LOCUS BBF10K_003441 4776 bp DNA circular 29-Apr-2025 ACCESSION BBF10K_003441 VERSION BBF10K_003441.1 FEATURES Location/Qualifiers CDS 5..2656 /label=CE_T7RNApol_Stop CDS complement(3699..4558) /label=AmpR promoter complement(4560..4663) /label=AmpR promoter primer_bind 4682..4698 /label=M13 fwd primer_bind complement(2837..2852) /label=M13 rev rep_origin complement(2920..3507) /label=ColE1 primer_bind 2754..2772 /label=SLiCE-for primer_bind complement(4726..4743) /label=SLiCE-rev misc_feature 3511..3700 /label=spacer CDS 2871..2921 /label=spacer terminator 2787..2834 /label=T7 terminator terminator 4699..4730 /label=tonB terminator misc_feature 2749..4749 /label=pOpen_v3 backbone misc_feature 4770..4775 /label=BsaI_site misc_feature complement(2662..2667) /label=BsaI_site misc_feature 1..4 /label=AATG misc_feature 2657..2660 /label=GCTT ORIGIN 1 AATGAACACT ATCAACATTG CGAAGAACGA TTTCTCTGAC ATCGAACTCG CGGCAATCCC 61 GTTCAACACC CTGGCAGATC ACTACGGTGA ACGTCTGGCC CGTGAACAGC TGGCTCTGGA 121 ACACGAAAGC TATGAAATGG GTGAGGCGCG TTTCCGTAAG ATGTTCGAAC GTCAGTTAAA 181 AGCGGGTGAA GTTGCAGACA ACGCCGCGGC GAAGCCGCTG ATCACCACCC TGCTGCCGAA 241 AATGATCGCT CGCATCAACG ATTGGTTCGA AGAAGTGAAA GCGAAACGTG GCAAACGTCC 301 GACTGCCTTC CAGTTCCTGC AAGAAATTAA ACCGGAGGCA GTGGCGTATA TCACTATCAA 361 AACTACCCTG GCGTGCCTGA CCTCTGCGGA TAACACGACC GTTCAGGCGG TAGCGTCCGC 421 AATCGGTCGT GCGATCGAAG ATGAAGCCCG CTTCGGCCGT ATCCGTGACC TTGAAGCAAA 481 ACACTTTAAA AAAAACGTTG AAGAACAGCT GAACAAACGC GTTGGTCACG TATACAAAAA 541 GGCGTTTATG CAGGTTGTGG AAGCTGATAT GCTGTCCAAA GGCCTGCTGG GCGGCGAAGC 601 GTGGAGCTCT TGGCATAAAG AAGATTCTAT TCACGTGGGC GTTCGCTGCA TTGAAATGCT 661 GATCGAATCG ACTGGTATGG TTTCTCTGCA CCGTCAGAAC GCTGGTGTTG TTGGCCAGGA 721 TTCTGAAACT ATCGAACTGG CCCCGGAATA TGCGGAAGCG ATCGCTACTC GCGCAGGTGC 781 GCTGGCCGGT ATTTCCCCGA TGTTCCAGCC GTGCGTTGTA CCGCCTAAAC CGTGGACCGG 841 CATCACCGGC GGTGGTTATT GGGCTAACGG TCGCCGTCCG CTGGCACTCG TTCGTACCCA 901 CAGCAAAAAG GCACTGATGC GTTACGAAGA TGTGTACATG CCGGAGGTTT ACAAAGCCAT 961 CAACATTGCT CAGAATACCG CCTGGAAGAT TAATAAAAAA GTGCTCGCGG TGGCGAACGT 1021 TATTACCAAG TGGAAACACT GTCCGGTGGA AGATATCCCG GCTATCGAGC GCGAGGAACT 1081 GCCGATGAAA CCGGAAGATA TCGATATGAA CCCGGAAGCT