LOCUS       BBF10K_003439           2576 bp    DNA     circular     29-Apr-2025
ACCESSION   BBF10K_003439
VERSION     BBF10K_003439.1  
FEATURES             Location/Qualifiers
     CDS             5..456
                     /label=CD_UvsY
     CDS             complement(1499..2358)
                     /label=AmpR
     promoter        complement(2360..2463)
                     /label=AmpR promoter
     primer_bind     2482..2498
                     /label=M13 fwd
     primer_bind     complement(637..652)
                     /label=M13 rev
     rep_origin      complement(720..1307)
                     /label=ColE1
     primer_bind     554..572
                     /label=SLiCE-for
     primer_bind     complement(2526..2543)
                     /label=SLiCE-rev
     misc_feature    1311..1500
                     /label=spacer
     CDS             671..721
                     /label=spacer
     terminator      587..634
                     /label=T7 terminator
     terminator      2499..2530
                     /label=tonB terminator
     misc_feature    549..2549
                     /label=pOpen_v3 backbone
     misc_feature    2570..2575
                     /label=BsaI_site
     misc_feature    complement(462..467)
                     /label=BsaI_site
     misc_feature    1..4
                     /label=AATG
     misc_feature    457..460
                     /label=AGGT
ORIGIN
        1 AATGAAAATC GAAGAAGGTA AAGGTCACCA TCACCATCAC CACATGCGTC TGGAAGATCT
       61 GCAAGAGGAA CTGAAAAAAG ATGTGTTTAT CGACAGCACC AAACTGCAGT ATGAAGCAGC
      121 AAATAATGTG ATGCTGTATA GCAAATGGCT GAACAAACAT AGCAGCATCA AAAAAGAAAT
      181 GCTGCGTATT GAGGCCCAGA AAAAAGTTGC ACTGAAAGCA CGTCTGGATT ATTACAGCGG
      241 TCGTGGTGAT GGTGATGAAT TTAGCATGGA TCGTTATGAG AAAAGCGAGA TGAAAACCGT
      301 TCTGAGCGCA GATAAAGATG TTCTGAAAGT TGATACCAGC CTGCAGTATT GGGGTATTCT
      361 GCTGGATTTT TGTAGCGGTG CACTGGATGC AATTAAAAGC CGTGGTTTTG CCATTAAACA
      421 TATCCAGGAC ATGCGTGCAT TTGAAGCCGG TAAAGGAGGT CGAGACCACT GTATCATGGT
      481 TCCCTCGGGA GCTTTTTCGC GCAAAGCAGT AGGGATAACA GGGTAATTAG AAGGAAGGGA
      541 ATTTACAGCG CTGAGGTGTC AATCGTCGGA GCCGCTGAGC AATAACTAGC ATAACCCCTT
      601 GGGGCCTCTA AACGGGTCTT GAGGGGTTTT TTGCATGGTC ATAGCTGTTT CCTGAGAGCT
      661 TGGCAGGTGA TGACACACAT TAACAAATTT CGTGAGGAGT CTCCAGAAGA ATGCCATTAA
