LOCUS       BBF10K_003429           2857 bp    DNA     circular     29-Apr-2025
ACCESSION   BBF10K_003429
VERSION     BBF10K_003429.1  
FEATURES             Location/Qualifiers
     CDS             5..737
                     /label=T3C_fuGFP
     CDS             complement(1780..2639)
                     /label=AmpR
     promoter        complement(2641..2744)
                     /label=AmpR promoter
     primer_bind     2763..2779
                     /label=M13 fwd
     primer_bind     complement(918..933)
                     /label=M13 rev
     rep_origin      complement(1001..1588)
                     /label=ColE1
     primer_bind     835..853
                     /label=SLiCE-for
     primer_bind     complement(2807..2824)
                     /label=SLiCE-rev
     misc_feature    1592..1781
                     /label=spacer
     CDS             952..1002
                     /label=spacer
     terminator      868..915
                     /label=T7 terminator
     terminator      2780..2811
                     /label=tonB terminator
     misc_feature    830..2830
                     /label=pOpen_v3 backbone
     misc_feature    2851..2856
                     /label=BsaI_site
     misc_feature    complement(743..748)
                     /label=BsaI_site
     misc_feature    1..4
                     /label=TCCA
     misc_feature    738..741
                     /label=AATG
ORIGIN
        1 TCCATGGTTT CTAGCGGTGA AGATATCTTC TCAGGCCTGG TCCCGATTCT GATCGAGCTG
       61 GAGGGCGATG TGAATGGCCA CCGCTTCTCT GTTCGTGGTG AAGGTTATGG CGACGCCTCT
      121 AACGGCAAAC TGGAAATTAA GTTCATCTGC ACCACCGGTC GTCTGCCGGT TCCGTGGCCG
      181 ACGCTGGTGA CTACTCTGTC TTACGGTGTC CAGTGCTTCG CAAAATACCC GGAACACATG
      241 CGTCAGAATG ATTTTTTCAA GTCCGCCATG CCAGATGGCT ATGTTCAGGA ACGTACCATC
      301 AGCTTCAAAG AGGACGGTAC GTACAAAACC CGTGCGGAAG TTAAATTTGA AGGTGAAGCG
      361 CTGGTGAACC GTATCGACTT AAAAGGTCTG GAGTTCAAGG AAGATGGCAA CATTCTGGGC
      421 CACAAACTGG AATACAGCTT CAACAGCCAC TATGTTTATA TTACCGCGGA CAAAAATCGC
      481 AACGGCCTGG AGGCGCAGTT CCGTATCCGT CACAACGTCG ACGACGGTTC TGTCCAGCTT
      541 GCGGACCATT ACCAGCAGAA CACCCCGATC GGTGAGGGCC CAGTACTGCT CCCGGAGCAA
      601 CACTACCTGA CGACTAACTC TGTCCTGTCC AAAGACCCGC AGGAACGCCG TGATCACATG
      661 GTTCTGGTTG AATTTGTGAC CGCTGCCGGC CTGTCGCTGG GCATGGACGA ACTGTATAAA
      721 TCCGGCGGTG GCGGTGGAAT GCGAGACCAG TGTCGCTCGC AATCCTAATC GGTGGCCTGG
      781 AAGGTATGCG TAGGGATAAC AGGGTAATTG ATAAATGCCG TGCCTATTGC GCTGAGGTGT
      841 CAATCGTCGG AGCCGCTGAG CAATAACTAG CATAACCCCT TGGGGCCTCT AAACGGGTCT
