LOCUS BBF10K_003431 4106 bp DNA circular 29-Apr-2025 ACCESSION BBF10K_003431 VERSION BBF10K_003431.1 FEATURES Location/Qualifiers CDS 5..1986 /label=CD_OMMLV CDS complement(3029..3888) /label=AmpR promoter complement(3890..3993) /label=AmpR promoter primer_bind 4012..4028 /label=M13 fwd primer_bind complement(2167..2182) /label=M13 rev rep_origin complement(2250..2837) /label=ColE1 primer_bind 2084..2102 /label=SLiCE-for primer_bind complement(4056..4073) /label=SLiCE-rev misc_feature 2841..3030 /label=spacer CDS 2201..2251 /label=spacer terminator 2117..2164 /label=T7 terminator terminator 4029..4060 /label=tonB terminator misc_feature 2079..4079 /label=pOpen_v3 backbone misc_feature 4100..4105 /label=BsaI_site misc_feature complement(1992..1997) /label=BsaI_site misc_feature 1..4 /label=AATG misc_feature 1987..1990 /label=AGGT ORIGIN 1 AATGACTCTG AACATCGAAG ATGAACATCG TCTGCACGAA ACCAGCAAAG AACCGGACGT 61 TTCTCTGGGT TCAACCTGGC TGTCTGATTT TCCGCAAGCT TGGGCCGAAA CTGGCGGTAT 121 GGGCCTGGCC GTCCGTCAGG CACCGCTGAT CATCCCACTG AAAGCGACCT CCACCCCGGT 181 GTCCATCAAA CAGTATCCAA TGTCCCAGGA AGCACGTCTG GGCATCAAGC CACATATCCA 241 ACGTCTGCTG GATGCCGGTA TCCTGGTACC GTGTCAATCG CCTTGGAACA CTCCACTGCT 301 GCCGGTGAAA AAACCGGGCA CTAACGATTA CCGTCCGGTA CAGGACCTGC GTGAAGTTAA 361 CAAACGTGTT GAAGATATTC ATCCGACCGT CCCGAATCCG TACAACCTCC TGTCCGGTCT 421 GCCGCCGAGC CATCAGTGGT ACACCGTGCT GGACCTGAAG GACGCATTCT TTTGCCTGCG 481 TCTGCACCCG ACCTCTCAGC CGCTGTTTGC GTTTGAATGG CGTGACCCGG AAATGGGTAT 541 TTCTGGCCAG CTTACATGGA CCCGCCTGCC ACAGGGTTTC AAAAACTCCC CGACCCTTTT 601 CGACGAAGCC CTGCACCGTG ACCTGGCAGA TTTTCGCATT CAGCACCCGG ACCTCATCCT 661 GCTGCAATAC ATGGACGATC TGCTGTTGGC AGCTACCAGC GAACTGGATT GCCAGCAGGG 721 TACCCGTGCG CTGCTGCAAA CCCTGGGCAA CCTGGGTTAC CGTGCTTCCG CGAAAAAGGC 781 GCAGATTTGC CAAAAACAGG TCAAATATCT GGGTTACCTG CTGAAAGAAG GCCAGCGCTG 841 GCTGACCGAG GCCCGCAAAG AAACCGTGAT GGGTCAACCG ACCCCGAAAA CCCCGCGTCA 901 ACTGCGCGAG TTTCTGGGTA CCGCTGGCTT CTGCCGTCTG TGGATTCCGG GCTTCGCTGA 961 AATGGCCGCG CCGCTGTATC CGTTAACCAA AACTGGTACT CTGTTCAACT GGGGTCCGGA 1021 CCAGCAGAAA GCCTATCAGG AGATTAAACA AGCGCTGCTG ACCGCACCGG CGCTGGGCCT 1081 GCCGGACCTG ACCAAACCAT TTGAACTGTT CGTTGACGAG AAACAAGGTT ACGCTAAAGG 1141 TGTTCTTACC CAGAAACTGG GTCCGTGGCG CCGTCCGGTA GCGTATCTGT CTAAAAAACT 1201 GGACCCGGTT GCGGCTGGTT GGCCGCCGTG TCTGCGTATG GTTGCTGCTA TTGCCGTACT 1261 GACTAAGGAT GCGGGCAAAC TGACTATGGG CCAGCCGCTG GTGATCCTGG CTCCGCATGC 1321 GGTGGAGGCC CTGGTTAAAC AGCCGCCGGA TCGCTGGCTG TCTAACGCGC GTATGACACA 1381 CTACCAGGCT CTGCTGCTGG ATACCGACCG TGTTCAGTTC GGCCCGGTCG TGGCCCTGAA 1441 CCCGGCGACC CTGCTCCCGC TGCCGGAAGA AGGTCTGCAA CACAATTGCC TGGATATTCT 1501 GGCGGAAGCC CATGGTACTC GTCCGGATCT GACCGACCAG CCTCTGCCGG ATGCAGACCA 1561 CACCTGGTAC ACTGGTGGCA GCTCCCTCCT GCAAGAGGGC CAGCGTAAGG CAGGCGCGGC 1621 TGTTACCACG GAAACCGAAG TAATCTGGGC TAAAGCCCTG CCAGCAGGAA CGTCAGCCCA 1681 ACGCGCACAA CTGATTGCTC