LOCUS BBF10K_003436 3491 bp DNA circular 29-Apr-2025 ACCESSION BBF10K_003436 VERSION BBF10K_003436.1 FEATURES Location/Qualifiers CDS 5..1371 /label=CD_RNAseInhibitor CDS complement(2414..3273) /label=AmpR promoter complement(3275..3378) /label=AmpR promoter primer_bind 3397..3413 /label=M13 fwd primer_bind complement(1552..1567) /label=M13 rev rep_origin complement(1635..2222) /label=ColE1 primer_bind 1469..1487 /label=SLiCE-for primer_bind complement(3441..3458) /label=SLiCE-rev misc_feature 2226..2415 /label=spacer CDS 1586..1636 /label=spacer terminator 1502..1549 /label=T7 terminator terminator 3414..3445 /label=tonB terminator misc_feature 1464..3464 /label=pOpen_v3 backbone misc_feature 3485..3490 /label=BsaI_site misc_feature complement(1377..1382) /label=BsaI_site misc_feature 1..4 /label=AATG misc_feature 1372..1375 /label=AGGT ORIGIN 1 AATGAGTCTT GACATCCAGT GTGAGCAGCT GAGTGATGCC CGGTGGACAG AGCTCCTTCC 61 TCTGATCCAG CAATACGAAG TGGTCAGGCT GGATGACTGT GGCCTCACTG AAGTGCGGTG 121 CAAAGACATC AGCTCAGCAG TCCAAGCCAA CCCTGCCCTG ACAGAGCTCA GCCTTCGCAC 181 CAATGAACTG GGTGATGGCG GTGTGGGTCT GGTGCTCCAG GGCCTGCAAA ATCCCACTTG 241 TAAGATCCAG AAGCTGAGCC TTCAGAACTG CGGCTTGACG GAGGCTGGCT GTGGGATTCT 301 GCCTGGCATG CTGCGCTCTT TGTCTACCCT GCGTGAGCTA CATCTCAATG ATAACCCTAT 361 GGGGGACGCA GGCCTGAAGC TGCTCTGTGA AGGACTTCAG GACCCCCAGT GCCGTCTTGA 421 GAAGCTTCAG TTGGAATACT GTAACCTCAC AGCTACCAGC TGCGAGCCCC TGGCTTCAGT 481 GCTCAGGGTG AAAGCTGACT TTAAGGAGCT AGTATTGAGC AACAACGACC TCCATGAACC 541 TGGTGTCCGT ATTCTGTGCC AGGGCCTGAA GGACTCTGCC TGTCAACTGG AGTCACTCAA 601 GCTGGAGAAC TGTGGTATCA CAGCAGCCAA CTGCAAGGAT CTGTGCGATG TTGTGGCCTC 661 CAAAGCTTCG CTACAGGAAC TGGACTTGAG CAGCAACAAG CTAGGTAATG CAGGCATTGC 721 AGCACTGTGC CCAGGACTGC TGCTTCCCAG CTGTAAGCTC AGGACTCTGT GGCTCTGGGA 781 ATGTGATATC ACTGCGGAGG GCTGCAAGGA CCTGTGCCGT GTCCTCAGAG CCAAGCAGAG 841 CCTGAAGGAA CTCAGCCTTG CTTCCAATGA GCTGAAGGAT GAGGGTGCCC GGCTGCTGTG 901 TGAGAGCCTA TTAGAGCCTG GCTGTCAGCT GGAGTCACTG TGGATAAAGA CCTGTAGCCT 961 CACAGCTGCC TCCTGTCCCT ACTTCTGCTC CGTGTTGACC AAAAGCCGTT CTCTGTTGGA 1021 GCTGCAAATG AGCAGCAACC CACTGGGAGA TGAGGGAGTC CAGGAGCTTT GCAAGGCCCT 1081 GAGCCAGCCA GATACAGTGC TGCGTGAGCT TTGGTTGGGA GACTGTGATG TGACAAATAG 1141 TGGTTGCAGC AGCCTTGCCA ATGTCCTGCT GGCCAACCGC AGCCTGAGGG AACTGGACCT 1201 CAGTAACAAC TGCATGGGGG GCCCAGGTGT CCTGCAACTG CTGGAGAGCC TCAAACAGCC 1261 CAGCTGCACC CTTCAGCAGC TCGTCCTGTA TGACATTTAC TGGACAAATG AGGTGGAAGA 1321 ACAGCTTCGG GCCCTGGAGG AGGAAAGGCC TTCCCTGAGG ATCATTTCCG GAGGTCGAGA 1381 CCAGCCATCC TCTTATCTCG TGGGGGATAG TCTAAGCTTA