LOCUS BBF10K_003433 3890 bp DNA circular 29-Apr-2025 ACCESSION BBF10K_003433 VERSION BBF10K_003433.1 FEATURES Location/Qualifiers CDS 5..1770 /label=CD_Bst-LF CDS complement(2813..3672) /label=AmpR promoter complement(3674..3777) /label=AmpR promoter primer_bind 3796..3812 /label=M13 fwd primer_bind complement(1951..1966) /label=M13 rev rep_origin complement(2034..2621) /label=ColE1 primer_bind 1868..1886 /label=SLiCE-for primer_bind complement(3840..3857) /label=SLiCE-rev misc_feature 2625..2814 /label=spacer CDS 1985..2035 /label=spacer terminator 1901..1948 /label=T7 terminator terminator 3813..3844 /label=tonB terminator misc_feature 1863..3863 /label=pOpen_v3 backbone misc_feature 3884..3889 /label=BsaI_site misc_feature complement(1776..1781) /label=BsaI_site misc_feature 1..4 /label=AATG misc_feature 1771..1774 /label=AGGT ORIGIN 1 AATGACCGAC GAAGGCGAAA AACCGCTGGC GGGTATGGAC TTTGCGATCG CTGATAGCGT 61 TACCGACGAG ATGCTGGCAG ATAAAGCAGC GCTAGTAGTT GAAGTTGTGG GCGATAACTA 121 CCATCATGCG CCGATCGTGG GTATCGCTTT GGCGAACGAG CGCGGCCGTT TTTTCCTGCG 181 CCCGGAAACC GCACTGGCGG ACCCGAAGTT TCTGGCTTGG CTGGGCGACG AAACGAAAAA 241 AAAGACTATG TTCGATTCTA AACGTGCAGC GGTGGCTCTC AAATGGAAAG GTATCGAATT 301 GCGCGGTGTT GTGTTCGACC TGCTGCTGGC TGCGTACCTG CTCGATCCTG CTCAGGCTGC 361 GGGTGACGTG GCAGCTGTAG CGAAAATGCA CCAGTACGAA GCCGTACGCT CTGATGAAGC 421 GGTTTATGGT AAAGGTGCGA AACGTACCGT GCCAGACGAA CCAACCCTGG CGGAACACCT 481 GGCTCGCAAA GCAGCCGCTA TCTGGGCCCT GGAGGAGCCT CTGATGGATG AACTTCGCCG 541 CAACGAACAG GACCGTCTGC TCACAGAACT GGAACAGCCG TTGGCGGGTA TTCTGGCAAA 601 CATGGAGTTC ACTGGTGTAA AAGTGGACAC TAAGCGCCTG GAACAAATGG GTGCCGAACT 661 GACTGAGCAG CTTCAGGCAG TTGAACGCCG CATCTACGAA CTGGCGGGTC AGGAGTTTAA 721 CATCAACTCT CCTAAACAGC TGGGCACCGT GTTGTTCGAC AAACTGCAAC TGCCGGTGTT 781 GAAGAAAACG AAAACCGGCT ACAGCACCTC CGCCGATGTT CTGGAAAAAC TGGCACCGCA 841 TCACGAAATC GTTGAACACA TCCTGCACTA CCGTCAGCTG GGTAAACTGC AATCTACCTA 901 CATCGAGGGC TTACTGAAAG TAGTGCACCC GGTGACCGGT AAAGTGCACA CTATGTTCAA 961 CCAGGCTCTG ACGCAGACCG GCCGCCTGTC CTCCGTTGAA CCGAACCTGC AAAACATCCC 1021 CATCCGTCTG GAAGAGGGCC GTAAGATCCG TCAGGCATTC GTGCCGTCTG AACCGGACTG 1081 GCTGATTTTC GCTGCTGATT ACTCTCAAAT CGAACTGCGT GTTCTTGCTC ACATTGCGGA 1141 GGACGATAAC CTGATTGAAG CATTCCGTCG TGGTCTGGAC ATTCACACTA AAACGGCTAT 1201 GGACATTTTC CATGTTTCCG AAGAAGATGT GACCGCTAAT ATGCGTCGTC AGGCTAAAGC 1261 GGTAAACTTT GGTATCGTAT ACGGTATCTC CGATTACGGC CTGGCACAGA ACCTGAACAT 1321 CACCCGCAAA GAAGCGGCTG AGTTTATCGA ACGTTATTTC GCCTCGTTCC CCGGTGTTAA 1381 ACAGTACATG GATAACATCG TTCAAGAAGC TAAACAGAAG GGTTATGTGA CCACCCTGCT 1441 GCACCGTCGT CGTTACCTGC CGGACATCAC CTCCCGTAAC TTCAACGTGC GTTCTTTCGC 1501 CGAACGTACG GCTATGAATA CCCCTATCCA GGGCAGCGCG GCGGACATCA TCAAAAAAGC 1561 CATGATCGAC CTGAGCGTGC GCCTGCGCGA