LOCUS       BBF10K_003428           2656 bp    DNA     circular     29-Apr-2025
ACCESSION   BBF10K_003428
VERSION     BBF10K_003428.1  
FEATURES             Location/Qualifiers
     CDS             5..536
                     /label=T3C_int_∆I
     CDS             complement(1579..2438)
                     /label=AmpR
     promoter        complement(2440..2543)
                     /label=AmpR promoter
     primer_bind     2562..2578
                     /label=M13 fwd
     primer_bind     complement(717..732)
                     /label=M13 rev
     rep_origin      complement(800..1387)
                     /label=ColE1
     primer_bind     634..652
                     /label=SLiCE-for
     primer_bind     complement(2606..2623)
                     /label=SLiCE-rev
     misc_feature    1391..1580
                     /label=spacer
     CDS             751..801
                     /label=spacer
     terminator      667..714
                     /label=T7 terminator
     terminator      2579..2610
                     /label=tonB terminator
     misc_feature    629..2629
                     /label=pOpen_v3 backbone
     misc_feature    2650..2655
                     /label=BsaI_site
     misc_feature    complement(542..547)
                     /label=BsaI_site
     misc_feature    1..4
                     /label=TCCA
     misc_feature    537..540
                     /label=AATG
ORIGIN
        1 TCCATGATCT CAGAATTCGC ATTGGCCGAG GGAACTCGTA TCTTCGATCC TGTAACTGGT
       61 ACTACTCATC GCATTGAAGA TGTTGTGGGA GGGCGTAAAC CTATCCATGT AGTCGCAGCT
      121 GCGAAGGACG GCACCCTGCA CGCACGCCCT GTGGTATCCT GGTTTGATCA GGGCACTCGC
      181 GATGTCATCG GTCTGCGCAT TGCGGGAGGT GCAATTCTGT GGGCTACTCC GGACCACAAG
      241 GTCCTGACAG AGTATGGGTG GCGTGCCGCT GGGGAGCTGC GCAAAGGAGA TCGTGTGGCC
      301 CAGCCACGTC GTTTTGACGG ATTTGGCGAT AGTGCCCCAA TCCCAGCACG TGTCCAGGCG
      361 CTGGCTGATG CTCTGGATGA TAAGTTCTTA CATGACATGC TTGCCGAAGA ACTGCGCTAT
      421 TCTGTGATTC GTGAGGTGCT TCCTACTCGT CGTGCTCGCA CGTTCGGACT GGAAGTTGAA
      481 GAACTGCATA CACTTGTCGC CGAGGGTGTC GTCGTGCACA ACGGCGGTGG CGGTGGAATG
      541 CGAGACCACC GATAGTTCCA GCTAGCATGC CGTCATTACA TGCTTGGTGT AGGGATAACA
      601 GGGTAATTAC AAAGTCGCGA GCTATGTACG CTGAGGTGTC AATCGTCGGA GCCGCTGAGC
      661 AATAACTAGC ATAACCCCTT GGGGCCTCTA AACGGGTCTT GAGGGGTTTT TTGCATGGTC
      721 ATAGCTGTTT CCTGAGAGCT TGGCAGGTGA TGACACACAT TAACAAATTT CGTGAGGAGT
