAACAA ATAGGGGTTC CGCGCACCTG CACCAGTCAG TAAAACGACG
     2341 GCCAGTAGTC AAAAGCCTCC GACCGGAGGC TTTTGACTTG GTTCAGGTGG AGTGGGAGAA
     2401 CGATGATGCT CACTCTCGGG TCTCA
//