LOCUS       BBF10K_003427           2409 bp    DNA     circular     29-Apr-2025
ACCESSION   BBF10K_003427
VERSION     BBF10K_003427.1  
FEATURES             Location/Qualifiers
     CDS             5..29
                     /label=T3C_TEV
     CDS             complement(1202..2061)
                     /label=AmpR
     promoter        complement(2063..2166)
                     /label=AmpR promoter
     primer_bind     2185..2201
                     /label=M13 fwd
     primer_bind     complement(340..355)
                     /label=M13 rev
     rep_origin      complement(423..1010)
                     /label=ColE1
     primer_bind     257..275
                     /label=SLiCE-for
     primer_bind     complement(2229..2246)
                     /label=SLiCE-rev
     misc_feature    1014..1203
                     /label=spacer
     CDS             374..424
                     /label=spacer
     terminator      290..337
                     /label=T7 terminator
     terminator      2202..2233
                     /label=tonB terminator
     misc_feature    252..2252
                     /label=pOpen_v3 backbone
     misc_feature    2403..2408
                     /label=BsaI_site
     misc_feature    complement(35..40)
                     /label=BsaI_site
     misc_feature    1..4
                     /label=TCCA
     misc_feature    30..33
                     /label=AATG
ORIGIN
        1 TCCATGGAAA ATCTTTATTT TCAAGGTGGA ATGCGAGACC GAAAACCTAT GGACTTTGTT
       61 TCGGGTAGCA CCAGAAATCT AAACCATGTG AATGTGAACG TGGCGTGCGT ACACCTTAAT
      121 CTCCGGTTCA TGCTAAGGAT GTGGCTGCAT GCTATGTTGA TACACCTACA AGCTAGTTGT
      181 CGTCATGGCA TAATGTCCTT CGCCATCTTC CTTAGGGATA ACAGGGTAAT CCCCGATGTA
      241 CAATCTGGAT GCGCTGAGGT GTCAATCGTC GGAGCCGCTG AGCAATAACT AGCATAACCC
      301 CTTGGGGCCT CTAAACGGGT CTTGAGGGGT TTTTTGCATG GTCATAGCTG TTTCCTGAGA
      361 GCTTGGCAGG TGATGACACA CATTAACAAA TTTCGTGAGG AGTCTCCAGA AGAATGCCAT
      421 TAATTTCCAT AGGCTCCGCC CCCCTGACGA GCATCACAAA AATCGACGCT CAAGTCAGAG
      481 GTGGCGAAAC CCGACAGGAC TATAAAGATA CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT
      541 GCGCTCTCCT GTTCCGACCC TGCCGCTTAC CGGATACCTG TCCGCCTTTC TCCCTTCGGG
      601 AAGCGTGGCG CTTTCTCATA GCTCACGCTG TAGGTATCTC AGTTCGGTGT AGGTCGTTCG
      661 CTCCAAGCTG GGCTGTGTGC ACGAACCCCC CGTTCAGCCC GACCGCTGCG CCTTATCCGG
      721 TAACTATCGT CTTGAGTCCA ACCCGGTAAG ACACGACTTA TCGCCACTGG CAGCAGCCAC
      781 TGGTAACAGG ATTAGCAGAG CGAGGTATGT AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG
      841 GCCTAACTAC GGCTACACTA GAAGAACAGT ATTTGGTATC TGCGCTCTGC TGAAGCCAGT
      901 TACCTTCGGA AAAAGAGTTG GTAGCTCTTG ATCCGGCAAA CAAACCACCG CTGGTAGCGG
      961 TGGTTTTTTT GTTTGCAAGC AGCAGATTAC GCGCAGAAAA AAAGGATCTC AAGAAGGCCT
     1021 ACTATTAGCA ACAACGATCC TTTGATCTTT TCTACGGGGT CTGACGCTCA GTGGAACGAA
     1081 AACTCACGTT AAGGGATTTT GGTCATGAGA TTATCAAAAA GGATCTTCAC CTAGATCCTT
     1141 TTAAATTAAA AATGAAGTTT TAAATCAATC TAAAGTATAT ATGAGTAAAC TTGGTCTGAC
     1201 AGTTACCAAT GCTTAATCAG TGAGGCACCT ATCTCAGCGA TCTGTCTATT TCGTTCATCC
     1261 ATAGTTGCCT GACTCCCCGT CGTGTAGATA ACTACGATAC GGGAGGGCTT ACCATCTGGC
     1321 CCCAGTGCTG CAATGATACC GCGAGAACCA CGCTCACCGG CTCCAGATTT ATCAGCAATA
     1381 AACCAGCCAG CCGGAAGGGC CGAGCGCAGA AGTGGTCCTG CAACTTTATC CGCCTCCATC
     1441 CAGTCTATTA ATTGTTGCCG GGAAGCTAGA GTAAGTAGTT CGCCAGTTAA TAGTTTGCGC
     1501 AACGTTGTTG CCATTGCTAC AGGCATCGTG GTGTCACGCT CGTCGTTTGG TATGGCTTCA
     1561 TTCAGCTCCG GTTCCCAACG ATCAAGGCGA GTTACATGAT CCCCCATGTT GTGCAAAAAA
     1621 GCGGTTAGCT CCTTCGGTCC TCCGATCGTT GTCAGAAGTA AGTTGGCCGC AGTGTTATCA
     1681 CTCATGGTTA TGGCAGCACT GCATAATTCT CTTACTGTCA TGCCATCCGT AAGATGCTTT
     1741 TCTGTGACTG GTGAGTACTC AACCAAGTCA TTCTGAGAAT AGTGTATGCG GCGACCGAGT
     1801 TGCTCTTGCC CGGCGTCAAT ACGGGATAAT ACCGCGCCAC ATAGCAGAAC TTTAAAAGTG
     1861 CTCATCATTG GAAAACGTTC TTCGGGGCGA AAACTCTCAA GGATCTTACC GCTGTTGAGA
     1921 TCCAGTTCGA TGTAACCCAC TCGTGCACCC AACTGATCTT CAGCATCTTT TACTTTCACC
     1981 AGCGTTTCTG GGTGAGCAAA AACAGGAAGG CAAAATGCCG CAAAAAAGGG AATAAGGGCG
     2041 ACACGGAAAT GTTGAATACT CATACTCTTC CTTTTTCAAT ATTATTGAAG CATTTATCAG
     2101 GGTTATTGTC TCATGAGCGG ATACATATTT GAATGTATTT AGAAAAATAA ACAAATAGGG
     2161 GTTCCGCGCA CCTGCACCAG TCAGTAAAAC GACGGCCAGT AGTCAAAAGC CTCCGACCGG
     2221 AGGCTTTTGA CTTGGTTCAG GTGGAGTGGG AGAACGATGA TGCTCACTCT CGTCGATGCA
     2281 CACTCCCATC TTTGAAAACG CATAAACATA CAAGTGGACA GATAATGGGT ACGGGCTTCT
     2341 AATACATCCA ACACTCTACG TCCTCTTCAA GAGCTAGAAG AGCACCCTGC AGTTGGAAAA
     2401 GAGGTCTCA
//