LOCUS BBF10K_003434 4436 bp DNA circular 29-Apr-2025 ACCESSION BBF10K_003434 VERSION BBF10K_003434.1 FEATURES Location/Qualifiers CDS 5..2316 /label=CD_OpenVent CDS complement(3359..4218) /label=AmpR promoter complement(4220..4323) /label=AmpR promoter primer_bind 4342..4358 /label=M13 fwd primer_bind complement(2497..2512) /label=M13 rev rep_origin complement(2580..3167) /label=ColE1 primer_bind 2414..2432 /label=SLiCE-for primer_bind complement(4386..4403) /label=SLiCE-rev misc_feature 3171..3360 /label=spacer CDS 2531..2581 /label=spacer terminator 2447..2494 /label=T7 terminator terminator 4359..4390 /label=tonB terminator misc_feature 2409..4409 /label=pOpen_v3 backbone misc_feature 4430..4435 /label=BsaI_site misc_feature complement(2322..2327) /label=BsaI_site misc_feature 1..4 /label=AATG misc_feature 2317..2320 /label=AGGT ORIGIN 1 AATGATCATT GACGCTGACT ACATCACTGA AGATGGCAAA CCGATCATCC GTATTTTTAA 61 AAAAGAGAAA GGTGAATTCA AAGTCGAATA CGACCGCACC TTTCGCCCGT ACATTTACGC 121 GCTGCTGAAA GATGACAGCG CTATTGATGA AGTGAAGAAA ATTACTGCGG AACGTCATGG 181 TAAAATTGTT CGTATTACGG AGGTGGAAAA AGTCCAAAAA AAATTCCTGG GCCGTCCTAT 241 CGAGGTGTGG AAGCTGTACC TGGAACATCC TCAGGATGTT CCGGCAATTC GCGAAAAAAT 301 CCGTGAACAC CCGGCCGTCG TAGATATCTT CGAGTACGAT ATCCCGTTCG CAAAACGTTA 361 CCTGATTGAT AAAGGCCTGA CGCCGATGGA GGGCAACGAA GAACTGACCT TTCTGGCGGT 421 GGACATCGAA ACTCTGTATC ACGAAGGCGA AGAATTTGGT AAAGGCCCTA TTATCATGAT 481 TTCGTATGCG GATGAGGAAG GAGCGAAAGT GATCACATGG AAAAGCATTG ACCTGCCGTA 541 CGTTGAAGTG GTCAGCTCAG AACGCGAAAT GATTAAACGC CTCGTTAAAG TCATTCGTGA 601 AAAGGATCCG GACGTAATCA TCACCTACAA TGGTGATAAC TTCGACTTTC CGTATCTTCT 661 GAAACGTGCG GAAAAATTGG GCATTAAACT GCCGCTTGGT CGTGACAACA GCGAACCGAA 721 AATGCAGCGC ATGGGTGACA GCCTGGCGGT GGAAATTAAA GGTCGTATCC ACTTCGACCT 781 GTTCCCGGCG ATCCGTCGTA CCATCAACCT GCCGACGTAT ACCCTGGAAA CGGTTTACGA 841 AGTTATTTTC GGCAAATCCA AAGAAAAAGT TTACGCTCAC GAAATCGCGG AAGCATGGGA 901 AACCGGTAAA GGCCTGGAAC GTGTGGCAAA GTACAGCATG GAAGATGCAA AAGTGACCAG 961 CGAACTGGGG AAAGAATTCT TTCCAATGGA AGCGCAACTG GCACGCCTGG