LOCUS BBF10K_003438 3878 bp DNA circular 29-Apr-2025 ACCESSION BBF10K_003438 VERSION BBF10K_003438.1 FEATURES Location/Qualifiers CDS 5..1758 /label=CD_BsuLF CDS complement(2801..3660) /label=AmpR promoter complement(3662..3765) /label=AmpR promoter primer_bind 3784..3800 /label=M13 fwd primer_bind complement(1939..1954) /label=M13 rev rep_origin complement(2022..2609) /label=ColE1 primer_bind 1856..1874 /label=SLiCE-for primer_bind complement(3828..3845) /label=SLiCE-rev misc_feature 2613..2802 /label=spacer CDS 1973..2023 /label=spacer terminator 1889..1936 /label=T7 terminator terminator 3801..3832 /label=tonB terminator misc_feature 1851..3851 /label=pOpen_v3 backbone misc_feature 3872..3877 /label=BsaI_site misc_feature complement(1764..1769) /label=BsaI_site misc_feature 1..4 /label=AATG misc_feature 1759..1762 /label=AGGT ORIGIN 1 AATGGATCAG AGCCTGGAAG ATATTAATGT TAAAACCGTT ACCGATGTGA CCAGCGATAT 61 TCTGGTTAGC CCGAGCGCAT TTGTTGTTGA GCAGATTGGT GATAACTATC ACGAAGAACC 121 GATTCTGGGT TTTAGCATTG TTAATGAAAC CGGTGCCTAT TTCATCCCGA AAGATATTGC 181 AGTTGAAAGC GAAGTGTTCA AAGAATGGGT TGAAAACGAC GAGCAGAAAA AATGGGTGTT 241 TGATAGCAAA CGTGCAGTTG TTGCACTGCG TTGGCAGGGT ATTGAACTGA AAGGTGCAGA 301 ATTTGATACC CTGCTGGCAG CCTATATTAT CAATCCGGGT AATAGCTATG ATGATGTTGC 361 AAGCGTTGCC AAAGATTATG GTCTGCATAT TGTTAGCAGT GATGAAAGCG TTTATGGCAA 421 AGGTGCAAAA CGTGCCGTTC CGAGCGAAGA TGTTCTGAGC GAACATCTGG GTCGTAAAGC 481 ACTGGCAATT CAGAGTCTGC GTGAAAAACT GGTTCAAGAA CTGGAAAATA ACGATCAGCT 541 GGAACTGTTT GAAGAATTAG AAATGCCGCT GGCACTGATT CTGGGCGAAA TGGAAAGCAC 601 CGGTGTTAAA GTTGATGTTG ATCGTCTGAA ACGTATGGGT GAAGAACTGG GTGCCAAACT 661 GAAAGAATAT GAAGAAAAAA TCCACGAGAT TGCCGGTGAA CCGTTTAATA TCAATAGCCC 721 GAAACAGCTG GGTGTGATCC TGTTTGAAAA AATTGGTCTG CCGGTTGTGA AAAAAACCAA 781 AACCGGTTAT AGCACCAGCG CAGATGTGCT GGAAAAATTA GCAGATAAAC ATGACATCGT 841 GGACTACATT CTGCAGTATC GTCAGATTGG TAAACTGCAG AGCACCTATA TTGAAGGTCT 901 GCTGAAAGTT ACCCGTCCGG ATAGCCATAA AGTTCATACC CGTTTTAATC AGGCACTGAC 961 CCAGACCGGT CGTCTGAGCA GCACCGATCC GAATCTGCAG AATATTCCGA TTCGTCTGGA 1021 AGAAGGTCGT AAAATTCGTC AGGCATTTGT GCCGAGCGAA AAAGATTGGC TGATTTTTGC 1081 AGCAGATTAT AGCCAGATTG AACTGCGTGT TCTGGCACAT ATTAGCAAAG ATGAAAATCT 1141 GATCGAAGCC TTCACCAACG ATATGGATAT TCATACCAAA ACAGCCATGG ATGTGTTTCA 1201 TGTTGCGAAA GATGAAGTTA CCAGCGCAAT GCGTCGTCAG GCCAAAGCAG TTAATTTTGG 1261 TATTGTGTAT GGCATCAGCG ATTATGGCCT GAGCCAGAAT CTGGGTATTA CCCGTAAAGA 1321 AGCAGGCGCA TTTATTGATC GCTATCTGGA AAGTTTTCAG GGCGTTAAAG CATACATGGA 1381 AGATAGCGTT CAAGAGGCAA AACAGAAAGG TTATGTTACC ACACTGATGC ATCGTCGTCG 1441 TTATATTCCG GAACTGACCA GCCGTAATTT CAATATTCGT AGCTTTGCAG AACGTACCGC 1501 AATGAATACC CCGATTCAGG GTAGCGCAGC AGATATCATC AAAAAAGCAA TGATTGACAT 1561 GGCAGCGAAG CTGAAAGAAA AACAGCTGAA AGCACGTCTG CTGCTGCAAG TGCATGATGA 1621 ACTGATCTTT