AGAACGTTTG CAGGCTCGCC TGCTTCTGCA 1621 AGTGCACGAT GAGCTGATCC TAGAAGCTCC AAAAGAGGAA ATTGAACGTT TGTGCCGTCT 1681 GGTTCCGGAA GTTATGGAAC AGGCTGTCGC ACTGCGTGTT CCGCTTAAAG TTGATTACCA 1741 TTACGGTCCC ACTTGGTACG ACGCCAAGGG AGGTCGAGAC CACGAGTGTA GTTCCCGTCA 1801 ATCCCGTCTA CTATTTTGCG GTCTAGGGAT AACAGGGTAA TGCCGAAGAT CGCGTACGTG 1861 GCCGCTGAGG TGTCAATCGT CGGAGCCGCT GAGCAATAAC TAGCATAACC CCTTGGGGCC 1921 TCTAAACGGG TCTTGAGGGG TTTTTTGCAT GGTCATAGCT GTTTCCTGAG AGCTTGGCAG 1981 GTGATGACAC ACATTAACAA ATTTCGTGAG GAGTCTCCAG AAGAATGCCA TTAATTTCCA 2041 TAGGCTCCGC CCCCCTGACG AGCATCACAA AAATCGACGC TCAAGTCAGA GGTGGCGAAA 2101 CCCGACAGGA CTATAAAGAT ACCAGGCGTT TCCCCCTGGA AGCTCCCTCG TGCGCTCTCC 2161 TGTTCCGACC CTGCCGCTTA CCGGATACCT GTCCGCCTTT CTCCCTTCGG GAAGCGTGGC 2221 GCTTTCTCAT AGCTCACGCT GTAGGTATCT CAGTTCGGTG TAGGTCGTTC GCTCCAAGCT 2281 GGGCTGTGTG CACGAACCCC CCGTTCAGCC CGACCGCTGC GCCTTATCCG GTAACTATCG 2341 TCTTGAGTCC AACCCGGTAA GACACGACTT ATCGCCACTG GCAGCAGCCA CTGGTAACAG 2401 GATTAGCAGA GCGAGGTATG TAGGCGGTGC TACAGAGTTC TTGAAGTGGT GGCCTAACTA 2461 CGGCTACACT AGAAGAACAG TATTTGGTAT CTGCGCTCTG CTGAAGCCAG TTACCTTCGG 2521 AAAAAGAGTT GGTAGCTCTT GATCCGGCAA ACAAACCACC GCTGGTAGCG GTGGTTTTTT 2581 TGTTTGCAAG CAGCAGATTA CGCGCAGAAA AAAAGGATCT CAAGAAGGCC TACTATTAGC 2641 AACAACGATC CTTTGATCTT TTCTACGGGG TCTGACGCTC AGTGGAACGA AAACTCACGT 2701 TAAGGGATTT TGGTCATGAG ATTATCAAAA AGGATCTTCA CCTAGATCCT TTTAAATTAA 2761 AAATGAAGTT TTAAATCAAT CTAAAGTATA TATGAGTAAA CTTGGTCTGA CAGTTACCAA 2821 TGCTTAATCA GTGAGGCACC TATCTCAGCG ATCTGTCTAT TTCGTTCATC CATAGTTGCC 2881 TGACTCCCCG TCGTGTAGAT AACTACGATA CGGGAGGGCT TACCATCTGG CCCCAGTGCT 2941 GCAATGATAC CGCGAGAACC ACGCTCACCG GCTCCAGATT TATCAGCAAT AAACCAGCCA 3001 GCCGGAAGGG CCGAGCGCAG AAGTGGTCCT GCAACTTTAT CCGCCTCCAT CCAGTCTATT 3061 AATTGTTGCC GGGAAGCTAG AGTAAGTAGT TCGCCAGTTA ATAGTTTGCG CAACGTTGTT 3121 GCCATTGCTA CAGGCATCGT GGTGTCACGC TCGTCGTTTG GTATGGCTTC ATTCAGCTCC 3181 GGTTCCCAAC GATCAAGGCG AGTTACATGA TCCCCCATGT TGTGCAAAAA AGCGGTTAGC 3241 TCCTTCGGTC CTCCGATCGT TGTCAGAAGT AAGTTGGCCG CAGTGTTATC ACTCATGGTT 3301 ATGGCAGCAC TGCATAATTC TCTTACTGTC ATGCCATCCG TAAGATGCTT TTCTGTGACT 3361 GGTGAGTACT CAACCAAGTC ATTCTGAGAA TAGTGTATGC GGCGACCGAG TTGCTCTTGC 3421 CCGGCGTCAA TACGGGATAA TACCGCGCCA CATAGCAGAA CTTTAAAAGT GCTCATCATT 3481 GGAAAACGTT CTTCGGGGCG AAAACTCTCA AGGATCTTAC CGCTGTTGAG ATCCAGTTCG 3541 ATGTAACCCA CTCGTGCACC CAACTGATCT TCAGCATCTT TTACTTTCAC CAGCGTTTCT 3601 GGGTGAGCAA AAACAGGAAG GCAAAATGCC GCAAAAAAGG GAATAAGGGC GACACGGAAA 3661 TGTTGAATAC TCATACTCTT CCTTTTTCAA TATTATTGAA GCATTTATCA GGGTTATTGT 3721 CTCATGAGCG GATACATATT TGAATGTATT TAGAAAAATA AACAAATAGG GGTTCCGCGC 3781 ACCTGCACCA GTCAGTAAAA CGACGGCCAG TAGTCAAAAG CCTCCGACCG GAGGCTTTTG 3841 ACTTGGTTCA GGTGGAGTGG GAGAACGATG ATGCTCACTC TCGGGTCTCA //