LOCUS BBF10K_003432 3821 bp DNA circular 29-Apr-2025 ACCESSION BBF10K_003432 VERSION BBF10K_003432.1 FEATURES Location/Qualifiers CDS 5..1701 /label=CD_HIVRT CDS complement(2744..3603) /label=AmpR promoter complement(3605..3708) /label=AmpR promoter primer_bind 3727..3743 /label=M13 fwd primer_bind complement(1882..1897) /label=M13 rev rep_origin complement(1965..2552) /label=ColE1 primer_bind 1799..1817 /label=SLiCE-for primer_bind complement(3771..3788) /label=SLiCE-rev misc_feature 2556..2745 /label=spacer CDS 1916..1966 /label=spacer terminator 1832..1879 /label=T7 terminator terminator 3744..3775 /label=tonB terminator misc_feature 1794..3794 /label=pOpen_v3 backbone misc_feature 3815..3820 /label=BsaI_site misc_feature complement(1707..1712) /label=BsaI_site misc_feature 1..4 /label=AATG misc_feature 1702..1705 /label=AGGT ORIGIN 1 AATGGGCGGT AGTGGCATGC CGATCTCGCC GATTGAAACC GTGCCCGTGA AATTAAAACC 61 GGGCATGGAT GGGCCGAAAG TCAAACAGTG GCCACTGACC GAAGAGAAGA TTAAAGCGTT 121 AGTGGAGATT TGTACCGAAA TGGAAAAGGA GGGGAAGATC AGCAAGATCG GGCCAGAGAA 181 TCCGTACAAT ACCCCAGTTT TTGCGATTAA AAAGAAAGAT AGTACGAAGT GGCGCAAATT 241 GGTCGATTTT CGTGAGCTCA ATAAACGTAC GCAGGATTTC TGGGAAGTGC AGCTGGGCAT 301 ACCGCATCCG GCGGGCCTGA AGAAAAAAAA ATCAGTGACG GTCTTAGATG TTGGTGATGC 361 GTATTTTAGC GTTCCGCTGG ATGAGGACTT TCGGAAATAC ACTGCATTTA CGATCCCTTC 421 CATTAATAAC GAAACGCCGG GCATTAGGTA TCAGTACAAT GTACTTCCGC AGGGTTGGAA 481 AGGGAGCCCG GCAATCTTTC AGAGTTCTAT GACCAAAATC CTGGAACCGT TTCGTAAGCA 541 AAACCCGGAT ATTGTAATTT ATCAGTACAT GGACGATTTG TATGTCGGCT CTGACCTCGA 601 AATAGGTCAG CATCGTACAA AAATTGAAGA ACTGCGGCAG CATTTACTCC GCTGGGGGCT 661 GACCACCCCG GATAAAAAGC ACCAGAAGGA ACCTCCCTTC CTGTGGATGG GCTACGAGCT 721 GCATCCTGAT AAATGGACTG TGCAACCAAT CGTGCTGCCC GAGAAAGATT CGTGGACCGT 781 GAACGATATC CAGAAACTGG TCGGAAAGCT GAATTGGGCC TCTCAAATCT ATCCGGGTAT 841 TAAGGTACGA CAACTGTGCA AACTGCTGCG CGGTACTAAG GCCTTGACTG AGGTAATCCC 901 ACTGACAGAA GAAGCGGAAC TAGAATTGGC TGAGAACCGT GAAATCTTAA AAGAGCCGGT 961 GCACGGTGTT TATTATGACC CGAGCAAAGA CCTTATCGCG GAAATACAGA AACAGGGTCA 1021 GGGTCAATGG ACGTATCAAA TCTATCAGGA ACCATTCAAA AACCTGAAAA CAGGCAAATA 1081 TGCACGCATG CGTGGCGCGC ACACAAACGA CGTGAAACAA CTTACGGAAG CTGTGCAAAA 1141 AATCACCACC GAAAGCATTG TGATATGGGG AAAAACCCCT AAGTTTAAAC TGCCGATTCA 1201 AAAAGAAACT TGGGAAACCT GGTGGACAGA GTATTGGCAG GCGACGTGGA TTCCCGAATG 1261 GGAGTTCGTC AACACCCCCC CTCTTGTTAA ACTCTGGTAC CAGCTAGAAA AAGAACCGAT 1321 TGTTGGCGCA GAAACCTTCT ACGTAGATGG AGCCGCAAAT CGTGAAACTA AACTTGGAAA 1381 AGCCGGCTAT GTGACGAACA GAGGTCGCCA GAAAGTGGTC ACCCTGACGG ACACAACTAA 1441 TCAGAAAACG GAGCTTCAGG CCATCTATTT GGCCCTGCAA GATTCCGGAC TGGAAGTAAA 1501 TATTGTTACC GATTCCCAAT ACGCACTCGG GATCATTCAG GCGCAGCCAG ACCAATCGGA 1561 AAGCGAGCTG GTTAACCAGA TTATTGAACA