TGGGTCATCC 1021 GGTCTGGGAC GTGTCTCGTA GCTCGACTGG CAACCTGGTG GAATGGTTCC TGCTGACTAA 1081 AGCTTACGAA CGCAACGAGC TCGCACCGAA CAAACCGGAT GAACGTGAAT ACGAACGCCG 1141 TCTGCGTGAG AGCTACGAAG GCGGTTACGT GAACGAACCG GAAAAAGGCC TGTGGGAGGG 1201 TATCGTGAGC CTGGATTTCC GTAGCCTGTA CCCGTCGATT ATCATCACTC ATAACGTATC 1261 CCCAGATACG CTCAACCGTG AAAACTGTAA AGAATATGAC GTTGCGCCTC AGGTGGGTCA 1321 TCGCTTCTGC AAGGATTTTC CAGGTTTCAT CCCGAGCCTG CTGGGAAACC TGCTGGAGGA 1381 ACGTCAAAAA ATCAAAAAAC GCATGAAAGA GAGTAAAGAC CCGGTGGAAA AAAAGCTGCT 1441 GGACTACCGT CAGCGTGCTA TTAAGATCCT TGCGAACTCT TATTACGGTT ATTACGGCTA 1501 TGCAAAAGCG CGTTGGTACT GCAAAGAATG CGCTGAATCC GTTACTGCAT GGGGTCGTCA 1561 GTACATCGAC CTGGTACGTA GGGAACTGGA GAGCCGTGGC TTCAAAGTAC TGTATATCGA 1621 CACTGATGGT CTGTACGCCA CTATCCCGGG CGCAAAACAT GAAGAGATCA AAGAGAAAGC 1681 CCTGAAATTC GTCGAGTATA TAAACTCCAA ACTCCCGGGC CTGCTGGAAC TGGAATATGA 1741 AGGCTTCTAC GCGCGCGGCT TCTTTGTGAC CAAAAAAAAA TACGCTCTGA TTGATGAGGA 1801 AGGTAAAATA GTTACCCGCG GCCTGGAAAT CGTGCGCCGT GACTGGAGCG AAATCGCGAA 1861 AGAGACTCAG GCAAAAGTTC TGGAAGCAAT CCTGAAGCAC GGCAACGTTG ACGAGGCGGT 1921 GAAAATCGTA AAGGAAGTTA CCGAAAAGCT GTCGAAGTAT GAGATCCCAC CAGAAAAACT 1981 GGTTATTTAC GAGCAAATCA CCCGCCCGTT ATCAGAATAC AAAGCAATTG GCCCGCATGT 2041 AGCTGTGGCC AAACGCCTGG CCGCGAAAGG CGTAAAAGTT AAACCGGGTA TGGTAATTGG 2101 GTACATCGTC CTGAAGGGAG ATGGTCCAAT CAGCAAACGT GCCATTGCTA TTGAAGAGTT 2161 TGATCCGAAA AAACACAAAT ACGACGCCGA ATACTATATT GAAAACCAAG TTCTGCCGGC 2221 CGTTGAACGT ATCCTGCGCG CGTTCGGCTA CCGTAAGGAG GATCTGCGCT ACCAAAAAAC 2281 CAAACAGGTG GGTCTGGGTG CTTGGCTGAA ATTCGGAGGT CGAGACCACC GGTTGTACCT 2341 ATCGAGTGCC AGCTACAAAA ACGTGGACGT AGGGATAACA GGGTAATGGG CGACGAGAGG 2401 ACTGTATACG CTGAGGTGTC AATCGTCGGA GCCGCTGAGC AATAACTAGC ATAACCCCTT 2461 GGGGCCTCTA AACGGGTCTT GAGGGGTTTT TTGCATGGTC ATAGCTGTTT CCTGAGAGCT 2521 TGGCAGGTGA TGACACACAT TAACAAATTT CGTGAGGAGT CTCCAGAAGA ATGCCATTAA 2581 TTTCCATAGG CTCCGCCCCC CTGACGAGCA TCACAAAAAT CGACGCTCAA GTCAGAGGTG 2641 GCGAAACCCG ACAGGACTAT AAAGATACCA GGCGTTTCCC CCTGGAAGCT CCCTCGTGCG 2701 CTCTCCTGTT CCGACCCTGC