      781 CTCCAGAAGA ATGCCATTAA TTTCCATAGG CTCCGCCCCC CTGACGAGCA TCACAAAAAT
      841 CGACGCTCAA GTCAGAGGTG GCGAAACCCG ACAGGACTAT AAAGATACCA GGCGTTTCCC
      901 CCTGGAAGCT CCCTCGTGCG CTCTCCTGTT CCGACCCTGC CGCTTACCGG ATACCTGTCC
      961 GCCTTTCTCC CTTCGGGAAG CGTGGCGCTT TCTCATAGCT CACGCTGTAG GTATCTCAGT
     1021 TCGGTGTAGG TCGTTCGCTC CAAGCTGGGC TGTGTGCACG AACCCCCCGT TCAGCCCGAC
     1081 CGCTGCGCCT TATCCGGTAA CTATCGTCTT GAGTCCAACC CGGTAAGACA CGACTTATCG
     1141 CCACTGGCAG CAGCCACTGG TAACAGGATT AGCAGAGCGA GGTATGTAGG CGGTGCTACA
     1201 GAGTTCTTGA AGTGGTGGCC TAACTACGGC TACACTAGAA GAACAGTATT TGGTATCTGC
     1261 GCTCTGCTGA AGCCAGTTAC CTTCGGAAAA AGAGTTGGTA GCTCTTGATC CGGCAAACAA
     1321 ACCACCGCTG GTAGCGGTGG TTTTTTTGTT TGCAAGCAGC AGATTACGCG CAGAAAAAAA
     1381 GGATCTCAAG AAGGCCTACT ATTAGCAACA ACGATCCTTT GATCTTTTCT ACGGGGTCTG
     1441 ACGCTCAGTG GAACGAAAAC TCACGTTAAG GGATTTTGGT CATGAGATTA TCAAAAAGGA
     1501 TCTTCACCTA GATCCTTTTA AATTAAAAAT GAAGTTTTAA ATCAATCTAA AGTATATATG
     1561 AGTAAACTTG GTCTGACAGT TACCAATGCT TAATCAGTGA GGCACCTATC TCAGCGATCT
     1621 GTCTATTTCG TTCATCCATA GTTGCCTGAC TCCCCGTCGT GTAGATAACT ACGATACGGG
     1681 AGGGCTTACC ATCTGGCCCC AGTGCTGCAA TGATACCGCG AGAACCACGC TCACCGGCTC
     1741 CAGATTTATC AGCAATAAAC CAGCCAGCCG GAAGGGCCGA GCGCAGAAGT GGTCCTGCAA
     1801 CTTTATCCGC CTCCATCCAG TCTATTAATT GTTGCCGGGA AGCTAGAGTA AGTAGTTCGC
     1861 CAGTTAATAG TTTGCGCAAC GTTGTTGCCA TTGCTACAGG CATCGTGGTG TCACGCTCGT
     1921 CGTTTGGTAT GGCTTCATTC AGCTCCGGTT CCCAACGATC AAGGCGAGTT ACATGATCCC
     1981 CCATGTTGTG CAAAAAAGCG GTTAGCTCCT TCGGTCCTCC GATCGTTGTC AGAAGTAAGT
     2041 TGGCCGCAGT GTTATCACTC ATGGTTATGG CAGCACTGCA TAATTCTCTT ACTGTCATGC
     2101 CATCCGTAAG ATGCTTTTCT GTGACTGGTG AGTACTCAAC CAAGTCATTC TGAGAATAGT
     2161 GTATGCGGCG ACCGAGTTGC TCTTGCCCGG CGTCAATACG GGATAATACC GCGCCACATA
     2221 GCAGAACTTT AAAAGTGCTC ATCATTGGAA AACGTTCTTC GGGGCGAAAA CTCTCAAGGA
     2281 TCTTACCGCT GTTGAGATCC AGTTCGATGT AACCCACTCG TGCACCCAAC TGATCTTCAG
     2341 CATCTTTTAC TTTCACCAGC GTTTCTGGGT GAGCAAAAAC AGGAAGGCAA AATGCCGCAA
     2401 AAAAGGGAAT AAGGGCGACA CGGAAATGTT GAATACTCAT ACTCTTCCTT TTTCAATATT
     2461 ATTGAAGCAT TTATCAGGGT TATTGTCTCA TGAGCGGATA CATATTTGAA TGTATTTAGA
     2521 AAAATAAACA AATAGGGGTT CCGCGCACCT GCACCAGTCA GTAAAACGAC GGCCAGTAGT
     2581 CAAAAGCCTC CGACCGGAGG CTTTTGACTT GGTTCAGGTG GAGTGGGAGA ACGATGATGC
     2641 TCACTCTCGG GTCTCA
//