LOCUS       BBF10K_003430           2440 bp    DNA     circular     29-Apr-2025
ACCESSION   BBF10K_003430
VERSION     BBF10K_003430.1  
FEATURES             Location/Qualifiers
     CDS             5..41
                     /label=T3C_GS-flexible-linker
     CDS             complement(1224..2083)
                     /label=AmpR
     promoter        complement(2085..2188)
                     /label=AmpR promoter
     primer_bind     2207..2223
                     /label=M13 fwd
     primer_bind     complement(362..377)
                     /label=M13 rev
     rep_origin      complement(445..1032)
                     /label=ColE1
     primer_bind     279..297
                     /label=SLiCE-for
     primer_bind     complement(2251..2268)
                     /label=SLiCE-rev
     misc_feature    1036..1225
                     /label=spacer
     CDS             396..446
                     /label=spacer
     terminator      312..359
                     /label=T7 terminator
     terminator      2224..2255
                     /label=tonB terminator
     misc_feature    274..2274
                     /label=pOpen_v3 backbone
     misc_feature    2434..2439
                     /label=BsaI_site
     misc_feature    complement(47..52)
                     /label=BsaI_site
     misc_feature    1..4
                     /label=TCCA
     misc_feature    42..45
                     /label=AATG
ORIGIN
        1 TCCAGCGGAG GTGGCTCTGG CGGTGGATCA GGTGGAGGCT CAATGCGAGA CCTAGGTTAG
       61 CATCAGATAG ACTAACATGA ATCTTTACAC CGAAGCGAAA ACGGGTGCGT GGACTAGCGA
      121 GGAGCAAACG AAAATTCTTG GCCTGCTTGA TGTCTCGTAA TTTTCTTAGA GATGGACGAA
      181 ATGTTTCATG ACACGCTCAC GGAGTGTACT AATAACGGTT TCCGGCGGGA ATGGTAGGGA
      241 TAACAGGGTA ATAATAATGG CAGAGCTTCC ATTCGCTGAG GTGTCAATCG TCGGAGCCGC
      301 TGAGCAATAA CTAGCATAAC CCCTTGGGGC CTCTAAACGG GTCTTGAGGG GTTTTTTGCA
      361 TGGTCATAGC TGTTTCCTGA GAGCTTGGCA GGTGATGACA CACATTAACA AATTTCGTGA
      421 GGAGTCTCCA GAAGAATGCC ATTAATTTCC ATAGGCTCCG CCCCCCTGAC GAGCATCACA
      481 AAAATCGACG CTCAAGTCAG AGGTGGCGAA ACCCGACAGG ACTATAAAGA TACCAGGCGT
      541 TTCCCCCTGG AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC CCTGCCGCTT ACCGGATACC
      601 TGTCCGCCTT TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA TAGCTCACGC TGTAGGTATC
      661 TCAGTTCGGT GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT GCACGAACCC CCCGTTCAGC
      721 CCGACCGCTG CGCCTTATCC GGTAACTATC GTCTTGAGTC CAACCCGGTA AGACACGACT
      781 TATCGCCACT GGCAGCAGCC ACTGGTAACA GGATTAGCAG AGCGAGGTAT GTAGGCGGTG
      841 CTACAGAGTT CTTGAAGTGG TGGCCTAACT ACGGCTACAC TAGAAGAACA GTATTTGGTA
      901 TCTGCGCTCT GCTGAAGCCA GTTACCTTCG GAAAAAGAGT TGGTAGCTCT TGATCCGGCA
      961 AACAAACCAC CGCTGGTAGC GGTGGTTTTT TTGTTTGCAA GCAGCAGATT ACGCGCAGAA
     1021 AAAAAGGATC TCAAGAAGGC CTACTATTAG CAACAACGAT CCTTTGATCT TTTCTACGGG
     1081 GTCTGACGCT CAGTGGAACG AAAACTCACG TTAAGGGATT TTGGTCATGA GATTATCAAA
     1141 AAGGATCTTC ACCTAGATCC TTTTAAATTA AAAATGAAGT TTTAAATCAA TCTAAAGTAT
     1201 ATATGAGTAA ACTTGGTCTG ACAGTTACCA ATGCTTAATC AGTGAGGCAC CTATCTCAGC
     1261 GATCTGTCTA TTTCGTTCAT CCATAGTTGC CTGACTCCCC GTCGTGTAGA TAACTACGAT
     1321 ACGGGAGGGC TTACCATCTG GCCCCAGTGC TGCAATGATA CCGCGAGAAC CACGCTCACC
     1381 GGCTCCAGAT TTATCAGCAA TAAACCAGCC AGCCGGAAGG GCCGAGCGCA GAAGTGGTCC
     1441 TGCAACTTTA TCCGCCTCCA TCCAGTCTAT TAATTGTTGC CGGGAAGCTA GAGTAAGTAG
     1501 TTCGCCAGTT AATAGTTTGC GCAACGTTGT TGCCATTGCT ACAGGCATCG TGGTGTCACG
     1561 CTCGTCGTTT GGTATGGCTT CATTCAGCTC CGGTTCCCAA CGATCAAGGC GAGTTACATG
     1621 ATCCCCCATG TTGTGCAAAA AAGCGGTTAG CTCCTTCGGT CCTCCGATCG TTGTCAGAAG
     1681 TAAGTTGGCC GCAGTGTTAT CACTCATGGT TATGGCAGCA CTGCATAATT CTCTTACTGT
     1741 CATGCCATCC GTAAGATGCT TTTCTGTGAC TGGTGAGTAC TCAACCAAGT CATTCTGAGA
     1801 ATAGTGTATG CGGCGACCGA GTTGCTCTTG CCCGGCGTCA ATACGGGATA ATACCGCGCC
     1861 ACATAGCAGA ACTTTAAAAG TGCTCATCAT TGGAAAACGT TCTTCGGGGC GAAAACTCTC
     1921 AAGGATCTTA CCGCTGTTGA GATCCAGTTC GATGTAACCC ACTCGTGCAC CCAACTGATC
     1981 TTCAGCATCT TTTACTTTCA CCAGCGTTTC TGGGTGAGCA AAAACAGGAA GGCAAAATGC
     2041 CGCAAAAAAG GGAATAAGGG CGACACGGAA ATGTTGAATA CTCATACTCT TCCTTTTTCA
     2101 ATATTATTGA AGCATTTATC AGGGTTATTG TCTCATGAGC GGATACATAT TTGAATGTAT
     2161 TTAGAAAAAT AAACAAATAG GGGTTCCGCG CACCTGCACC AGTCAGTAAA ACGACGGCCA
     2221 GTAGTCAAAA GCCTCCGACC GGAGGCTTTT GACTTGGTTC AGGTGGAGTG GGAGAACGAT
     2281 GATGCTCACT CTCGGGTTGG CTAATCCTGG TACATTTTGT AATGAATATT CAGTAGAAAA
     2341 TTTGTGTTAG AAGGACGAGT CACCATGTAC TAAAAGCAAT AACGATCGGT GGGAGTATTC
     2401 ATTGTGGTAA GACGCTGGGT TTACATGGGA AAGGGTCTCA
//