      721 TTTCCATAGG CTCCGCCCCC CTGACGAGCA TCACAAAAAT CGACGCTCAA GTCAGAGGTG
      781 GCGAAACCCG ACAGGACTAT AAAGATACCA GGCGTTTCCC CCTGGAAGCT CCCTCGTGCG
      841 CTCTCCTGTT CCGACCCTGC CGCTTACCGG ATACCTGTCC GCCTTTCTCC CTTCGGGAAG
      901 CGTGGCGCTT TCTCATAGCT CACGCTGTAG GTATCTCAGT TCGGTGTAGG TCGTTCGCTC
      961 CAAGCTGGGC TGTGTGCACG AACCCCCCGT TCAGCCCGAC CGCTGCGCCT TATCCGGTAA
     1021 CTATCGTCTT GAGTCCAACC CGGTAAGACA CGACTTATCG CCACTGGCAG CAGCCACTGG
     1081 TAACAGGATT AGCAGAGCGA GGTATGTAGG CGGTGCTACA GAGTTCTTGA AGTGGTGGCC
     1141 TAACTACGGC TACACTAGAA GAACAGTATT TGGTATCTGC GCTCTGCTGA AGCCAGTTAC
     1201 CTTCGGAAAA AGAGTTGGTA GCTCTTGATC CGGCAAACAA ACCACCGCTG GTAGCGGTGG
     1261 TTTTTTTGTT TGCAAGCAGC AGATTACGCG CAGAAAAAAA GGATCTCAAG AAGGCCTACT
     1321 ATTAGCAACA ACGATCCTTT GATCTTTTCT ACGGGGTCTG ACGCTCAGTG GAACGAAAAC
     1381 TCACGTTAAG GGATTTTGGT CATGAGATTA TCAAAAAGGA TCTTCACCTA GATCCTTTTA
     1441 AATTAAAAAT GAAGTTTTAA ATCAATCTAA AGTATATATG AGTAAACTTG GTCTGACAGT
     1501 TACCAATGCT TAATCAGTGA GGCACCTATC TCAGCGATCT GTCTATTTCG TTCATCCATA
     1561 GTTGCCTGAC TCCCCGTCGT GTAGATAACT ACGATACGGG AGGGCTTACC ATCTGGCCCC
     1621 AGTGCTGCAA TGATACCGCG AGAACCACGC TCACCGGCTC CAGATTTATC AGCAATAAAC
     1681 CAGCCAGCCG GAAGGGCCGA GCGCAGAAGT GGTCCTGCAA CTTTATCCGC CTCCATCCAG
     1741 TCTATTAATT GTTGCCGGGA AGCTAGAGTA AGTAGTTCGC CAGTTAATAG TTTGCGCAAC
     1801 GTTGTTGCCA TTGCTACAGG CATCGTGGTG TCACGCTCGT CGTTTGGTAT GGCTTCATTC
     1861 AGCTCCGGTT CCCAACGATC AAGGCGAGTT ACATGATCCC CCATGTTGTG CAAAAAAGCG
     1921 GTTAGCTCCT TCGGTCCTCC GATCGTTGTC AGAAGTAAGT TGGCCGCAGT GTTATCACTC
     1981 ATGGTTATGG CAGCACTGCA TAATTCTCTT ACTGTCATGC CATCCGTAAG ATGCTTTTCT
     2041 GTGACTGGTG AGTACTCAAC CAAGTCATTC TGAGAATAGT GTATGCGGCG ACCGAGTTGC
     2101 TCTTGCCCGG CGTCAATACG GGATAATACC GCGCCACATA GCAGAACTTT AAAAGTGCTC
     2161 ATCATTGGAA AACGTTCTTC GGGGCGAAAA CTCTCAAGGA TCTTACCGCT GTTGAGATCC
     2221 AGTTCGATGT AACCCACTCG TGCACCCAAC TGATCTTCAG CATCTTTTAC TTTCACCAGC
     2281 GTTTCTGGGT GAGCAAAAAC AGGAAGGCAA AATGCCGCAA AAAAGGGAAT AAGGGCGACA
     2341 CGGAAATGTT GAATACTCAT ACTCTTCCTT TTTCAATATT ATTGAAGCAT TTATCAGGGT
     2401 TATTGTCTCA TGAGCGGATA CATATTTGAA TGTATTTAGA AAAATAAACA AATAGGGGTT
     2461 CCGCGCACCT GCACCAGTCA GTAAAACGAC GGCCAGTAGT CAAAAGCCTC CGACCGGAGG
     2521 CTTTTGACTT GGTTCAGGTG GAGTGGGAGA ACGATGATGC TCACTCTCGG GTCTCA
//