GCTGATTAAA AAGGAAAAAG TCTACTTAGC 1621 TTGGGTTCCT GCGCATAAAG GTATCGGTGG TAACGAACAA GTAGATAAAC TGGTGTCAGC 1681 TGGCATTCGC AAAGTTCTGG GAGGTCGAGA CCACCATCTT GCGTCGTGAG AATAAGGGCA 1741 GGATACCTAG GCATTAGGGA TAACAGGGTA ATTGAGTCTA TAATATTATG TAACGCTGAG 1801 GTGTCAATCG TCGGAGCCGC TGAGCAATAA CTAGCATAAC CCCTTGGGGC CTCTAAACGG 1861 GTCTTGAGGG GTTTTTTGCA TGGTCATAGC TGTTTCCTGA GAGCTTGGCA GGTGATGACA 1921 CACATTAACA AATTTCGTGA GGAGTCTCCA GAAGAATGCC ATTAATTTCC ATAGGCTCCG 1981 CCCCCCTGAC GAGCATCACA AAAATCGACG CTCAAGTCAG AGGTGGCGAA ACCCGACAGG 2041 ACTATAAAGA TACCAGGCGT TTCCCCCTGG AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC 2101 CCTGCCGCTT ACCGGATACC TGTCCGCCTT TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA 2161 TAGCTCACGC TGTAGGTATC TCAGTTCGGT GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT 2221 GCACGAACCC CCCGTTCAGC CCGACCGCTG CGCCTTATCC GGTAACTATC GTCTTGAGTC 2281 CAACCCGGTA AGACACGACT TATCGCCACT GGCAGCAGCC ACTGGTAACA GGATTAGCAG 2341 AGCGAGGTAT GTAGGCGGTG CTACAGAGTT CTTGAAGTGG TGGCCTAACT ACGGCTACAC 2401 TAGAAGAACA GTATTTGGTA TCTGCGCTCT GCTGAAGCCA GTTACCTTCG GAAAAAGAGT 2461 TGGTAGCTCT TGATCCGGCA AACAAACCAC CGCTGGTAGC GGTGGTTTTT TTGTTTGCAA 2521 GCAGCAGATT ACGCGCAGAA AAAAAGGATC TCAAGAAGGC CTACTATTAG CAACAACGAT 2581 CCTTTGATCT TTTCTACGGG GTCTGACGCT CAGTGGAACG AAAACTCACG TTAAGGGATT 2641 TTGGTCATGA GATTATCAAA AAGGATCTTC ACCTAGATCC TTTTAAATTA AAAATGAAGT 2701 TTTAAATCAA TCTAAAGTAT ATATGAGTAA ACTTGGTCTG ACAGTTACCA ATGCTTAATC 2761 AGTGAGGCAC CTATCTCAGC GATCTGTCTA TTTCGTTCAT CCATAGTTGC CTGACTCCCC 2821 GTCGTGTAGA TAACTACGAT ACGGGAGGGC TTACCATCTG GCCCCAGTGC TGCAATGATA 2881 CCGCGAGAAC CACGCTCACC GGCTCCAGAT TTATCAGCAA TAAACCAGCC AGCCGGAAGG 2941 GCCGAGCGCA GAAGTGGTCC TGCAACTTTA TCCGCCTCCA TCCAGTCTAT TAATTGTTGC 3001 CGGGAAGCTA GAGTAAGTAG TTCGCCAGTT AATAGTTTGC GCAACGTTGT TGCCATTGCT 3061 ACAGGCATCG TGGTGTCACG CTCGTCGTTT GGTATGGCTT CATTCAGCTC CGGTTCCCAA 3121 CGATCAAGGC GAGTTACATG ATCCCCCATG TTGTGCAAAA AAGCGGTTAG CTCCTTCGGT 3181 CCTCCGATCG TTGTCAGAAG TAAGTTGGCC GCAGTGTTAT CACTCATGGT TATGGCAGCA 3241 CTGCATAATT CTCTTACTGT CATGCCATCC GTAAGATGCT TTTCTGTGAC TGGTGAGTAC 3301 TCAACCAAGT CATTCTGAGA ATAGTGTATG CGGCGACCGA GTTGCTCTTG CCCGGCGTCA 3361 ATACGGGATA ATACCGCGCC ACATAGCAGA ACTTTAAAAG TGCTCATCAT TGGAAAACGT 3421 TCTTCGGGGC GAAAACTCTC AAGGATCTTA CCGCTGTTGA GATCCAGTTC GATGTAACCC 3481 ACTCGTGCAC CCAACTGATC TTCAGCATCT TTTACTTTCA CCAGCGTTTC TGGGTGAGCA 3541 AAAACAGGAA GGCAAAATGC CGCAAAAAAG GGAATAAGGG CGACACGGAA ATGTTGAATA 3601 CTCATACTCT TCCTTTTTCA ATATTATTGA AGCATTTATC AGGGTTATTG TCTCATGAGC 3661 GGATACATAT TTGAATGTAT TTAGAAAAAT AAACAAATAG GGGTTCCGCG CACCTGCACC 3721 AGTCAGTAAA ACGACGGCCA GTAGTCAAAA GCCTCCGACC GGAGGCTTTT GACTTGGTTC 3781 AGGTGGAGTG GGAGAACGAT GATGCTCACT CTCGGGTCTC A //