CTGACCGCGT GGAAACGTGC 1141 TGCTGCTGCC GTATATCGCA AAGACAAGGC GCGTAAGTCT CGCCGTATCT CTCTGGAGTT 1201 TATGCTGGAA CAGGCGAACA AATTCGCCAA CCACAAAGCC ATCTGGTTCC CGTACAACAT 1261 GGATTGGCGT GGCCGTGTCT ATGCAGTTTC TATGTTCAAC CCGCAAGGGA ATGATATGAC 1321 CAAAGGCCTG CTGACTCTGG CAAAAGGTAA GCCGATCGGC AAAGAAGGTT ATTACTGGCT 1381 GAAAATCCAT GGCGCGAATT GCGCGGGTGT AGATAAGGTA CCGTTCCCGG AACGTATTAA 1441 GTTCATTGAA GAGAACCACG AGAATATCAT GGCATGCGCG AAAAGCCCGC TGGAAAACAC 1501 TTGGTGGGCT GAGCAGGACT CCCCGTTCTG CTTCCTGGCG TTCTGCTTCG AGTACGCAGG 1561 TGTTCAACAT CATGGCCTGA GCTACAACTG CTCCCTGCCT CTGGCATTCG ACGGTTCTTG 1621 CAGCGGTATC CAGCACTTTT CCGCTATGTT ACGTGATGAA GTAGGTGGCC GTGCTGTCAA 1681 TCTGCTGCCG TCCGAAACCG TTCAAGATAT CTATGGTATC GTAGCGAAAA AGGTTAACGA 1741 GATTCTGCAA GCAGATGCTA TCAACGGTAC CGACAACGAG GTAGTTACCG TGACCGATGA 1801 AAACACCGGT GAAATTTCCG AAAAAGTAAA ACTGGGGACC AAGGCACTGG CTGGCCAGTG 1861 GCTGGCCTAC GGCGTGACTC GCAGCGTCAC CAAACGTAGC GTGATGACGC TCGCTTATGG 1921 CAGCAAAGAG TTTGGCTTTC GCCAGCAAGT TCTGGAGGAT ACTATCCAGC CAGCCATTGA 1981 CTCGGGCAAG GGTCTGATGT TTACCCAGCC GAACCAGGCC GCTGGATATA TGGCAAAACT 2041 GATCTGGGAA TCTGTAAGCG TCACCGTTGT GGCAGCGGTT GAAGCTATGA ATTGGTTGAA 2101 AAGCGCCGCG AAACTGCTGG CAGCGGAAGT GAAAGATAAA AAAACTGGCG AAATCCTGCG 2161 TAAACGCTGC GCGGTTCACT GGGTCACTCC GGATGGCTTC CCAGTTTGGC AGGAATACAA 2221 AAAACCTATC CAGACCCGCC TGAATCTGAT GTTCCTGGGC CAATTCCGTC TGCAACCGAC 2281 CATCAACACC AACAAAGACA GCGAAATTGA CGCCCACAAA CAGGAGTCCG GCATTGCCCC 2341 TAACTTCGTT CACTCTCAGG ACGGCTCTCA TCTGCGCAAA ACTGTCGTTT GGGCTCATGA 2401 AAAGTACGGC ATCGAATCCT TTGCGCTGAT CCACGACTCC TTCGGTACCA TCCCGGCGGA 2461 CGCGGCCAAC CTGTTCAAAG CTGTTCGTGA AACTATGGTA GACACGTACG AAAGCTGCGA 2521 CGTTCTGGCA GACTTCTACG ATCAGTTCGC TGACCAGCTG CACGAAAGCC AGCTGGACAA 2581 GATGCCAGCT CTGCCGGCTA AAGGTAATCT GAACCTGCGC GACATTCTGG AATCTGACTT 2641 CGCTTTCGCC TAATAAGCTT CGAGACCACA CTCGATAGGT ACAACCGGCC TAGCTGACTC 2701 ATTAGGCTGT AGGGATAACA GGGTAATCGT GACTAGACGG TTCGGCGCCG CTGAGGTGTC 2761 AATCGTCGGA GCCGCTGAGC AATAACTAGC ATAACCCCTT GGGGCCTCTA AACGGGTCTT 2821 GAGGGGTTTT TTGCATGGTC ATAGCTGTTT CCTGAGAGCT TGGCAGGTGA TGACACACAT 2881 TAACAAATTT CGTGAGGAGT CTCCAGAAGA ATGCCATTAA TTTCCATAGG CTCCGCCCCC 2941 CTGACGAGCA