LOCUS BBF10K_003440 3296 bp DNA circular 29-Apr-2025 ACCESSION BBF10K_003440 VERSION BBF10K_003440.1 FEATURES Location/Qualifiers CDS 5..1176 /label=CD_UvsX CDS complement(2219..3078) /label=AmpR promoter complement(3080..3183) /label=AmpR promoter primer_bind 3202..3218 /label=M13 fwd primer_bind complement(1357..1372) /label=M13 rev rep_origin complement(1440..2027) /label=ColE1 primer_bind 1274..1292 /label=SLiCE-for primer_bind complement(3246..3263) /label=SLiCE-rev misc_feature 2031..2220 /label=spacer CDS 1391..1441 /label=spacer terminator 1307..1354 /label=T7 terminator terminator 3219..3250 /label=tonB terminator misc_feature 1269..3269 /label=pOpen_v3 backbone misc_feature 3290..3295 /label=BsaI_site misc_feature complement(1182..1187) /label=BsaI_site misc_feature 1..4 /label=AATG misc_feature 1177..1180 /label=AGGT ORIGIN 1 AATGAGCGAT CTGAAAAGCC GTCTGATTAA AGCAAGCACC AGCAAACTGA CCGCAGAACT 61 GACAGCAAGC AAATTTTTCA ACGAAAAAGA TGTGGTGCGC ACGAAAATTC CGATGATGAA 121 TATTGCACTG AGCGGTGAAA TTACCGGTGG TATGCAGAGC GGTCTGCTGA TTCTGGCTGG 181 TCCGAGCAAA AGCTTTAAAA GCAATTTTGG TCTGACCATG GTGAGCAGCT ATATGCGTCA 241 GTATCCGGAT GCAGTTTGTC TGTTTTATGA TAGCGAATTT GGTATCACAC CGGCATATCT 301 GCGTAGCATG GGTGTTGATC CGGAACGTGT TATTCATACA CCGGTTCAGA GCCTGGAACA 361 GCTGCGTATT GATATGGTTA ATCAGCTGGA TGCAATTGAA CGTGGTGAAA AAGTGGTGGT 421 GTTTATTGAT AGCCTGGGTA ATCTGGCAAG CAAAAAAGAA ACCGAAGATG CCCTGAATGA 481 AAAAGTTGTT AGCGATATGA CCCGTGCCAA AACCATGAAA AGCCTGTTTC GTATTGTTAC 541 CCCGTATTTC AGCACCAAAA ACATTCCGTG TATTGCCATC AACCATACCT ATGAAACCCA 601 AGAGATGTTT AGCAAAACCG TTATGGGTGG TGGCACCGGT CCGATGTATA GCGCAGATAC 661 CGTTTTTATC ATTGGCAAAC GCCAGATTAA AGATGGTAGC GATCTGCAGG GTTATCAGTT 721 TGTTCTGAAT GTTGAAAAAA GCCGCACCGT GAAAGAGAAA AGCAAATTCT TTATCGACGT 781 GAAATTCGAT GGTGGCATTG ATCCGTATAG TGGTCTGCTG GATATGGCAC TGGAACTGGG 841 TTTTGTTGTT AAACCGAAAA ATGGTTGGTA TGCCCGTGAA TTTCTGGATG AAGAAACCGG 901 TGAAATGATC CGTGAAGAAA AAAGCTGGCG TGCAAAAGAT ACCAATTGTA CCACCTTTTG 961 GGGTCCGCTG TTCAAACATC AGCCGTTTCG TGATGCCATT AAACGTGCAT ATCAGCTGGG 1021 TGCCATCGAT AGCAATGAAA TTGTTGAAGC CGAAGTGGAT GAACTGATCA ATAGCAAAGT 1081 GGAAAAATTC AAGAGTCCGG AATCCAAAAG CAAAAGCGCA GCAGATCTGG AAACCGATCT 1141 GGAACAACTG AGCGATATGG AAGAATTTAA CGAAGGAGGT CGAGACCAGT GCTATATCTT 1201 CCTCCCGGGC CGCAACAATG TCATTCGTAT AGGGATAACA GGGTAATTAG TAGGCGCAGC 1261 ACCATACTCG CTGAGGTGTC AATCGTCGGA GCCGCTGAGC