TGACCCAGGC ACTGAAAATG GCGGAAGGTA AAAAGCTGAA 1741 CGTTTACACT AACTCCCGCT ACGCATTCGC TACCGCCCAC ATCCATGGCG AAATCTACAG 1801 ACGTCGTGGT CTGCTGACCT CTGAAGGCAA AGAAATCAAA AACAAAGATG AGATCCTGGC 1861 CCTGCTGAAA GCGCTGTTCC TGCCTAAACG TTTATCAATC ATCCACTGTC CGGGCCACCA 1921 GAAGGGCCAC TCCGCGGAAG CGCGCGGCAA CCGTATGGCA GACCAGGCAG CACGCAAAGC 1981 TGCCGGAGGT CGAGACCAGA GTCGTCCAAC CCTTGAATAC GAAAATAGGG GCTGAAGATT 2041 AGGGATAACA GGGTAATGCA TAATCCGACC CCACTCGGCG CTGAGGTGTC AATCGTCGGA 2101 GCCGCTGAGC AATAACTAGC ATAACCCCTT GGGGCCTCTA AACGGGTCTT GAGGGGTTTT 2161 TTGCATGGTC ATAGCTGTTT CCTGAGAGCT TGGCAGGTGA TGACACACAT TAACAAATTT 2221 CGTGAGGAGT CTCCAGAAGA ATGCCATTAA TTTCCATAGG CTCCGCCCCC CTGACGAGCA 2281 TCACAAAAAT CGACGCTCAA GTCAGAGGTG GCGAAACCCG ACAGGACTAT AAAGATACCA 2341 GGCGTTTCCC CCTGGAAGCT CCCTCGTGCG CTCTCCTGTT CCGACCCTGC CGCTTACCGG 2401 ATACCTGTCC GCCTTTCTCC CTTCGGGAAG CGTGGCGCTT TCTCATAGCT CACGCTGTAG 2461 GTATCTCAGT TCGGTGTAGG TCGTTCGCTC CAAGCTGGGC TGTGTGCACG AACCCCCCGT 2521 TCAGCCCGAC CGCTGCGCCT TATCCGGTAA CTATCGTCTT GAGTCCAACC CGGTAAGACA 2581 CGACTTATCG CCACTGGCAG CAGCCACTGG TAACAGGATT AGCAGAGCGA GGTATGTAGG 2641 CGGTGCTACA GAGTTCTTGA AGTGGTGGCC TAACTACGGC TACACTAGAA GAACAGTATT 2701 TGGTATCTGC GCTCTGCTGA AGCCAGTTAC CTTCGGAAAA AGAGTTGGTA GCTCTTGATC 2761 CGGCAAACAA ACCACCGCTG GTAGCGGTGG TTTTTTTGTT TGCAAGCAGC AGATTACGCG 2821 CAGAAAAAAA GGATCTCAAG AAGGCCTACT ATTAGCAACA ACGATCCTTT GATCTTTTCT 2881 ACGGGGTCTG ACGCTCAGTG GAACGAAAAC TCACGTTAAG GGATTTTGGT CATGAGATTA 2941 TCAAAAAGGA TCTTCACCTA GATCCTTTTA AATTAAAAAT GAAGTTTTAA ATCAATCTAA 3001 AGTATATATG AGTAAACTTG GTCTGACAGT TACCAATGCT TAATCAGTGA GGCACCTATC 3061 TCAGCGATCT GTCTATTTCG TTCATCCATA GTTGCCTGAC TCCCCGTCGT GTAGATAACT 3121 ACGATACGGG AGGGCTTACC ATCTGGCCCC AGTGCTGCAA TGATACCGCG AGAACCACGC 3181 TCACCGGCTC CAGATTTATC AGCAATAAAC CAGCCAGCCG GAAGGGCCGA GCGCAGAAGT 3241 GGTCCTGCAA CTTTATCCGC CTCCATCCAG TCTATTAATT GTTGCCGGGA AGCTAGAGTA 3301 AGTAGTTCGC CAGTTAATAG TTTGCGCAAC GTTGTTGCCA TTGCTACAGG CATCGTGGTG 3361 TCACGCTCGT CGTTTGGTAT GGCTTCATTC AGCTCCGGTT CCCAACGATC AAGGCGAGTT 3421 ACATGATCCC CCATGTTGTG CAAAAAAGCG GTTAGCTCCT TCGGTCCTCC GATCGTTGTC 3481 AGAAGTAAGT TGGCCGCAGT GTTATCACTC ATGGTTATGG CAGCACTGCA TAATTCTCTT 3541 ACTGTCATGC CATCCGTAAG ATGCTTTTCT GTGACTGGTG AGTACTCAAC CAAGTCATTC 3601 TGAGAATAGT GTATGCGGCG ACCGAGTTGC TCTTGCCCGG CGTCAATACG GGATAATACC 3661 GCGCCACATA GCAGAACTTT AAAAGTGCTC ATCATTGGAA AACGTTCTTC GGGGCGAAAA 3721 CTCTCAAGGA TCTTACCGCT GTTGAGATCC AGTTCGATGT AACCCACTCG TGCACCCAAC 3781 TGATCTTCAG CATCTTTTAC TTTCACCAGC GTTTCTGGGT GAGCAAAAAC AGGAAGGCAA 3841 AATGCCGCAA AAAAGGGAAT AAGGGCGACA CGGAAATGTT GAATACTCAT ACTCTTCCTT 3901 TTTCAATATT ATTGAAGCAT TTATCAGGGT TATTGTCTCA TGAGCGGATA CATATTTGAA 3961 TGTATTTAGA AAAATAAACA AATAGGGGTT CCGCGCACCT GCACCAGTCA GTAAAACGAC 4021 GGCCAGTAGT CAAAAGCCTC CGACCGGAGG CTTTTGACTT GGTTCAGGTG GAGTGGGAGA 4081 ACGATGATGC TCACTCTCGG GTCTCA //