TCACAAAAAT CGACGCTCAA GTCAGAGGTG GCGAAACCCG ACAGGACTAT 3001 AAAGATACCA GGCGTTTCCC CCTGGAAGCT CCCTCGTGCG CTCTCCTGTT CCGACCCTGC 3061 CGCTTACCGG ATACCTGTCC GCCTTTCTCC CTTCGGGAAG CGTGGCGCTT TCTCATAGCT 3121 CACGCTGTAG GTATCTCAGT TCGGTGTAGG TCGTTCGCTC CAAGCTGGGC TGTGTGCACG 3181 AACCCCCCGT TCAGCCCGAC CGCTGCGCCT TATCCGGTAA CTATCGTCTT GAGTCCAACC 3241 CGGTAAGACA CGACTTATCG CCACTGGCAG CAGCCACTGG TAACAGGATT AGCAGAGCGA 3301 GGTATGTAGG CGGTGCTACA GAGTTCTTGA AGTGGTGGCC TAACTACGGC TACACTAGAA 3361 GAACAGTATT TGGTATCTGC GCTCTGCTGA AGCCAGTTAC CTTCGGAAAA AGAGTTGGTA 3421 GCTCTTGATC CGGCAAACAA ACCACCGCTG GTAGCGGTGG TTTTTTTGTT TGCAAGCAGC 3481 AGATTACGCG CAGAAAAAAA GGATCTCAAG AAGGCCTACT ATTAGCAACA ACGATCCTTT 3541 GATCTTTTCT ACGGGGTCTG ACGCTCAGTG GAACGAAAAC TCACGTTAAG GGATTTTGGT 3601 CATGAGATTA TCAAAAAGGA TCTTCACCTA GATCCTTTTA AATTAAAAAT GAAGTTTTAA 3661 ATCAATCTAA AGTATATATG AGTAAACTTG GTCTGACAGT TACCAATGCT TAATCAGTGA 3721 GGCACCTATC TCAGCGATCT GTCTATTTCG TTCATCCATA GTTGCCTGAC TCCCCGTCGT 3781 GTAGATAACT ACGATACGGG AGGGCTTACC ATCTGGCCCC AGTGCTGCAA TGATACCGCG 3841 AGAACCACGC TCACCGGCTC CAGATTTATC AGCAATAAAC CAGCCAGCCG GAAGGGCCGA 3901 GCGCAGAAGT GGTCCTGCAA CTTTATCCGC CTCCATCCAG TCTATTAATT GTTGCCGGGA 3961 AGCTAGAGTA AGTAGTTCGC CAGTTAATAG TTTGCGCAAC GTTGTTGCCA TTGCTACAGG 4021 CATCGTGGTG TCACGCTCGT CGTTTGGTAT GGCTTCATTC AGCTCCGGTT CCCAACGATC 4081 AAGGCGAGTT ACATGATCCC CCATGTTGTG CAAAAAAGCG GTTAGCTCCT TCGGTCCTCC 4141 GATCGTTGTC AGAAGTAAGT TGGCCGCAGT GTTATCACTC ATGGTTATGG CAGCACTGCA 4201 TAATTCTCTT ACTGTCATGC CATCCGTAAG ATGCTTTTCT GTGACTGGTG AGTACTCAAC 4261 CAAGTCATTC TGAGAATAGT GTATGCGGCG ACCGAGTTGC TCTTGCCCGG CGTCAATACG 4321 GGATAATACC GCGCCACATA GCAGAACTTT AAAAGTGCTC ATCATTGGAA AACGTTCTTC 4381 GGGGCGAAAA CTCTCAAGGA TCTTACCGCT GTTGAGATCC AGTTCGATGT AACCCACTCG 4441 TGCACCCAAC TGATCTTCAG CATCTTTTAC TTTCACCAGC GTTTCTGGGT GAGCAAAAAC 4501 AGGAAGGCAA AATGCCGCAA AAAAGGGAAT AAGGGCGACA CGGAAATGTT GAATACTCAT 4561 ACTCTTCCTT TTTCAATATT ATTGAAGCAT TTATCAGGGT TATTGTCTCA TGAGCGGATA 4621 CATATTTGAA TGTATTTAGA AAAATAAACA AATAGGGGTT CCGCGCACCT GCACCAGTCA 4681 GTAAAACGAC GGCCAGTAGT CAAAAGCCTC CGACCGGAGG CTTTTGACTT GGTTCAGGTG 4741 GAGTGGGAGA ACGATGATGC TCACTCTCGG GTCTCA //