GAAGCACCGA AAGAAGAAAT CGAGATCCTG GAAAAGCTGG TTCCGGAAGT 1681 TATGGAACAT GCACTGGCAC TGGATGTTCC GCTGAAAGTG GATTTTGCAA GCGGTCCGAG 1741 CTGGTATGAT GCAAAAGGAG GTCGAGACCA GGTATATTTC TCCCCTCGGG CGTCTTGAAG 1801 CCCACGACCC CTAGGGATAA CAGGGTAATG TGAAGGGCAT CCCCTTTCAC CGCTGAGGTG 1861 TCAATCGTCG GAGCCGCTGA GCAATAACTA GCATAACCCC TTGGGGCCTC TAAACGGGTC 1921 TTGAGGGGTT TTTTGCATGG TCATAGCTGT TTCCTGAGAG CTTGGCAGGT GATGACACAC 1981 ATTAACAAAT TTCGTGAGGA GTCTCCAGAA GAATGCCATT AATTTCCATA GGCTCCGCCC 2041 CCCTGACGAG CATCACAAAA ATCGACGCTC AAGTCAGAGG TGGCGAAACC CGACAGGACT 2101 ATAAAGATAC CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG TTCCGACCCT 2161 GCCGCTTACC GGATACCTGT CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC TTTCTCATAG 2221 CTCACGCTGT AGGTATCTCA GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG GCTGTGTGCA 2281 CGAACCCCCC GTTCAGCCCG ACCGCTGCGC CTTATCCGGT AACTATCGTC TTGAGTCCAA 2341 CCCGGTAAGA CACGACTTAT CGCCACTGGC AGCAGCCACT GGTAACAGGA TTAGCAGAGC 2401 GAGGTATGTA GGCGGTGCTA CAGAGTTCTT GAAGTGGTGG CCTAACTACG GCTACACTAG 2461 AAGAACAGTA TTTGGTATCT GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA AAAGAGTTGG 2521 TAGCTCTTGA TCCGGCAAAC AAACCACCGC TGGTAGCGGT GGTTTTTTTG TTTGCAAGCA 2581 GCAGATTACG CGCAGAAAAA AAGGATCTCA AGAAGGCCTA CTATTAGCAA CAACGATCCT 2641 TTGATCTTTT CTACGGGGTC TGACGCTCAG TGGAACGAAA ACTCACGTTA AGGGATTTTG 2701 GTCATGAGAT TATCAAAAAG GATCTTCACC TAGATCCTTT TAAATTAAAA ATGAAGTTTT 2761 AAATCAATCT AAAGTATATA TGAGTAAACT TGGTCTGACA GTTACCAATG CTTAATCAGT 2821 GAGGCACCTA TCTCAGCGAT CTGTCTATTT CGTTCATCCA TAGTTGCCTG ACTCCCCGTC 2881 GTGTAGATAA CTACGATACG GGAGGGCTTA CCATCTGGCC CCAGTGCTGC AATGATACCG 2941 CGAGAACCAC GCTCACCGGC TCCAGATTTA TCAGCAATAA ACCAGCCAGC CGGAAGGGCC 3001 GAGCGCAGAA GTGGTCCTGC AACTTTATCC GCCTCCATCC AGTCTATTAA TTGTTGCCGG 3061 GAAGCTAGAG TAAGTAGTTC GCCAGTTAAT AGTTTGCGCA ACGTTGTTGC CATTGCTACA 3121 GGCATCGTGG TGTCACGCTC GTCGTTTGGT ATGGCTTCAT TCAGCTCCGG TTCCCAACGA 3181 TCAAGGCGAG TTACATGATC CCCCATGTTG TGCAAAAAAG CGGTTAGCTC CTTCGGTCCT 3241 CCGATCGTTG TCAGAAGTAA GTTGGCCGCA GTGTTATCAC TCATGGTTAT GGCAGCACTG 3301 CATAATTCTC TTACTGTCAT GCCATCCGTA AGATGCTTTT CTGTGACTGG TGAGTACTCA 3361 ACCAAGTCAT TCTGAGAATA GTGTATGCGG CGACCGAGTT GCTCTTGCCC GGCGTCAATA 3421 CGGGATAATA CCGCGCCACA TAGCAGAACT TTAAAAGTGC TCATCATTGG AAAACGTTCT 3481 TCGGGGCGAA AACTCTCAAG GATCTTACCG CTGTTGAGAT CCAGTTCGAT GTAACCCACT 3541 CGTGCACCCA ACTGATCTTC AGCATCTTTT ACTTTCACCA GCGTTTCTGG GTGAGCAAAA 3601 ACAGGAAGGC AAAATGCCGC AAAAAAGGGA ATAAGGGCGA CACGGAAATG TTGAATACTC 3661 ATACTCTTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG GTTATTGTCT CATGAGCGGA 3721 TACATATTTG AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC CTGCACCAGT 3781 CAGTAAAACG ACGGCCAGTA GTCAAAAGCC TCCGACCGGA GGCTTTTGAC TTGGTTCAGG 3841 TGGAGTGGGA GAACGATGAT GCTCACTCTC GGGTCTCA //