LOCUS BBF10K_003437 3026 bp DNA circular 17-Jun-2025 ACCESSION BBF10K_003437 VERSION BBF10K_003437.1 FEATURES Location/Qualifiers CDS 5..906 /label=CD_gp32 CDS complement(1949..2808) /label=AmpR promoter complement(2810..2913) /label=AmpR promoter primer_bind 2932..2948 /label=M13 fwd primer_bind complement(1087..1102) /label=M13 rev rep_origin complement(1170..1757) /label=ColE1 primer_bind 1004..1022 /label=SLiCE-for primer_bind complement(2976..2993) /label=SLiCE-rev misc_feature 1761..1950 /label=spacer CDS 1121..1171 /label=spacer terminator 1037..1084 /label=T7 terminator terminator 2949..2980 /label=tonB terminator misc_feature 999..2999 /label=pOpen_v3 backbone misc_feature 3020..3025 /label=BsaI_site misc_feature complement(912..917) /label=BsaI_site misc_feature 1..4 /label=AATG misc_feature 907..910 /label=AGGT ORIGIN 1 AATGTTCAAA CGTAAAAGCA CCGCAGAACT GGCAGCACAG ATGGCAAAAC TGAATGGTAA 61 TAAAGGTTTT AGCAGCGAGG ATAAAGGTGA GTGGAAACTG AAACTGGATA ATGCCGGTAA 121 TGGTCAGGCA GTTATTCGTT TTCTGCCGAG CAAAAATGAT GAACAGGCAC CGTTTGCAAT 181 TCTGGTGAAT CATGGTTTCA AAAAGAACGG CAAATGGTAC ATTGAAACCT GTAGCAGCAC 241 CCATGGTGAT TATGATAGCT GTCCGGTTTG TCAGTATATC AGCAAAAACG ATCTGTACAA 301 CACCGATAAC AAAGAATACA GCCTGGTTAA ACGCAAAACC AGCTATTGGG CAAATATTCT 361 GGTTGTTAAA GATCCGGCAG CACCGGAAAA TGAAGGTAAA GTTTTCAAAT ATCGCTTTGG 421 CAAGAAGATC TGGGATAAAA TCAATGCCAT GATTGCCGTT GATGTGGAAA TGGGTGAAAC 481 ACCGGTTGAT GTTACCTGTC CGTGGGAAGG TGCAAATTTT GTTCTGAAAG TTAAACAGGT 541 GAGCGGCTTC AGCAATTATG ATGAAAGCAA ATTTCTGAAC CAGAGCGCCA TTCCGAATAT 601 CGATGATGAA TCATTTCAGA AAGAACTGTT CGAGCAGATG GTTGATCTGA GCGAAATGAC 661 CAGCAAAGAC AAATTCAAAA GCTTCGAAGA ACTGAACACG AAATTTGGTC AGGTTATGGG 721 CACCGCAGTT ATGGGTGGTG CAGCAGCAAC CGCAGCAAAA AAAGCAGATA AAGTTGCAGA 781 TGATCTGGAT GCCTTTAACG TGGATGATTT TAACACCAAA ACCGAGGATG ATTTCATGAG 841 CAGCAGCAGC GGTAGCAGCA GCTCAGCAGA TGATACCGAT CTGGACGATC TGCTGAATGA 901 TCTGGGAGGT CGAGACCAGT ATGTCCCCTA AGCTCTGGTA TGTGTGATCG GTACTGATAT 961 AGGGATAACA GGGTAATAAA TGTCCGGCCG TAGGGTACCG CTGAGGTGTC AATCGTCGGA 1021 GCCGCTGAGC AATAACTAGC ATAACCCCTT GGGGCCTCTA AACGGGTCTT GAGGGGTTTT 1081 TTGCATGGTC ATAGCTGTTT CCTGAGAGCT TGGCAGGTGA TGACACACAT TAACAAATTT 1141 CGTGAGGAGT CTCCAGAAGA ATGCCATTAA