CGCTTACCGG ATACCTGTCC GCCTTTCTCC CTTCGGGAAG 2761 CGTGGCGCTT TCTCATAGCT CACGCTGTAG GTATCTCAGT TCGGTGTAGG TCGTTCGCTC 2821 CAAGCTGGGC TGTGTGCACG AACCCCCCGT TCAGCCCGAC CGCTGCGCCT TATCCGGTAA 2881 CTATCGTCTT GAGTCCAACC CGGTAAGACA CGACTTATCG CCACTGGCAG CAGCCACTGG 2941 TAACAGGATT AGCAGAGCGA GGTATGTAGG CGGTGCTACA GAGTTCTTGA AGTGGTGGCC 3001 TAACTACGGC TACACTAGAA GAACAGTATT TGGTATCTGC GCTCTGCTGA AGCCAGTTAC 3061 CTTCGGAAAA AGAGTTGGTA GCTCTTGATC CGGCAAACAA ACCACCGCTG GTAGCGGTGG 3121 TTTTTTTGTT TGCAAGCAGC AGATTACGCG CAGAAAAAAA GGATCTCAAG AAGGCCTACT 3181 ATTAGCAACA ACGATCCTTT GATCTTTTCT ACGGGGTCTG ACGCTCAGTG GAACGAAAAC 3241 TCACGTTAAG GGATTTTGGT CATGAGATTA TCAAAAAGGA TCTTCACCTA GATCCTTTTA 3301 AATTAAAAAT GAAGTTTTAA ATCAATCTAA AGTATATATG AGTAAACTTG GTCTGACAGT 3361 TACCAATGCT TAATCAGTGA GGCACCTATC TCAGCGATCT GTCTATTTCG TTCATCCATA 3421 GTTGCCTGAC TCCCCGTCGT GTAGATAACT ACGATACGGG AGGGCTTACC ATCTGGCCCC 3481 AGTGCTGCAA TGATACCGCG AGAACCACGC TCACCGGCTC CAGATTTATC AGCAATAAAC 3541 CAGCCAGCCG GAAGGGCCGA GCGCAGAAGT GGTCCTGCAA CTTTATCCGC CTCCATCCAG 3601 TCTATTAATT GTTGCCGGGA AGCTAGAGTA AGTAGTTCGC CAGTTAATAG TTTGCGCAAC 3661 GTTGTTGCCA TTGCTACAGG CATCGTGGTG TCACGCTCGT CGTTTGGTAT GGCTTCATTC 3721 AGCTCCGGTT CCCAACGATC AAGGCGAGTT ACATGATCCC CCATGTTGTG CAAAAAAGCG 3781 GTTAGCTCCT TCGGTCCTCC GATCGTTGTC AGAAGTAAGT TGGCCGCAGT GTTATCACTC 3841 ATGGTTATGG CAGCACTGCA TAATTCTCTT ACTGTCATGC CATCCGTAAG ATGCTTTTCT 3901 GTGACTGGTG AGTACTCAAC CAAGTCATTC TGAGAATAGT GTATGCGGCG ACCGAGTTGC 3961 TCTTGCCCGG CGTCAATACG GGATAATACC GCGCCACATA GCAGAACTTT AAAAGTGCTC 4021 ATCATTGGAA AACGTTCTTC GGGGCGAAAA CTCTCAAGGA TCTTACCGCT GTTGAGATCC 4081 AGTTCGATGT AACCCACTCG TGCACCCAAC TGATCTTCAG CATCTTTTAC TTTCACCAGC 4141 GTTTCTGGGT GAGCAAAAAC AGGAAGGCAA AATGCCGCAA AAAAGGGAAT AAGGGCGACA 4201 CGGAAATGTT GAATACTCAT ACTCTTCCTT TTTCAATATT ATTGAAGCAT TTATCAGGGT 4261 TATTGTCTCA TGAGCGGATA CATATTTGAA TGTATTTAGA AAAATAAACA AATAGGGGTT 4321 CCGCGCACCT GCACCAGTCA GTAAAACGAC GGCCAGTAGT CAAAAGCCTC CGACCGGAGG 4381 CTTTTGACTT GGTTCAGGTG GAGTGGGAGA ACGATGATGC TCACTCTCGG GTCTCA //