AATAACTAGC ATAACCCCTT 1321 GGGGCCTCTA AACGGGTCTT GAGGGGTTTT TTGCATGGTC ATAGCTGTTT CCTGAGAGCT 1381 TGGCAGGTGA TGACACACAT TAACAAATTT CGTGAGGAGT CTCCAGAAGA ATGCCATTAA 1441 TTTCCATAGG CTCCGCCCCC CTGACGAGCA TCACAAAAAT CGACGCTCAA GTCAGAGGTG 1501 GCGAAACCCG ACAGGACTAT AAAGATACCA GGCGTTTCCC CCTGGAAGCT CCCTCGTGCG 1561 CTCTCCTGTT CCGACCCTGC CGCTTACCGG ATACCTGTCC GCCTTTCTCC CTTCGGGAAG 1621 CGTGGCGCTT TCTCATAGCT CACGCTGTAG GTATCTCAGT TCGGTGTAGG TCGTTCGCTC 1681 CAAGCTGGGC TGTGTGCACG AACCCCCCGT TCAGCCCGAC CGCTGCGCCT TATCCGGTAA 1741 CTATCGTCTT GAGTCCAACC CGGTAAGACA CGACTTATCG CCACTGGCAG CAGCCACTGG 1801 TAACAGGATT AGCAGAGCGA GGTATGTAGG CGGTGCTACA GAGTTCTTGA AGTGGTGGCC 1861 TAACTACGGC TACACTAGAA GAACAGTATT TGGTATCTGC GCTCTGCTGA AGCCAGTTAC 1921 CTTCGGAAAA AGAGTTGGTA GCTCTTGATC CGGCAAACAA ACCACCGCTG GTAGCGGTGG 1981 TTTTTTTGTT TGCAAGCAGC AGATTACGCG CAGAAAAAAA GGATCTCAAG AAGGCCTACT 2041 ATTAGCAACA ACGATCCTTT GATCTTTTCT ACGGGGTCTG ACGCTCAGTG GAACGAAAAC 2101 TCACGTTAAG GGATTTTGGT CATGAGATTA TCAAAAAGGA TCTTCACCTA GATCCTTTTA 2161 AATTAAAAAT GAAGTTTTAA ATCAATCTAA AGTATATATG AGTAAACTTG GTCTGACAGT 2221 TACCAATGCT TAATCAGTGA GGCACCTATC TCAGCGATCT GTCTATTTCG TTCATCCATA 2281 GTTGCCTGAC TCCCCGTCGT GTAGATAACT ACGATACGGG AGGGCTTACC ATCTGGCCCC 2341 AGTGCTGCAA TGATACCGCG AGAACCACGC TCACCGGCTC CAGATTTATC AGCAATAAAC 2401 CAGCCAGCCG GAAGGGCCGA GCGCAGAAGT GGTCCTGCAA CTTTATCCGC CTCCATCCAG 2461 TCTATTAATT GTTGCCGGGA AGCTAGAGTA AGTAGTTCGC CAGTTAATAG TTTGCGCAAC 2521 GTTGTTGCCA TTGCTACAGG CATCGTGGTG TCACGCTCGT CGTTTGGTAT GGCTTCATTC 2581 AGCTCCGGTT CCCAACGATC AAGGCGAGTT ACATGATCCC CCATGTTGTG CAAAAAAGCG 2641 GTTAGCTCCT TCGGTCCTCC GATCGTTGTC AGAAGTAAGT TGGCCGCAGT GTTATCACTC 2701 ATGGTTATGG CAGCACTGCA TAATTCTCTT ACTGTCATGC CATCCGTAAG ATGCTTTTCT 2761 GTGACTGGTG AGTACTCAAC CAAGTCATTC TGAGAATAGT GTATGCGGCG ACCGAGTTGC 2821 TCTTGCCCGG CGTCAATACG GGATAATACC GCGCCACATA GCAGAACTTT AAAAGTGCTC 2881 ATCATTGGAA AACGTTCTTC GGGGCGAAAA CTCTCAAGGA TCTTACCGCT GTTGAGATCC 2941 AGTTCGATGT AACCCACTCG TGCACCCAAC TGATCTTCAG CATCTTTTAC TTTCACCAGC 3001 GTTTCTGGGT GAGCAAAAAC AGGAAGGCAA AATGCCGCAA AAAAGGGAAT AAGGGCGACA 3061 CGGAAATGTT GAATACTCAT ACTCTTCCTT TTTCAATATT ATTGAAGCAT TTATCAGGGT 3121 TATTGTCTCA TGAGCGGATA CATATTTGAA TGTATTTAGA AAAATAAACA AATAGGGGTT 3181 CCGCGCACCT GCACCAGTCA GTAAAACGAC GGCCAGTAGT CAAAAGCCTC CGACCGGAGG 3241 CTTTTGACTT GGTTCAGGTG GAGTGGGAGA ACGATGATGC TCACTCTCGG GTCTCA //