TTTCCATAGG CTCCGCCCCC CTGACGAGCA 1201 TCACAAAAAT CGACGCTCAA GTCAGAGGTG GCGAAACCCG ACAGGACTAT AAAGATACCA 1261 GGCGTTTCCC CCTGGAAGCT CCCTCGTGCG CTCTCCTGTT CCGACCCTGC CGCTTACCGG 1321 ATACCTGTCC GCCTTTCTCC CTTCGGGAAG CGTGGCGCTT TCTCATAGCT CACGCTGTAG 1381 GTATCTCAGT TCGGTGTAGG TCGTTCGCTC CAAGCTGGGC TGTGTGCACG AACCCCCCGT 1441 TCAGCCCGAC CGCTGCGCCT TATCCGGTAA CTATCGTCTT GAGTCCAACC CGGTAAGACA 1501 CGACTTATCG CCACTGGCAG CAGCCACTGG TAACAGGATT AGCAGAGCGA GGTATGTAGG 1561 CGGTGCTACA GAGTTCTTGA AGTGGTGGCC TAACTACGGC TACACTAGAA GAACAGTATT 1621 TGGTATCTGC GCTCTGCTGA AGCCAGTTAC CTTCGGAAAA AGAGTTGGTA GCTCTTGATC 1681 CGGCAAACAA ACCACCGCTG GTAGCGGTGG TTTTTTTGTT TGCAAGCAGC AGATTACGCG 1741 CAGAAAAAAA GGATCTCAAG AAGGCCTACT ATTAGCAACA ACGATCCTTT GATCTTTTCT 1801 ACGGGGTCTG ACGCTCAGTG GAACGAAAAC TCACGTTAAG GGATTTTGGT CATGAGATTA 1861 TCAAAAAGGA TCTTCACCTA GATCCTTTTA AATTAAAAAT GAAGTTTTAA ATCAATCTAA 1921 AGTATATATG AGTAAACTTG GTCTGACAGT TACCAATGCT TAATCAGTGA GGCACCTATC 1981 TCAGCGATCT GTCTATTTCG TTCATCCATA GTTGCCTGAC TCCCCGTCGT GTAGATAACT 2041 ACGATACGGG AGGGCTTACC ATCTGGCCCC AGTGCTGCAA TGATACCGCG AGAACCACGC 2101 TCACCGGCTC CAGATTTATC AGCAATAAAC CAGCCAGCCG GAAGGGCCGA GCGCAGAAGT 2161 GGTCCTGCAA CTTTATCCGC CTCCATCCAG TCTATTAATT GTTGCCGGGA AGCTAGAGTA 2221 AGTAGTTCGC CAGTTAATAG TTTGCGCAAC GTTGTTGCCA TTGCTACAGG CATCGTGGTG 2281 TCACGCTCGT CGTTTGGTAT GGCTTCATTC AGCTCCGGTT CCCAACGATC AAGGCGAGTT 2341 ACATGATCCC CCATGTTGTG CAAAAAAGCG GTTAGCTCCT TCGGTCCTCC GATCGTTGTC 2401 AGAAGTAAGT TGGCCGCAGT GTTATCACTC ATGGTTATGG CAGCACTGCA TAATTCTCTT 2461 ACTGTCATGC CATCCGTAAG ATGCTTTTCT GTGACTGGTG AGTACTCAAC CAAGTCATTC 2521 TGAGAATAGT GTATGCGGCG ACCGAGTTGC TCTTGCCCGG CGTCAATACG GGATAATACC 2581 GCGCCACATA GCAGAACTTT AAAAGTGCTC ATCATTGGAA AACGTTCTTC GGGGCGAAAA 2641 CTCTCAAGGA TCTTACCGCT GTTGAGATCC AGTTCGATGT AACCCACTCG TGCACCCAAC 2701 TGATCTTCAG CATCTTTTAC TTTCACCAGC GTTTCTGGGT GAGCAAAAAC AGGAAGGCAA 2761 AATGCCGCAA AAAAGGGAAT AAGGGCGACA CGGAAATGTT GAATACTCAT ACTCTTCCTT 2821 TTTCAATATT ATTGAAGCAT TTATCAGGGT TATTGTCTCA TGAGCGGATA CATATTTGAA 2881 TGTATTTAGA AAAATAAACA AATAGGGGTT CCGCGCACCT GCACCAGTCA GTAAAACGAC 2941 GGCCAGTAGT CAAAAGCCTC CGACCGGAGG CTTTTGACTT GGTTCAGGTG GAGTGGGAGA 3001 ACGATGATGC TCACTCTCGG GTCTCA //