ATACTAGGGA TAACAGGGTA 1441 ATTTGGGCTC ATAAGGCACT TATCGCTGAG GTGTCAATCG TCGGAGCCGC TGAGCAATAA 1501 CTAGCATAAC CCCTTGGGGC CTCTAAACGG GTCTTGAGGG GTTTTTTGCA TGGTCATAGC 1561 TGTTTCCTGA GAGCTTGGCA GGTGATGACA CACATTAACA AATTTCGTGA GGAGTCTCCA 1621 GAAGAATGCC ATTAATTTCC ATAGGCTCCG CCCCCCTGAC GAGCATCACA AAAATCGACG 1681 CTCAAGTCAG AGGTGGCGAA ACCCGACAGG ACTATAAAGA TACCAGGCGT TTCCCCCTGG 1741 AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC CCTGCCGCTT ACCGGATACC TGTCCGCCTT 1801 TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA TAGCTCACGC TGTAGGTATC TCAGTTCGGT 1861 GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT GCACGAACCC CCCGTTCAGC CCGACCGCTG 1921 CGCCTTATCC GGTAACTATC GTCTTGAGTC CAACCCGGTA AGACACGACT TATCGCCACT 1981 GGCAGCAGCC ACTGGTAACA GGATTAGCAG AGCGAGGTAT GTAGGCGGTG CTACAGAGTT 2041 CTTGAAGTGG TGGCCTAACT ACGGCTACAC TAGAAGAACA GTATTTGGTA TCTGCGCTCT 2101 GCTGAAGCCA GTTACCTTCG GAAAAAGAGT TGGTAGCTCT TGATCCGGCA AACAAACCAC 2161 CGCTGGTAGC GGTGGTTTTT TTGTTTGCAA GCAGCAGATT ACGCGCAGAA AAAAAGGATC 2221 TCAAGAAGGC CTACTATTAG CAACAACGAT CCTTTGATCT TTTCTACGGG GTCTGACGCT 2281 CAGTGGAACG AAAACTCACG TTAAGGGATT TTGGTCATGA GATTATCAAA AAGGATCTTC 2341 ACCTAGATCC TTTTAAATTA AAAATGAAGT TTTAAATCAA TCTAAAGTAT ATATGAGTAA 2401 ACTTGGTCTG ACAGTTACCA ATGCTTAATC AGTGAGGCAC CTATCTCAGC GATCTGTCTA 2461 TTTCGTTCAT CCATAGTTGC CTGACTCCCC GTCGTGTAGA TAACTACGAT ACGGGAGGGC 2521 TTACCATCTG GCCCCAGTGC TGCAATGATA CCGCGAGAAC CACGCTCACC GGCTCCAGAT 2581 TTATCAGCAA TAAACCAGCC AGCCGGAAGG GCCGAGCGCA GAAGTGGTCC TGCAACTTTA 2641 TCCGCCTCCA TCCAGTCTAT TAATTGTTGC CGGGAAGCTA GAGTAAGTAG TTCGCCAGTT 2701 AATAGTTTGC GCAACGTTGT TGCCATTGCT ACAGGCATCG TGGTGTCACG CTCGTCGTTT 2761 GGTATGGCTT CATTCAGCTC CGGTTCCCAA CGATCAAGGC GAGTTACATG ATCCCCCATG 2821 TTGTGCAAAA AAGCGGTTAG CTCCTTCGGT CCTCCGATCG TTGTCAGAAG TAAGTTGGCC 2881 GCAGTGTTAT CACTCATGGT TATGGCAGCA CTGCATAATT CTCTTACTGT CATGCCATCC 2941 GTAAGATGCT TTTCTGTGAC TGGTGAGTAC TCAACCAAGT CATTCTGAGA ATAGTGTATG 3001 CGGCGACCGA GTTGCTCTTG CCCGGCGTCA ATACGGGATA ATACCGCGCC ACATAGCAGA 3061 ACTTTAAAAG TGCTCATCAT TGGAAAACGT TCTTCGGGGC GAAAACTCTC AAGGATCTTA 3121 CCGCTGTTGA GATCCAGTTC GATGTAACCC ACTCGTGCAC CCAACTGATC TTCAGCATCT 3181 TTTACTTTCA CCAGCGTTTC TGGGTGAGCA AAAACAGGAA GGCAAAATGC CGCAAAAAAG 3241 GGAATAAGGG CGACACGGAA ATGTTGAATA CTCATACTCT TCCTTTTTCA ATATTATTGA 3301 AGCATTTATC AGGGTTATTG TCTCATGAGC GGATACATAT TTGAATGTAT TTAGAAAAAT 3361 AAACAAATAG GGGTTCCGCG CACCTGCACC AGTCAGTAAA ACGACGGCCA GTAGTCAAAA 3421 GCCTCCGACC GGAGGCTTTT GACTTGGTTC AGGTGGAGTG GGAGAACGAT GATGCTCACT 3481 CTCGGGTCTC A //