      901 TGAGGGGTTT TTTGCATGGT CATAGCTGTT TCCTGAGAGC TTGGCAGGTG ATGACACACA
      961 TTAACAAATT TCGTGAGGAG TCTCCAGAAG AATGCCATTA ATTTCCATAG GCTCCGCCCC
     1021 CCTGACGAGC ATCACAAAAA TCGACGCTCA AGTCAGAGGT GGCGAAACCC GACAGGACTA
     1081 TAAAGATACC AGGCGTTTCC CCCTGGAAGC TCCCTCGTGC GCTCTCCTGT TCCGACCCTG
     1141 CCGCTTACCG GATACCTGTC CGCCTTTCTC CCTTCGGGAA GCGTGGCGCT TTCTCATAGC
     1201 TCACGCTGTA GGTATCTCAG TTCGGTGTAG GTCGTTCGCT CCAAGCTGGG CTGTGTGCAC
     1261 GAACCCCCCG TTCAGCCCGA CCGCTGCGCC TTATCCGGTA ACTATCGTCT TGAGTCCAAC
     1321 CCGGTAAGAC ACGACTTATC GCCACTGGCA GCAGCCACTG GTAACAGGAT TAGCAGAGCG
     1381 AGGTATGTAG GCGGTGCTAC AGAGTTCTTG AAGTGGTGGC CTAACTACGG CTACACTAGA
     1441 AGAACAGTAT TTGGTATCTG CGCTCTGCTG AAGCCAGTTA CCTTCGGAAA AAGAGTTGGT
     1501 AGCTCTTGAT CCGGCAAACA AACCACCGCT GGTAGCGGTG GTTTTTTTGT TTGCAAGCAG
     1561 CAGATTACGC GCAGAAAAAA AGGATCTCAA GAAGGCCTAC TATTAGCAAC AACGATCCTT
     1621 TGATCTTTTC TACGGGGTCT GACGCTCAGT GGAACGAAAA CTCACGTTAA GGGATTTTGG
     1681 TCATGAGATT ATCAAAAAGG ATCTTCACCT AGATCCTTTT AAATTAAAAA TGAAGTTTTA
     1741 AATCAATCTA AAGTATATAT GAGTAAACTT GGTCTGACAG TTACCAATGC TTAATCAGTG
     1801 AGGCACCTAT CTCAGCGATC TGTCTATTTC GTTCATCCAT AGTTGCCTGA CTCCCCGTCG
     1861 TGTAGATAAC TACGATACGG GAGGGCTTAC CATCTGGCCC CAGTGCTGCA ATGATACCGC
     1921 GAGAACCACG CTCACCGGCT CCAGATTTAT CAGCAATAAA CCAGCCAGCC GGAAGGGCCG
     1981 AGCGCAGAAG TGGTCCTGCA ACTTTATCCG CCTCCATCCA GTCTATTAAT TGTTGCCGGG
     2041 AAGCTAGAGT AAGTAGTTCG CCAGTTAATA GTTTGCGCAA CGTTGTTGCC ATTGCTACAG
     2101 GCATCGTGGT GTCACGCTCG TCGTTTGGTA TGGCTTCATT CAGCTCCGGT TCCCAACGAT
     2161 CAAGGCGAGT TACATGATCC CCCATGTTGT GCAAAAAAGC GGTTAGCTCC TTCGGTCCTC
     2221 CGATCGTTGT CAGAAGTAAG TTGGCCGCAG TGTTATCACT CATGGTTATG GCAGCACTGC
     2281 ATAATTCTCT TACTGTCATG CCATCCGTAA GATGCTTTTC TGTGACTGGT GAGTACTCAA
     2341 CCAAGTCATT CTGAGAATAG TGTATGCGGC GACCGAGTTG CTCTTGCCCG GCGTCAATAC
     2401 GGGATAATAC CGCGCCACAT AGCAGAACTT TAAAAGTGCT CATCATTGGA AAACGTTCTT
     2461 CGGGGCGAAA ACTCTCAAGG ATCTTACCGC TGTTGAGATC CAGTTCGATG TAACCCACTC
     2521 GTGCACCCAA CTGATCTTCA GCATCTTTTA CTTTCACCAG CGTTTCTGGG TGAGCAAAAA
     2581 CAGGAAGGCA AAATGCCGCA AAAAAGGGAA TAAGGGCGAC ACGGAAATGT TGAATACTCA
     2641 TACTCTTCCT TTTTCAATAT TATTGAAGCA TTTATCAGGG TTATTGTCTC ATGAGCGGAT
     2701 ACATATTTGA ATGTATTTAG AAAAATAAAC AAATAGGGGT TCCGCGCACC TGCACCAGTC
     2761 AGTAAAACGA CGGCCAGTAG TCAAAAGCCT CCGACCGGAG GCTTTTGACT TGGTTCAGGT
     2821 GGAGTGGGAG AACGATGATG CTCACTCTCG GGTCTCA
//